Article

The Sall2 transcription factor is a novel p75NTR binding protein that promotes the development and function of neurons

The Department of Surgery and the Helen Diller Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA 94115, USA.
Annals of the New York Academy of Sciences (Impact Factor: 4.31). 12/2008; 1144:53-5. DOI: 10.1196/annals.1418.026
Source: PubMed

ABSTRACT Nerve growth factor (NGF) regulates the functions of neurons by binding the p75 neurotrophin receptor (p75NTR) and TrkA receptors. It is shown here that it has been found that the Sall2 transcription factor forms a complex with p75NTR. NGF dissociates Sall2 from p75NTR and also activates TrkA, which promotes translocation of Sall2 into the nucleus. Nuclear Sall2 activates target genes that modulate the growth and development of neurons. Thus, one way that p75NTR and TrkA can modulate the functions of neurons is by regulating the localization and actions of Sall2.

0 Followers
 · 
112 Views
  • Source
    • "Indeed, different cell lines often exhibit varied patterns of expression of stem cell pluripotency markers during differentiation (Abeyta et al., 2004; Hailesellasse Sene et al., 2007). Other notable genes significantly up-regulated at day 3 include genes that are implicated in nervous system development such as Sall2 (Pincheira and Donner, 2008), Zic2 (Salero and Hatten, 2007), Pou3f1 (Ryu et al., 2007), Lpar4 (Shin et al., 2007), Sox11 (Uwanogho et al., 1995), Sort1 (Jansen et al., 2007) and Slc7a3, a brain specific cationic transporter (Hosokawa et al., 1997). Among them, Sall2, Zic2, Pou3f1 and Sox11 are transcription factors that promote the development and function of neurons. "
    Gene Expression Patterns 04/2011; · 1.36 Impact Factor
  • Source
    • "Indeed, different cell lines often exhibit varied patterns of expression of stem cell pluripotency markers during differentiation (Abeyta et al., 2004; Hailesellasse Sene et al., 2007). Other notable genes significantly up-regulated at day 3 include genes that are implicated in nervous system development such as Sall2 (Pincheira and Donner, 2008), Zic2 (Salero and Hatten, 2007), Pou3f1 (Ryu et al., 2007), Lpar4 (Shin et al., 2007), Sox11 (Uwanogho et al., 1995), Sort1 (Jansen et al., 2007) and Slc7a3, a brain specific cationic transporter (Hosokawa et al., 1997). Among them, Sall2, Zic2, Pou3f1 and Sox11 are transcription factors that promote the development and function of neurons. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Embryonic stem (ES) cells can be induced to differentiate into embryoid bodies (EBs) in a synchronised manner when plated at a fixed density in hanging drops. This differentiation procedure mimics post-implantation development in mouse embryos and also serves as the starting point of protocols used in differentiation of stem cells into various lineages. Currently, little is known about the potential influence of microRNAs (miRNAs) on mRNA expression patterns during EB formation. We have measured mRNA and miRNA expression in developing EBs plated in hanging drops until day 3, when discrete structural changes occur involving their differentiation into three germ layers. We observe significant alterations in mRNA and miRNA expression profiles during this early developmental time frame, in particular of genes involved in germ layer formation, stem cell pluripotency and nervous system development. Computational target prediction using Pictar, TargetScan and miRBase Targets reveals an enrichment of binding sites corresponding to differentially and highly expressed miRNAs in stem cell pluripotency genes and a neuroectodermal marker, Nes. We also find that members of let-7 family are significantly down-regulated at day 3 and the corresponding up-regulated genes are enriched in let-7 seed sequences. These results depict how miRNA expression changes may affect the expression of mRNAs involved in EB formation on a genome-wide scale. Understanding the regulatory effects of miRNAs during EB formation may enable more efficient derivation of different cell types in culture.
    Gene Expression Patterns 03/2011; 11(5-6):334-44. DOI:10.1016/j.gep.2011.03.004 · 1.36 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this paper, we propose the fabrication of an MIS type field emitter using single crystal Al2O3 insulator and Al electrode on an n-type Si(111) substrate.
Show more