Anatomia de raiz, caule e folha e identificação de estruturas secretoras de Achillea millefolium L. (Asteraceae) - DOI: 10.4025/actascibiolsci.163

Acta Scientiarum Biological Sciences 01/2006; 28(4):327-334. DOI: 10.4025/actascibiolsci.v28i4.163

ABSTRACT An anatomic study of the vegetative organs: root, stem and leaf was made. The essential oils were extracted from the plant identifying the possible secretory structures. Fresh material was used in order to perform histochemical tests and to prepare semi-permanent slides, which were colored with Safrabau. For the preparation of the semi-permanent slides, material fixed in FAA 50% was used including historesina. Tector hairs can be observed in the scape and flower. Secretory channels of schizogeneous origin follow the vascular bundles in the rhizome, scape, leaf and bract of the flower. In the root, these channels are absent. In the leaf and in the unopened inflorescence there is a greater quantity of the essential oils. The leaf is amphistomatic and the stomata of anomocytic type are present in the scape as well. The general structure of the Achillea millefolium is similar to the structures of the other species of Asteraceae family.

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    ABSTRACT: The ultrastructure of the glandular cells of the floret secretory trichomes from Achillea millefolium L. ssp. millefolium (yarrow) was examined before and after anthesis and compared with the ultrastructure of the cells from the cell suspension cultures obtained from the same plant. The profuse tubular structures observed in the plastids of the glandular cells of the trichomes during the pre-secretory stage were much reduced in the secretory stage and showed an osmiophilic content. Some endoplasmic reticulum profiles could be seen adjacent to the plastids. Later in the secretory stage, the secretion appeared in the periplasmic space between the cells of the upper tiers and in the sub-cuticular space. Finally the secretion was released by rupture of the cuticle. At the lag phase, the cells from the cell suspension cultures of yarrow were characterized by the presence or plastids with tubular structures, similar to those observed in the plastids of the trichomes in the pre-secretory stage. By the end of the lag phase accumulations of starch were observed inside the plastids. At the beginning of the exponential phase, the tubular structures of the plastids started to show an osmiophilic content and the accumulations of starch were still present. At the end of this phase starch disappeared from the plastids and only osmiophilic tubular structures were observed. Rough endoplasmic reticulum as well as smooth endoplasmic reticulum profiles were frequently in close association with plastids and mitochondria. At the stationary phase a very large vacuole filled the cells, and in the remaining cytoplasm some endoplasmic reticulum profiles and osmiophilic droplets were observed. Copyright 1994, 1999 Academic Press
    Annals of Botany 08/1994; 74(2). DOI:10.1006/anbo.1994.1107 · 3.30 Impact Factor
  • Lloydia 01/1975; 37(4):598-602.
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    ABSTRACT: The composition of the essential oils from leaves and from flowers of Achillea millefolium L. ssp. millefolium was analysed by GC and GC-MS. During the flowering period of the plant, both oils consisted mainly of monoterpenes (about 80%). 1,8-Cineole was the dominant component in the oils from the flowers (29%) and from the leaves (25%), while sabinene was the second most important component in the oil from the flowers (15%) and trans-sabinene hydrate in that from the leaves (10%). The sesquiterpene fraction was in both cases dominated by germacrene-D (0.7% in the oil of the flowers and 7% in the leaf oil).In the essential oil isolated from leaves collected during the vegetative phase, the monoterpene fraction was small (<3%), whereas sesquiterpenes amounted to 92%, germacrene-D being the major component (65%) of the oil.
    Flavour and Fragrance Journal 08/1992; 7(4):219 - 222. DOI:10.1002/ffj.2730070409 · 1.76 Impact Factor


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