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Phytochemical Screening, Flavonoid Content and Antioxidant Activity of Tiliacora Triandra Leaf Extracts

ABSTRACT Tiliacora triandra (Menispermaceae) is used in traditional medicine as antipyretic and anti-cancer agents. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the leaf extracts. Antioxidant were determined by the DPPH scavenging and the ferric reducing antioxidant power (FRAP) methods. Phytochemical screening was performed using standard procedures. Flavonoid content was measured by the aluminum chloride colorimetric assay. The results showed that the methanol extract had the highest antioxidant activity in both methods when compared to the other extracts. Phytochemical screening of the methanol extract showed the presence of alkaloid, flavonoid, tannin and saponins. Flavonoid content was found to be 18.67+0.28 mg quercetin equivalent/ g of extract. In conclusion, the apparent antioxidant activitiy suggest its potential usefulness in the prevention of some diseases. Introduction Several research provide strong evidence that plant is a source of inspiration for novel plant compounds, as plant derived compound have made large contributions to human health as well being. Antioxidants derived from fruits, vegetables, herbs, and beverages has been increasing interest (Pourmorad, 2006) for prevented of some disease such as coronary heart disease (Hertog, 1997 and Giugliano, 2000), stoke (Keli,1996), and cancer (Hollman, 1999). In Thailand, the people consume edible plants as part of their diet. Tiliacora triandra Diels,known in Thai as Ya-nang, belongs to Menispermaceae family. In traditional Thai medicine, its root has been used as antipyretic agent for all kind of fever. The leaf juice has been used traditionally for anticancer and immunomodilator 8 . Previous phytochemical studies in its root revealed some bisbenzylisoquinoline alkaloids including tiliacorine and tiliacorinine (Chalerm, 2003 and Katchrinnee, 2006) but there has been minimal investigation of its chemical constituents in its leaves. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the Tiliacora triandra's leaf extracts. Methods and Materials Plant material and extraction The plant sample (leaves) of T. triandra was collected from the Mahasarakham province of Thailand and identified by the authors. The sample was extracted with petroleum ether, dichloromethane, ethyl acetate, methanol in a soxhlet apparatus, respectively. Another sample was macerated with distilled water. The organic solvents were evaporated by a rotary evaporator while the water extract was lyophilized.

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Jun 2, 2014