Phytochemical Screening, Flavonoid Content and Antioxidant Activity of Tiliacora Triandra Leaf Extracts

ABSTRACT Tiliacora triandra (Menispermaceae) is used in traditional medicine as antipyretic and anti-cancer agents. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the leaf extracts. Antioxidant were determined by the DPPH scavenging and the ferric reducing antioxidant power (FRAP) methods. Phytochemical screening was performed using standard procedures. Flavonoid content was measured by the aluminum chloride colorimetric assay. The results showed that the methanol extract had the highest antioxidant activity in both methods when compared to the other extracts. Phytochemical screening of the methanol extract showed the presence of alkaloid, flavonoid, tannin and saponins. Flavonoid content was found to be 18.67+0.28 mg quercetin equivalent/ g of extract. In conclusion, the apparent antioxidant activitiy suggest its potential usefulness in the prevention of some diseases. Introduction Several research provide strong evidence that plant is a source of inspiration for novel plant compounds, as plant derived compound have made large contributions to human health as well being. Antioxidants derived from fruits, vegetables, herbs, and beverages has been increasing interest (Pourmorad, 2006) for prevented of some disease such as coronary heart disease (Hertog, 1997 and Giugliano, 2000), stoke (Keli,1996), and cancer (Hollman, 1999). In Thailand, the people consume edible plants as part of their diet. Tiliacora triandra Diels,known in Thai as Ya-nang, belongs to Menispermaceae family. In traditional Thai medicine, its root has been used as antipyretic agent for all kind of fever. The leaf juice has been used traditionally for anticancer and immunomodilator 8 . Previous phytochemical studies in its root revealed some bisbenzylisoquinoline alkaloids including tiliacorine and tiliacorinine (Chalerm, 2003 and Katchrinnee, 2006) but there has been minimal investigation of its chemical constituents in its leaves. The current study sought to evaluate the antioxidant activity, to screen their chemical constituents and to investigate the flavonoid content of the Tiliacora triandra's leaf extracts. Methods and Materials Plant material and extraction The plant sample (leaves) of T. triandra was collected from the Mahasarakham province of Thailand and identified by the authors. The sample was extracted with petroleum ether, dichloromethane, ethyl acetate, methanol in a soxhlet apparatus, respectively. Another sample was macerated with distilled water. The organic solvents were evaporated by a rotary evaporator while the water extract was lyophilized.

Download full-text


Available from: Surapong Rattana, Sep 28, 2015
1,465 Reads
  • Journal of Rural Medicine 01/2005; 1(1):4-14. DOI:10.2185/jrm.1.4
  • [Show abstract] [Hide abstract]
    ABSTRACT: Leaves, fruits, flowers and stem bark extracts from the Siamese neem tree (Azadirachta indica A. Juss var. siamensis Valeton, Meliaceae) were assessed for antioxidant activity in vitro using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay, total antioxidant activity and inhibition of lipid peroxidation in Chago K1 cancer cell culture by the thiobarbituric acid reactive substances (TBARS) method. The results showed that leaf aqueous extract, flower and stem bark ethanol extracts exhibited higher free radical scavenging effect on the DPPH assay with 50% scavenging activity at 26.5, 27.9 and 30.6 microg/ml, respectively. The total antioxidant activity of these extracts was found to be 0.959, 0.988 and 1.064 mM of standard trolox, respectively. At 100 microg/ml, the flower ethanol and leaf aqueous extracts significantly decreased malondialdehyde (MDA) levels (46.0 and 50.6%, respectively) by the TBARS method. The results suggest that extracts from leaf, flower and stem bark of the Siamese neem tree have strong antioxidant potential. This report supports the ethnomedical use of young leaves and flowers of this plant as a vegetable bitter tonic to promote good health.
    Journal of Ethnopharmacology 06/2005; 99(1):109-12. DOI:10.1016/j.jep.2005.02.008 · 3.00 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The research objectives were to quantify the amount of proanthocyanidins and determine the antioxidant property of grape seeds, cinsaut variety, cultivated in Nakhon Ratchasima province. The defatted grape seeds powder was extracted by 2 methods. The first method was soxhlet extraction with ethanol, and the product hereby was a precipitate of crude proanthocyanidins 0.223%. The second method was maceration with ethylacetate and water (90:10). The extract was concentrated under vacuum and then redissolved in ethylacetate. The crude proanthocyanidins were precipitated out 0.216%, when petroleum ether was added into this crude extract solution. The antioxidant activities of these two crude proanthocyanidins were compared with those of L-ascorbic acid and of trolox by spectrophotometry using Ferric Reducing/Antioxidant Power assay. The antioxidant activities per milligram of L-ascorbic acid, trolox and crude proanthocyanidins by soxhlet and maceration were equal to 14.01, 10.50, 2.98 and 3.22 mmole of Fe 2+ , respectively. The total amounts of flavonols in crude proanthocyanidins from soxhlet extraction and maceration were 48.47% and 46.28%, respectively, calculated in catechin equivalent by vanillin-HCl assay.