Article

Automated trapping, assembly, and sorting with holographic optical tweezers

Department of Chemical Engineering, Yale University New Haven, CT 06511, USA.
Optics Express (Impact Factor: 3.49). 01/2007; 14(26):13095-100. DOI: 10.1364/OE.14.013095
Source: PubMed

ABSTRACT We combine real-time feature recognition with holographic optical tweezers to automatically trap, assemble, and sort micron-sized colloidal particles. Closed loop control will enable new applications of optical micromanipulation in biology, medicine, materials science, and possibly quantum computation.

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Available from: Eric Dufresne, Mar 20, 2014
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    • "Optical trap arrays can be generated by several approaches, such as multi-beam interference [5] [14], phase contrast [15] [16] [17] [18], or Talbot self-imaging [19]. Most of the optical trap arrays for optical sorting are generated by holographic methods [20] [21] [22] [23] that use a diffractive optical element in the optical path to transform a single spot trap to the desired trap arrays, namely the holographic optical tweezers (HOT). Obviously, the generation of optical trap arrays is more complicated compared with the traditional single optical tweezers. "
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    • "Cells or other living systems need to be precisely micromanipulated in various applications such as, cell transport [1] [2] [3], sorting or separation [4] [5] [6] [7], estimation of mechanical properties [8], cell-cell interaction [9, 10], etc. Often, gradient centrifugation [11, 12], magnetic activated cell sorting [13], micro-fluidic techniques [14], etc. are utilized in many applications . However, there are two main limitations of these techniques , namely, (1) the need for a large sample size (except micro-fluidic techniques), and (2) they cannot be used for precise manipulation of a given target cell. "
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    • "Currently most of the optical tweezer systems use a mouse or a stand joystick to control the optical trap. While manual manipulation is robust, it cannot be efficiently used for applications that require precise manipulations of large numbers of cells repetitively or coordinated manipulations of multiple optical traps (Chapin et al., 2006). A few works have been done towards the automatic manipulation of optical trapping (Grover et al., 2001; Tanaka et al., 2008; Arai et al., 2009; Banerjee et al., 2010), in which, however , an open-loop control strategy was adopted to move cells along with pre-designed collision-free paths. "
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