Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis--a systematic review.

Federal University of Goiás, Goiânia, GO, Brazil.
Journal of applied oral science: revista FOB (Impact Factor: 0.8). 11/2008; 16(6):364-8. DOI: 10.1590/S1678-77572008000600002
Source: PubMed

ABSTRACT The efficacy of the sodium hypochlorite (NaOCl) and chlorhexidine (CHX) on Enterococcus faecalis was evaluated by systematic review and meta-analysis. The search strategies included search in electronic biomedical journal databases (MEDLINE, EMBASE, CENTRAL) and handsearching records, using different matches of keywords for NaOCl, CHX and Enterococcus faecalis. From 41 in vivo studies, 5 studies met the inclusion criteria. In a sample containing 159 teeth, E. faecalis was detected initially in 16 (10%) teeth by polymerase chain reaction (PCR) and 42 (26.4%) teeth by microbial culture techniques. After root canal disinfection, this species was observed in 11 (6.9%) teeth by PCR and 12 (7.5%) teeth by culture. Risk differences of included studies were combined as generic inverse variance data type (Review Manager Version 5.0 _ Cochrane Collaboration,, accessed 15 May 2008), taking into account the separate tracking of positive and negative cultures/PCR. The level of statistical significance was set at p<0.05. In conclusion, NaOCl or CHX showed low ability to eliminate E. faecalis when evaluated by either PCR or culture techniques.


Available from: Cláudio Rodrigues Leles, Feb 18, 2014
1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: ZET Pulpal ve periapikal inflamasyonun gelişmesinde ve devam etmesinde mikroorganizmalar büyük rol oynamaktadır. Kök kanal tedavisinin başarı-sı, kök kanal sisteminden mikrobiyal kontaminasyonun tamamen elimine edilmesine bağlıdır. Kök kanallarının mekanik preparasyonuyla bakteriyel popülasyonda düşüş olmasına rağmen, antibakteriyel irriganlar ve ilaçlar kullanılmaksızın bakterilerin elimine edilmesinde tam bir başarı sağlanama-maktadır. Bu makalede bilinen ve gelişmekte olan kök kanal irriganlarının antimikrobiyal etkileri güncel literatür bilgileri ışığı altında gözden geçiril-miştir. Anahtar kelimeler: Antimikrobiyal etki, kök kanal irriganları, kök kanal te-davisi SUMMARY Current approach to root canal irrigants Microorganisms have an important role in the development and continua-tion of pulpal and periapical inflammation. The success of root canal treat-ment depends on the exclusive elimination of microbial contamination from the root canal system. Although mechanical instrumentation of root canals provide a decrease in bacterial population, a complete success in bacterial elimination cannot be achieved without the use of antimicrobial irrigants and drugs. In this paper antimicrobial effects of the root canal irrigants which are in use or under investigation are reviewed based on the current literature.
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study evaluated the dentin bond strength (BS) and the antibacterial activity (AA) of six adhesives against strict anaerobic and facultative bacteria. Three adhesives containing antibacterial components (Gluma 2Bond (glutaraldehyde)/G2B, Clearfil SE Protect (MDPB)/CSP and Peak Universal Bond (PUB)/chlorhexidine) and the same adhesive versions without antibacterial agents (Gluma Comfort Bond/GCB, Clearfil SE Bond/CSB and Peak LC Bond/PLB) were tested. The AA of adhesives and control groups were evaluated by direct contact method against four strict anaerobic and four facultative bacteria. After incubation, according to the appropriate periods of time for each microorganism, the time to kill microorganisms was measured. For BS, the adhesives were applied according to manufacturers' recommendations and teeth restored with composite. Teeth (n=10) were sectioned to obtain bonded beams specimens, which were tested after artificial saliva storage for one week and one year. BS data were analyzed using two-way ANOVA and Tukey test. Saliva storage for one year reduces the BS only for GCB. In general G2B and GCB required at least 24hours for killing microorganisms. PUB and PLB killed only strict anaerobic microorganisms after 24hours. For CSP the average time to eliminate the Streptococcus mutans and strict anaerobic oral pathogens was 30minutes. CSB showed no AA against facultative bacteria, but had AA against some strict anaerobic microorganisms. Storage time had no effect on the BS for most of adhesives. The time required to kill bacteria depended on the type of adhesive and never was less than 10minutes. Most of adhesives showed stable bond strength after one year and the Clearfil SE Protect may be a good alternative in restorative procedures performed on dentin, considering its adequate bond strength and better antibacterial activity. Copyright © 2015. Published by Elsevier Ltd.
    Journal of Dentistry 01/2015; 43(4). DOI:10.1016/j.jdent.2015.01.004 · 2.84 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC(®) 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by (13)C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.
    Brazilian Journal of Microbiology 01/2014; 45(3):769-79. DOI:10.1590/S1517-83822014000300002 · 0.45 Impact Factor