Erratum to Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia [Journal of Psychiatric Research (2009); 43(11): 978–86]

Laboratório de Neurociências, Instituto de Psiquiatria, Faculdade de Medicina da USP, Rua Dr. Ovídio Pires de Campos, SP, Brazil.
Journal of Psychiatric Research (Impact Factor: 3.96). 07/2009; 43(11):978-86. DOI: 10.1016/j.jpsychires.2008.11.006
Source: PubMed


Schizophrenia is likely to be a consequence of serial alterations in a number of genes that, together with environmental factors, will lead to the establishment of the illness. The dorsolateral prefrontal cortex (Brodmann's Area 46) is implicated in schizophrenia and executes high functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts, correct social behavior and personality expression. We performed a comparative proteome analysis using two-dimensional gel electrophoresis of pools from 9 schizophrenia and 7 healthy control patients' dorsolateral prefrontal cortex aiming to identify, by mass spectrometry, alterations in protein expression that could be related to the disease. In schizophrenia-derived samples, our analysis revealed 10 downregulated and 14 upregulated proteins. These included alterations previously implicated in schizophrenia, such as oligodendrocyte-related proteins (myelin basic protein and transferrin), as well as malate dehydrogenase, aconitase, ATP synthase subunits and cytoskeleton-related proteins. Also, six new putative disease markers were identified, including energy metabolism, cytoskeleton and cell signaling proteins. Our data not only reinforces the involvement of proteins previously implicated in schizophrenia, but also suggests new markers, providing further information to foster the comprehension of this important disease.

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    • "DPYSL2, which acts in the regulation of axon guidance, vesicle trafficking and synaptic function, has been shown to bind and be modulated by antidepressants and neuroactive molecules [19]. INA and DPYSL2 have been found differentially expressed in schizophrenia brains [34, 35, 41]. While their phosphorylation status has not been mentioned on schizophrenia, differences in expression of these proteins have not been related to MDD so far. "
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    ABSTRACT: There is still a lack in the molecular comprehension of major depressive disorder (MDD) although this condition affects approximately 10% of the world population. Protein phosphorylation is a posttranslational modification that regulates approximately one-third of the human proteins involved in a range of cellular and biological processes such as cellular signaling. Whereas phosphoproteome studies have been carried out extensively in cancer research, few such investigations have been carried out in studies of psychiatric disorders. Here, we present a comparative phosphoproteome analysis of postmortem dorsolateral prefrontal cortex tissues from 24 MDD patients and 12 control donors. Tissue extracts were analyzed using liquid chromatography mass spectrometry in a data-independent manner (LC-MSE). Our analyses resulted in the identification of 5,195 phosphopeptides, corresponding to 802 non-redundant proteins. Ninety of these proteins showed differential levels of phosphorylation in tissues from MDD subjects compared to controls, being 20 differentially phosphorylated in at least 2 peptides. The majority of these phosphorylated proteins were associated with synaptic transmission and cellular architecture not only pointing out potential biomarker candidates but mainly shedding light to the comprehension of MDD pathobiology. Electronic supplementary material The online version of this article (doi:10.1007/s00406-012-0301-3) contains supplementary material, which is available to authorized users.
    European Archives of Psychiatry and Clinical Neuroscience 02/2012; 262(8). DOI:10.1007/s00406-012-0301-3 · 3.53 Impact Factor
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    • "MRS studies of patients with chronic schizophrenia as well as at first episode prior to treatment showed alterations in neuronal membrane biochemistry, including the prefrontal cortex [63]. The results of both metabolomic and proteomic studies pointed to energy metabolism and lipid biosynthesis [64], alterations of free fatty acids, phosphatidylcholines, and ceramides [65], cytoskeleton, oligodendrocyte, energy metabolism and cell-signalling proteins being impaired in schizophrenia [66]. Tkachev et al. [67] provide evidence for altered myelin biosynthesis and glutamatergic dysfunction in the prefrontal cortex in schizophrenia. "
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    ABSTRACT: Schizophrenia is believed to result from altered neuronal connectivity and impaired myelination. However, there are few direct evidence for myelin abnormalities in schizophrenia. We performed electron microscopic study of myelinated fibers and oligodendrocytes and morphometric study of myelinated fibers in the prefrontal cortex in gray and white matters in schizophrenia and normal controls. Six types of abnormal fibers and ultrastructural alterations of oligodendrocytes were found in schizophrenia. No significant group differences in area density of myelinated fibers were found. Frequency of pathological fibers was increased significantly in gray matter in young and elderly schizophrenia patients and in patients with predominantly positive symptoms. In contrast, in white matter, frequency of altered fibers was increased significantly in elderly patients, in patients with predominantly negative symptoms, and correlated with illness duration. Progressive alterations of myelinated fibers in white matter might be followed by alterations of myelinated fibers in gray matter in schizophrenia.
    06/2011; 2011(2):325789. DOI:10.1155/2011/325789
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    • "2DE has been extensively and successfully used in studies of brain tissue or body fluids of psychiatric disorders such as SCZ [46, 49, 60] and BPD [2, 17] as well as in studies of neurodegenerative disorders such as Alzheimer’s disease (AD) [25, 72, 74]. These studies have identified a number of proteins that could be involved in disease pathogenesis, increasing the understanding of such disorders. "
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