Revista Brasileira de Ciência do Solo (Impact Factor: 0.76). 01/2001; 25(4):873-883. DOI: 10.1590/S0100-06832001000400010


Soil acidity is one of the greatest problems faced in the management of agricultural lands around the world, mainly in the tropics, where phosphorus and nitrogen are also limiting factors. In the case of nitrogen, biological nitrogen fixation is the most economical and ecological alternative to decrease the use and impact caused by nitrogen fertilizers. Two "in vitro" experiments and one under greenhouse conditions using four Bradyrhizobium strains (BR 4406, BR 29, SEMIA 587 and INPA 03-11B) were carried out at the Soil Science Department, at Federal University of Lavras from July 1998 to July 1999, aiming to verify the effect of three pH values (5.0; 6.0 and 6.9) on growth of Bradyrhizobium strains in YM medium, soybean symbiosis and peat survival. In the first experiment, Bradyrhizobium strains had diffentiated behaviour at the three pHs of YM medium, and had the highest colony forming unities and exopolysaccharide production at pH 6.0. In the second experiment, nodule number (NN), nitrogenase (Nase) activity, dry matter of nodules (NDM), roots (RDM) and shoots (SDM) of soybean were not affected by the different pHs of the culture medium used to produce peat inoculants. INPA 03-11B had similar efficiency to BR 29 and SEMIA 587, recommended as inoculant strains for soybean, on the production of NN, Nase activity, NDM, RDM and SDM. In the third experiment, all strains had the same cell survival at pH 6 and 6.9, except BR 29, which had the highest survival at pH 6.0. The best behaviour of Bradyrhizobium strains at pH 6.0 in culture medium and in peat survival indicated the possibility to use this pH in the peat inoculant production as a means of acid adaptation to this soil stress condition in the tropics.

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Available from: Fatima Maria de Souza Moreira, Jan 04, 2014
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    01/2014; 18(6):567-573. DOI:10.1590/S1415-43662014000600001
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    ABSTRACT: The common bean (Phaseolus vulgaris) has the capacity to benefit from biological nitrogen fixation (FBN). However the success of this process is influenced by several factors. The aim of this study was to scale the magnitude of the contribution of molybdenum (sources and doses) and rhizobia (native and introduced) for nodulation, nitrogen accumulation and growth of IPR 139 bean. The experiment was conducted in 2011 in a greenhouse in a completely randomized factorial 2 x 5 x 2, corresponding respectively to the application in seeds from two sources (sodium molybdate and ammonium molybdate) and five doses of Mo (0, 1, 2, 3 and 4 g kg(-1) seed) in the presence and absence of rhizobia inoculation (Rhizobium tropici SEMIA 4088). The variables analysed were: dry mass of shoot, root and total (shoot + root), dry nodules and average unit nodule, the total number of nodules and total nitrogen in aerial parts. The results indicate ammonium molybdate as the best source for raising the mass formed by indigenous rhizobia nodules, at doses between 2 and 3 g kg(-1) seed. Inoculation of R. tropici SEMIA 4088 was not efficient to increase plant growth, nor nodulation and nitrogen fixation.
    06/2014; 18(6):567-573.