GoldenBraid2.0: A comprehensive DNA assembly framework for Plant Synthetic Biology.

1 Instituto de Biologia Molecular y Celular de Plantas
Plant physiology (Impact Factor: 7.39). 05/2013; 162. DOI: 10.1104/pp.113.217661
Source: PubMed

ABSTRACT Plant Synthetic Biology aims to apply engineering principles to plant genetic design. One strategic requirement of Plant Synthetic Biology is the adoption of common standardized technologies that facilitate the construction of increasingly complex multigene structures at the DNA level while enabling the exchange of genetic building blocks among plant bioengineers. Here we describe GoldenBraid2.0 (GB2.0), a comprehensive technological framework that aims to foster the exchange of standard DNA parts for Plant Synthetic Biology. GB2.0 relies on the use of TypeIIS restriction enzymes for DNA assembly and proposes a modular cloning schema with positional notation that resembles the grammar of natural languages. Apart from providing an optimized cloning strategy that generates fully exchangeable genetic elements for multigene engineering, the GB2.0 toolkit offers an ever-growing open collection of DNA parts, including a group of functionally-tested, pre-made genetic modules to build frequently-used modules like constitutive and inducible expression cassettes, endogenous gene silencing and protein-protein interaction tools, etc. Use of the GB2.0 framework is facilitated by a number of web resources which include a publicly available database, tutorials and a software package that provides in silico simulations and lab protocols for GB2.0 part domestication and multigene engineering. In short, GB2.0 provides a framework to exchange both information and physical DNA elements among bioengineers to help implement Plant Synthetic Biology projects.

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Available from: Jose Miguel Blanca, Sep 03, 2015
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    • "A vector harbouring large DNA molecules with changeable elements provides an opportunity to efficiently deliver multiple genes. For example, the development of multigene vector systems, such as GoldenGate, ePathBricks and BioBrick, allows assembly of several genes in a predictable manner (Appleton et al., 2014; DePaoli et al., 2014; Liu et al., 2013; Sarrion-Perdigones et al., 2013, 2014; Xu and Koffas, 2013). By combining de novo DNA synthesis with seamless assembly methods, it is now feasible to construct multigene vectors (Kosuri and Church, 2014). "
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