Isolation and manipulation of mammalian neural stem cells in vitro

Department of Molecular Embryology, Max Planck Institute of Immunobiology, Freiburg, Germany.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2009; 482:143-58. DOI: 10.1007/978-1-59745-060-7_9
Source: PubMed

ABSTRACT Neural stem cells are potentially a source of cells not only for replacement therapy but also as drug vectors, bringing bioactive molecules into the brain. Stem cell-like cells can be isolated readily from the human brain, thus, it is important to find culture systems that enable expansion in a multipotent state to generate cells that are of potential use for therapy. Currently, two systems have been described for the maintenance and expansion of multipotent progenitors, an adhesive substrate bound and the neurosphere culture. Both systems have pros and cons, but the neurosphere may be able to simulate the three-dimensional environment of the niche in which the cells reside in vivo. Thus, the neurosphere, when used and cultured appropriately, can expand and provide important information about the mechanisms that potentially control neural stem cells in vivo.

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    • "Neurospheres were first described in 1992, using both adult and the embryonic CNS (Reynolds and Weiss, 1992; Reynolds et al., 1992), and are defined as three dimensional structures that grow as floating aggregates and are composed by a heterogeneous mixture of multipotent NSC, proliferating neural progenitor cells (NPC) and postmitotic neurons and glia (Jensen and Parmar, 2006). The neurosphere system has been used extensively since it is thought to provide a three-dimensional environment that can mimic the neurogenic niche (Giachino et al., 2009). In fact, the neurosphere system is considered as a valuable in vitro model to study neurodevelopmental processes, as it is maintained under defined 0736-5748/© 2014 ISDN. "
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