Bone morphogenetic protein 7 (BMP-7) increases the expression of follicle-stimulating hormone (FSH) receptor in human granulosa cells

Department of Obstetrics and Gynecology, University of Tokyo, Tokyo 113-8655, Japan.
Fertility and sterility (Impact Factor: 4.59). 01/2009; 93(4):1273-9. DOI: 10.1016/j.fertnstert.2008.11.014
Source: PubMed


To examine the effect of bone morphogenetic protein 7 (BMP-7) on FSH receptor (FSHR) expression in human granulosa cells.
Laboratory study using human samples.
University hospital.
Human granulosa cells were obtained from 60 women undergoing oocyte retrieval for IVF.
Human granulosa cells (GCs) were cultured with recombinant BMP-7, followed by RNA extraction.
mRNA levels of GCs were measured by real-time reverse-transcription polymerase chain reaction.
Bone morphogenetic protein 7 increased FSHR gene expression in human luteinized granulosa cells, whereas it decreased LH receptor gene expression. Bone morphogenetic protein 7 also increased FSH-induced cyclic adenosine monophosphate production in GCs, indicating up-regulation of the cellular response to FSH. Although BMP-7 increased gene expression of activin-betaA and -betaB in GCs, inhibition of activin function did not affect the BMP-7-induced FSHR gene expression.
These findings provide new insight into the biologic function of BMP-7 in the human ovary and demonstrate its unique mechanism of regulating FSHR action.

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    • "The authors furthermore found that growth of cultured human granulosa cells was linked to the presence of GDF-3, BMP-6 or BMP-7. In a previous study with in vitro human granulosa cells, Shi et al. (2010) reported a BMP-7 induced reduction in LH receptor gene expression. In addition, cultured human granulosa cells show a reduction in the order of 50–60% in the expression of PCSK6, when cultured in the presence of BMP-2, -6, -7 or -15 (Akiyama et al., 2012). "
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    • "Primary human GCs were obtained from patients undergoing transvaginal oocyte retrieval for in vitro fertilization at the University of Tokyo Hospital. The method to purify human GCs was described previously [15]. The study was approved by the Institutional Review Board of the University of Tokyo, and written informed consent for the research use of human GCs was obtained from each patient. "
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