Article

In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA

Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Journal of Oral Science (Impact Factor: 0.92). 12/2008; 50(4):397-402. DOI: 10.2334/josnusd.50.397
Source: PubMed

ABSTRACT The purpose of the present in vitro study was to compare the cytotoxic effect of two commercially available brands of mineral trioxide cement (ProRoot MTA and MTA Angelus), modified zinc oxide-eugenol cement (SuperEBA) and resin-modified glass ionomer cement (Vitrebond) using rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC-5). The cells were cultured in typical culture conditions and exposed to the tested materials by adaptation of insert wells. The cytotoxic effect was recorded at two observation periods (24 and 72 h) by using a colorimetric assay of tetrazolium reduction (XTT method) in reference to controls. Overall, the degree of cytotoxic effect in ascending order was ProRoot MTA - MTA Angelus < SuperEBA < Vitrebond. Both MTA materials tested exerted mild suppression of cellular mitochondrial activity and may be characterized as biologically inert materials.

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    • "These were added to the culture wells using sterile forceps. All the samples were subsequently incubated at 37°C with 5% CO 2 in DMEM following a 1 h ultraviolet exposure [8] [9] [10]. The elutes of materials were prepared separately and were incubated in DMEM-F12 at 37°C in a 5% CO 2 for 1, 2, 3 and 7 days [11]. "
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    • "While both the white and gray MTA-Angelus have arsenic in their structures, white MTA-Angelus has the benefit of containing arsenic levels below the limit set by the ISO 9917-1 standard.[20] Like ProRoot MTA, MTA-Angelus has low cytotoxicity.[21] "
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