Article

In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus.

Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Journal of Oral Science 12/2008; 50(4):397-402. DOI: 10.2334/josnusd.50.397
Source: PubMed

ABSTRACT The purpose of the present in vitro study was to compare the cytotoxic effect of two commercially available brands of mineral trioxide cement (ProRoot MTA and MTA Angelus), modified zinc oxide-eugenol cement (SuperEBA) and resin-modified glass ionomer cement (Vitrebond) using rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC-5). The cells were cultured in typical culture conditions and exposed to the tested materials by adaptation of insert wells. The cytotoxic effect was recorded at two observation periods (24 and 72 h) by using a colorimetric assay of tetrazolium reduction (XTT method) in reference to controls. Overall, the degree of cytotoxic effect in ascending order was ProRoot MTA - MTA Angelus < SuperEBA < Vitrebond. Both MTA materials tested exerted mild suppression of cellular mitochondrial activity and may be characterized as biologically inert materials.

0 Bookmarks
 · 
228 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Root canal perforation and root resorption are challenging clinical conditions to correctly diagnose and treat, especially when they occur in anterior teeth. This clinical report describes the computed tomography findings, endodontic treatment, prosthetic rehabilitation, and clinical outcome of an iatrogenic root perforation and internal resorption in a maxillary central incisor. The case management consisted of endodontic retreatment, periodontal surgery, and prosthetic rehabilitation. Gray mineral trioxide aggregate (MTA) was used to fill the resorption space and seal the perforation. The prosthetic treatment was performed with glass fiber-reinforced dowels and all-ceramic crowns. No signs or symptoms, including discomfort, pain, or esthetic defects were observed in 30 months of follow-up.
    Journal of Prosthodontics 02/2013; · 0.68 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this bench top evidence level 5 in vitro study was to compare the cytotoxic effect of 2 brands of white mineral trioxide aggregate cement (ProRoot MTA and MTA-Angelus), Brasseler EndoSequence Root Repair Material, and Brasseler EndoSequence Root Repair Putty by using human dermal fibroblasts. The cells were cultured in recommended culture conditions and exposed to the tested materials. The cytotoxic effects were recorded at an observation period of 24 hours by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based colorimetric assay. Results were analyzed by using one-way analysis of variance with significance of p < .05. All materials tested demonstrated cell viability ≥ 91.8%. Overall, there was no statistically significant difference in cell viability of ProRoot MTA, MTA-Angelus, and Brasseler EndoSequence Root Repair Material. However, there was a statistically significant difference negatively associated with the cell viability of human dermal fibroblasts in association with the Brasseler EndoSequence Root Repair Putty. The Brasseler EndoSequence Root Repair Materials were shown to have similar cytotoxicity levels to those of ProRoot MTA and MTA-Angelus.
    Journal of endodontics 03/2011; 37(3):372-5. · 2.95 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The mechanisms of free fatty acid (FFA)-induced peripheral insulin resistance remain elusive. This study aimed to investigate the effect of palmitate, a saturated fatty acid, on glucose metabolism in C2C12 myotubes, and to explore the underlying mechanisms. In it, palmitate decreased insulin-stimulated glucose uptake and consumption in a dose-dependent manner, and it reduced the insulin-stimulated phosphorylation of Akt at Thr308 and Ser473, but had no effect on the protein expression of PI3K-p85 or the activity of PI3K. Additionally, it inhibited the insulin-stimulated phosphorylation of Src at Tyr416, causing a reduction in the Src-mediated phosphorylation of Akt. Inhibition of Src by PP2 resulted in decreases in insulin-stimulated glucose uptake and phosphorylation of Src at Tyr416 and Akt at Thr308 and Ser473. The findings indicate that palmitate contributes to insulin resistance by inhibiting the Src-mediated phosphorylation of Akt in C2C12 myotubes, and this provides insight into the molecular mechanisms of FFA-induced insulin resistance.
    Bioscience Biotechnology and Biochemistry 07/2012; 76(7):1356-61. · 1.27 Impact Factor

Full-text

View
1 Download
Available from