Article

[Intervention of Poloxamer 188 to chondrocyte damaged by blunt mechanical trauma in vitro].

Department of Orthopaedics, China-Japan Friendship Hospital, Beijing 100029, China.
Zhonghua wai ke za zhi [Chinese journal of surgery] 09/2008; 46(16):1253-5. pp.1253-5
Source: PubMed

ABSTRACT To explore Poloxamer 188, a non-ionic surfactant as a potential tool for early intervention into the chondrocyte damaged by blunt mechanical trauma in vitro.
Three groups were control group (n = 6), no treatment group (n = 12) and Poloxamer 188 treatment group (n = 12). Two groups are then loaded to 20 MPa in unconfined compression. At 1 and 24 h the percentages of live and dead cells of superficial zone in compressed and control groups were determined with a cell viability stain.
At 1 h post-trauma, specimens of Poloxamer 188 treatment group (76%) had a significantly increased percentage of live cells in the superficial zone versus the no treatment group (55%). In 24 h the percentages of live cells in the superficial zone of the Poloxamer 188 treatment group (57%) were significantly greater than in the no treatment group (38%).
Poloxamer 188 surfactant could help restore the integrity of cell membranes in cartilage damaged by blunt mechanical trauma. Models of mechanical cartilage injury in vitro may explain aspect of the interactions between mechanical forces and degradative pathways which lead to osteoarthritis progression.

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Keywords

1 h post-trauma
 
cell viability stain
 
chondrocyte
 
compressed
 
control groups
 
degradative pathways
 
increased percentage
 
mechanical cartilage injury
 
mechanical forces
 
mechanical trauma
 
non-ionic surfactant
 
osteoarthritis progression
 
percentages
 
Poloxamer 188
 
Poloxamer 188 surfactant
 
Poloxamer 188 treatment group
 
superficial zone
 
treatment group
 
unconfined compression
 
vitro