Article

A scFv antibody-based immunoaffinity chromatography column for clean-up of bisphenol A-contaminated water samples.

Research Center for Environmental Genomics and Graduate School of Science and Technology, Kobe University, Rokkodaicho, Nada-ku, Kobe, Hyogo, Japan.
Journal of Agricultural and Food Chemistry (impact factor: 2.82). 01/2009; 57(2):353-8. DOI:10.1021/jf802781t
Source: PubMed

ABSTRACT This is a report on the development of immunoaffinity chromatography using a column of silica gel with an immobilized single-chain variable fragment (scFv) antibody specific to bisphenol A (BPA) for cleanup of BPA-contaminated water samples. The BBA-2187 scFv antibody specific to BPA was purified from the periplasmic fractions of the recombinant Escherichia coli. After a sample of BPA-contaminated river water was applied to the immunoaffinity column, the background signal intensity observed in high-performance liquid chromatography (HPLC) analysis of the eluates was markedly lower than that observed in HPLC analysis of the eluates from an Oasis HLB cartridge treated with the same sample. The immunoaffinity column efficiently concentrated BPA from actual river water samples with different matrices. Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources.

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Keywords

actual river water samples
 
background signal intensity
 
BBA-2187 scFv antibody specific
 
BPA
 
BPA-contaminated river water
 
BPA-contaminated water samples
 
different matrices
 
different sources
 
HPLC analysis
 
immobilized BBA-2187 scFv antibody
 
immobilized single-chain variable fragment
 
immunoaffinity column
 
periplasmic fractions
 
recombinant Escherichia coli
 
silica gel