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Generation of Rat and Human Induced Pluripotent Stem Cells by Combining Genetic Reprogramming and Chemical Inhibitors

Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cell stem cell (Impact Factor: 22.15). 04/2009; 4(1):16-9. DOI: 10.1016/j.stem.2008.11.014
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Available from: Saiyong Zhu, Jun 13, 2014
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    • "Engineered endonucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, are invaluable tools for the rapid generation of GM animals including rats5678. Importantly, these new technologies provide genome-editing approaches for a wide variety of organisms that were previously inaccessible without embryonic stem (ES) cells91011 and induced pluripotent stem (iPS) cells1213. GM animals are usually produced by microinjecting engineered endonucleases into pronuclear-stage embryos56. Although this method is now the gold standard, it requires sophisticated manual skills to prevent cell damage. "
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