Article

Relationship between first polar body morphology before intracytoplasmic sperm injection and fertilization rate, cleavage rate, and embryo quality.

Department of Obstetrics and Gynecology, Ribeirão Preto Medical School, São Paulo University, Ribeirão Preto, São Paulo, Brazil.
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics (impact factor: 1.41). 12/2008; 104(3):226-9. DOI:10.1016/j.ijgo.2008.11.008 pp.226-9
Source: PubMed

ABSTRACT To evaluate the influence of the morphology of the first polar body (PB) on intracytoplasmic sperm injection (ICSI) outcomes.
The morphology of the first PB was assessed in 3177 metaphase II oocytes and classified as: intact and normal size, fragmented, or enlarged size. The rates of fertilization, cleavage, and embryo quality were evaluated on day 2.
The rates of fertilization, cleavage, and formation of good quality embryos resulting from the insemination of oocytes with an enlarged first PB (20.7%, 18.7%, and 5.0%, respectively) were significantly lower than those for oocytes with an intact first PB of normal size (70.8%, 62.5%, and 19%, respectively) or a fragmented first PB (69.7%, 60.5%, and 17.1%, respectively). Rates did not differ significantly between oocytes with an intact first PB of normal size and oocytes with a fragmented first PB (P>0.05).
The presence of an enlarged PB is related to poorer rates of fertilization, cleavage, and top quality embryos. However, identification of first PB fragmentation does not seem to interfere with ICSI outcomes.

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Keywords

3177 metaphase II oocytes
 
day 2
 
embryo quality
 
enlarged first PB
 
enlarged PB
 
enlarged size
 
first PB
 
first PB fragmentation
 
first polar body
 
fragmented first PB
 
good quality embryos
 
ICSI
 
ICSI outcomes
 
insemination
 
intact first PB
 
intracytoplasmic sperm injection
 
normal size
 
poorer rates
 
Rates
 
top quality embryos