Recombinant bovine growth hormone identification and the kinetic of elimination in rainbow trout treated by LC-MS/MS
ABSTRACT The efficiency of the administration of recombinant bovine growth hormone (rbGH) to enhance fish growth has been widely reported in the literature. Although its use is probable and has been described in several countries, rbGH is prohibited in the European Union (Council Decisions 1994/936/EC , 1999/879/EC ). In this context, an analytical strategy was optimised in order to identify rbGH-treated fish. Currently, one of the most difficult challenges for the detection of rbGH in fish is probably the choice of the matrix and the corresponding available quantity for analysis. Therefore, based on a previous efficient protocol developed for mammalian species, a method was adapted for very limited serum volume (50 µl) and was successfully implemented to analyse serum collected from seven trout treated with rbGH. The detection of rbGH was possible from the very first day after administration and the hormone could easily be identified at least for 1 month with levels in the range 5-10 µg ml(-1). The limits of detection (LODs) estimated around 0.5 µg ml(-1) rbGH in fish serum are far below observed concentrations in incurred samples and therefore attest to the relevance of the developed protocol.
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ABSTRACT: Recombinant bovine growth hormone (rbST) is produced in large quantities and widely used in a number of countries worldwide to stimulate milk production and as a general growth promoter in meat producing animals. The use of this compound in animal production is strictly regulated by food safety directives in force, in particular in the EU. The control of its potential misuse constitutes an analytical challenge which many laboratories have attempted to tackle over the last 15 years. Several strategies can be considered to demonstrate somatotropin treatment to animals either based on biomarkers monitoring (IGF-I, antibodies produced by the animal after treatment...) or directed to the detection of the rbST itself by LC-MS after specific sample preparation. Recently, the challenge of rbST monitoring in blood of several animals species (bovine, goat) has been overcome and current research work focuses on other matrices such as milk or other animal species of interest such as fish. Successful protocols have been implemented thanks to the development of efficient purification procedure combined with the use of last MS generation instruments and selective acquisition modes. In parallel to these approaches, more global and untargeted strategies such as metabolomics have also proven their relevance for screening purposes. As the Joint FAO/WHO Expert Committee on Food Additives (JECFA) has scheduled to re-evaluate rbST during its next meeting in November 2013 and launched a call for data in January 2013, the present review article proposes an overview of the state of the art in the detection of rbST treated animals.TrAC Trends in Analytical Chemistry 10/2013; DOI:10.1016/j.trac.2013.08.006 · 6.61 Impact Factor