Germination and seedling growth in grasspea (Lathyrus sativus) cultivars under salinity conditions.

Department of Agronomy, Faculty of Agriculture, Tarbiat Modarres University, P.O. Box 14115-336, Tehran, Iran.
Pakistan Journal of Biological Sciences 02/2007; 10(2):273-9. DOI: 10.3923/pjbs.2007.273.279
Source: PubMed

ABSTRACT In four grasspea varieties include ardabil, sharekord, mashhad and zanjan, the effects of different salinity concentrations on seed germination percent, proline concentration, malondialdehyde (MDA), germination index, radicle and hypocotyl length and weight were studied. Result showed that salinity had significant effects on seed germination percentage and germination index. The most and least of germination percentage were observed in 6 and 18 dS m(-1), respectively. Salinity had significantly effect on radicle and hypocotyl length, dry and fresh weight, MDA and proline concentration of seedlings. Salinity had not effect on dry weight of seedling. Increasing salinity reduced radicle and hypocotyl length, dry and fresh weight of seedlings and enhanced proline and malondialdehyde in them. Sharkord cultivar had the most germination percentage at 18 dS m(-1) sodium chloride. Sharkord and ardabil varieties were the most tolerance and sensitive varieties to salinity stress, respectively.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Vicia sativa L., an annual winter growing leguminous plant, is a valuable source of protein and minerals for cattle. It was tested for salinity tolerance in laboratory and pot experiments using three levels of salinity viz., 5, 10, and 15dSm -1 . It was observed that the germination and seedling growth declined at 10 and 15dSm -1 level of applied salt. The survival of seedling was affected severely in high salt concentration. Similarly various growth parameters including degree of branching, length of shoots and roots, fresh weight of shoots and roots declined at 10, and 15dSm -1 salinity levels. The present findings suggest that Vicia sativa can tolerate moderate levels of salinity and might be cultivated on marginal saline habitat as a source of fodder and nutrients to the soil.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Response of six improved grass pea genotypes to prolonged salinity stress was investigated on seedlings grown in pot experiment using 150 mM NaCl up to 60 days of growth after commencement of treatment (DAC). NaCl exposure significantly reduced growth potential of varieties PUSA-90-2 and WBK-CB-14, but no such effect was observed in varieties B1, BioL-212 and in two mutant lines LR3 and LR4. A time-bound measurement at 15, 30 and 60 DAC revealed significant reduction in plant dry matter production, orchestrated through abnormally low capacity of leaf photosynthesis accompanied by low K + /Na + ratio and onset of oxidative stress in all six genotypes at 15 DAC and the extension of the phenomena in PUSA-90-2 and WBK-CB-14 to 60 DAC. High superoxide dismutase (SOD) activity coupled with low ascorbate redox and declining ascorbate peroxidase (APX) and catalases (CAT) levels led to abnormal rise in H 2 O 2 content at reproductive stage (30 DAC) in the latter two genotypes, consequently, resulting in NaCl-induced oxidative damage. H 2 O 2 level in the rest of the four genotypes was modulated in a controlled way by balanced action of SOD, APX and CAT, preventing oxidative damage even under prolonged NaCl-exposure. Enzyme isoforms were involved in regulation of foliar H 2 O 2 -metabolism, which was critical in determining As tolerance of grass pea genotypes.
    ISRN Agronomy. 12/2013; 2013.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: High salt level of a germination medium may induce a reduction, delay and even complete inhibition of germination due to osmotic effect and/or ion toxicity. The objective of this study was to investigate the effect of salinity due to NaCl on germination and early seedling growth of two crops, Pisum sativum var. abyssinicum and Lathyrus sativus. Seeds of these two crops were treated with NaCl induced saline germinating media prepared in Petri dishes. Fifty (50) surface sterilized seeds per Petri dish were sown in five salt treatments (0, 5, 7, 9 and 15 dSm -). Each treatment was replicated four times. Germination percentage, shoot length and root length of both crops decreased with an increase in salinity level. Although both crops are low salt tolerant legumes, P. sativum var. abyssinium was found to be less tolerant than L. sativus. This study could be strengthened by further work under field conditions and also at mature vegetative and reproductive stages of the crops.