Fungus-Mediated Synthesis of Gold Nanoparticles: A Novel Biological Approach to Nanoparticle Synthesis

Mazandaran University of Medical Sciences, School of Pharmacy, Sari 48185861, Iran.
Journal of Nanoscience and Nanotechnology (Impact Factor: 1.56). 02/2013; 13(2):1427-30. DOI: 10.1166/jnn.2013.5989
Source: PubMed


The biological effects of nanoparticles and their uses as molecular probes are research areas of growing interest. The present study demonstrates an eco-friendly biosynthesis of gold nanoparticles. The pure colonies of penicillium aurantiogriseum, penicillium citrinum, and penicillium waksmanii were cultured in fluid czapek dox broth. Then, their supernatants were examined for the ability to produce gold nanoparticles. In this step, 1 mM solution of AuCl added to the reaction matrixes separately. The reactions were performed in a dark environment at 28 degrees C. After 24 hours, it was observed that the color of the solutions turned to dark purple from light yellow. Synthesized gold nanoparticles were characterized by using UV-Visible Spectroscopy, Nano Zeta Sizer, Scanning Electron Microscopy and Fourier transformed infrared spectroscopy. The results showed that the gold nanoparticles were formed fairly uniform with spherical shape with the Z-average diameter of 153.3 nm, 172 nm and 160.1 nm for penicillium aurantiogriseum, penicillium citrinum, and penicillium waksmanii, respectively. The Fourier transformed infrared spectra revealed the presence of different functional groups to gold nanoparticles which were present in the fungal extract. The current approach suggests that the rapid synthesis of nanoparticles would be proper for developing a biological process for mass scale production.

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    • "The control sample (without gold ions) showed no change in color when incubated under same conditions. The UV-Vis spectrum exhibited an absorption band at around 527 nm (Figure 1A), which is a typical plasmon band for gold nanoparticles (Honary et al., 2013a). The UV-vis Spectrum of supernatant withought gold nanoparticles in low wavelength region, recorded from the reaction medium, exhibited an absorption band around 265 nm and it was attributed to aromatic amino acids of proteins (Figure 1B). "
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    • "Fusarium oxysporum produced spherical and triangular morphologies of particles with a size range of 20 to 40 nm [15]. Honary et al. [57] reported that Penicillium aurantiogriseum, Penicillium citrinum, and Penicillium waksmanii synthesized AuNPs that were fairly uniform with spherical shapes and had average diameters of 153.3, 172, and 160.1 nm, respectively. Alternatively, the fungi Aspergillus fumigates[30] and Neurospora crassa[36] produced average AuNP sizes of 25 and 32 nm, respectively. "
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