Development of quantitative real-time PCR primers for detecting 42 oral bacterial species.

Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seo-suk Dong, Dong-Gu, Gwangju, 501-759, Republic of Korea.
Archives of Microbiology (Impact Factor: 1.91). 05/2013; DOI: 10.1007/s00203-013-0896-4
Source: PubMed

ABSTRACT In this study, we introduced species-specific quantitative real-time PCR (qPCR) primers designed based on a DNA-dependent RNA polymerase beta-subunit gene (rpoB) for detecting 42 oral bacterial species. The specificity of the qPCR primers was confirmed by conventional PCR with the genomic DNAs of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. The standard curves revealed the lower detection limits of 42 bacterial species-specific qPCR primers ranged from 4 to 40 fg below a cycle threshold (C T) value of 35, except Atopobium rimae, Fusobacterium nucleatum, Neisseria meningitidis, and Porphyromonas asaccharolytica which were 400 fg. These results suggest that 42 bacterial species-specific qPCR primers are suitable for applications in epidemiological studies related to oral infectious diseases such as periodontal diseases, endodontic infection, and dental caries.

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May 20, 2014