Phytochemical Screening, Flavonoid Content and Antioxidant Activity of Tiliacora Triandra Leaf Extracts


Tiliacora triandra (Menispermaceae) is used in traditional medicine as antipyretic and anti-cancer agents. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the leaf extracts. Antioxidant were determined by the DPPH scavenging and the ferric reducing antioxidant power (FRAP) methods. Phytochemical screening was performed using standard procedures. Flavonoid content was measured by the aluminum chloride colorimetric assay. The results showed that the methanol extract had the highest antioxidant activity in both methods when compared to the other extracts. Phytochemical screening of the methanol extract showed the presence of alkaloid, flavonoid, tannin and saponins. Flavonoid content was found to be 18.67+0.28 mg quercetin equivalent/ g of extract. In conclusion, the apparent antioxidant activitiy suggest its potential usefulness in the prevention of some diseases. Introduction Several research provide strong evidence that plant is a source of inspiration for novel plant compounds, as plant derived compound have made large contributions to human health as well being. Antioxidants derived from fruits, vegetables, herbs, and beverages has been increasing interest (Pourmorad, 2006) for prevented of some disease such as coronary heart disease (Hertog, 1997 and Giugliano, 2000), stoke (Keli,1996), and cancer (Hollman, 1999). In Thailand, the people consume edible plants as part of their diet. Tiliacora triandra Diels,known in Thai as Ya-nang, belongs to Menispermaceae family. In traditional Thai medicine, its root has been used as antipyretic agent for all kind of fever. The leaf juice has been used traditionally for anticancer and immunomodilator 8 . Previous phytochemical studies in its root revealed some bisbenzylisoquinoline alkaloids including tiliacorine and tiliacorinine (Chalerm, 2003 and Katchrinnee, 2006) but there has been minimal investigation of its chemical constituents in its leaves. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the Tiliacora triandra's leaf extracts. Methods and Materials Plant material and extraction The plant sample (leaves) of T. triandra was collected from the Mahasarakham province of Thailand and identified by the authors. The sample was extracted with petroleum ether, dichloromethane, ethyl acetate, methanol in a soxhlet apparatus, respectively. Another sample was macerated with distilled water. The organic solvents were evaporated by a rotary evaporator while the water extract was lyophilized.

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    • "According to the traditional medicine of many countries in Southeast Asia, it has been used as anti-pyretic, detoxication agent, anti-inflammation, anticancer, antibacterial, and immune modulator. In addition, it also possesses antioxidant activity [13]. Recent toxicity study has revealed that water extract of T. triandra leaves shows no toxicity up to 5000 mg⋅kg −1 in single administration. "
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    ABSTRACT: Oxidative stress plays an important role in brain dysfunctions induced by alcohol. Since less therapeutic agent against cognitive deficit and brain damage induced by chronic alcohol consumption is less available, we aimed to assess the effect of Tiliacora triandra extract, a plant possessing antioxidant activity, on memory impairment, neuron density, cholinergic function, and oxidative stress in hippocampus of alcoholic rats. Male Wistar rats were induced ethanol dependence condition by semivoluntary intake of alcohol for 15 weeks. Alcoholic rats were orally given T. triandra at doses of 100, 200, and 400 mg·kg−1BW for 14 days. Memory assessment was performed every 7 days while neuron density, activities of AChE, SOD, CAT, and GSH-Px and, MDA level in hippocampus were assessed at the end of study. Interestingly, the extract mitigated the increased escape latency, AChE and MDA level. The extract also mitigated the decreased retention time, SOD, CAT, and GSH-Px activities, and neurons density in hippocampus induced by alcohol. These data suggested that the extract improved memory deficit in alcoholic rats partly via the decreased oxidative stress and the suppression of AChE. Therefore, T. triandra is the potential reagent for treating brain dysfunction induced by alcohol. However, further researches are necessary to understand the detail mechanism and possible active ingredient.
    Oxidative medicine and cellular longevity 07/2015; 2015:1-9. DOI:10.1155/2015/918426 · 3.36 Impact Factor