Quantitative Transcriptomics using Designed Primer-based Amplification.
ABSTRACT We developed a novel Designed Primer-based RNA-sequencing strategy (DP-seq) that uses a defined set of heptamer primers to amplify the majority of expressed transcripts from limiting amounts of mRNA, while preserving their relative abundance. Our strategy reproducibly yielded high levels of amplification from as low as 50 picograms of mRNA while offering a dynamic range of over five orders of magnitude in RNA concentrations. We also demonstrated the potential of DP-seq to selectively suppress the amplification of the highly expressing ribosomal transcripts by more than 70% in our sequencing library. Using lineage segregation in embryonic stem cell cultures as a model of early mammalian embryogenesis, DP-seq revealed novel sets of low abundant transcripts, some corresponding to the identity of cellular progeny before they arise, reflecting the specification of cell fate prior to actual germ layer segregation.
- SourceAvailable from: Agustin Rojas-Muñoz[Show abstract] [Hide abstract]
ABSTRACT: Time-dependent extracellular manipulations of human pluripotent stem cells can yield as much as 90% pure populations of cardiomyocytes. While the extracellular control of differentiation generally entails dynamic regulation of well-known pathways such as Wnt, BMP, and Nodal signaling, the underlying genetic networks are far more complex and are poorly understood. Notably, the identification of these networks holds promise for understanding heart disease and regeneration. The availability of genome-wide experimentation, such as RNA and DNA sequencing, as well as high throughput surveying with small molecule and small interfering RNA libraries, now enables us to map the genetic interactions underlying cardiac differentiation on a global scale. Initial studies demonstrate the complexity of the genetic regulation of cardiac differentiation, exposing unanticipated novel mechanisms. However, the large datasets generated tend to be overwhelming and systematic approaches are needed to process the vast amount of data to improve our mechanistic understanding of the complex biology. Systems biology methods spur high hopes for parsing vast amounts of data into genetic interaction models that can be verified experimentally and ultimately yield functional networks that expose the genetic connections underlying biological processes.For further resources related to this article, please visit the WIREs website.Conflict of interest: The authors have declared no conflicts of interest for this article.Wiley Interdisciplinary Reviews Systems Biology and Medicine 04/2014; · 3.68 Impact Factor
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ABSTRACT: Mouse epiblast stem cells (EpiSCs) display temporal differences in the upregulation of Mixl1 expression during the initial steps of in vitro differentiation, which can be correlated with their propensity for endoderm differentiation. EpiSCs that upregulated Mixl1 rapidly during differentiation responded robustly to both Activin A and Nodal in generating foregut endoderm and precursors of pancreatic and hepatic tissues. By contrast, EpiSCs that delayed Mixl1 upregulation responded less effectively to Nodal and showed an overall suboptimal outcome of directed differentiation. The enhancement in endoderm potency in Mixl1-early cells may be accounted for by a rapid exit from the progenitor state and the efficient response to the induction of differentiation by Nodal. EpiSCs that readily differentiate into the endoderm cells are marked by a distinctive expression fingerprint of transforming growth factor (TGF)-β signalling pathway genes and genes related to the endoderm lineage. Nodal appears to elicit responses that are associated with transition to a mesenchymal phenotype, whereas Activin A promotes gene expression associated with maintenance of an epithelial phenotype. We postulate that the formation of definitive endoderm (DE) in embryoid bodies follows a similar process to germ layer formation from the epiblast, requiring an initial de-epithelialization event and subsequent re-epithelialization. Our results show that priming EpiSCs with the appropriate form of TGF-β signalling at the formative phase of endoderm differentiation impacts on the further progression into mature DE-derived lineages, and that this is influenced by the initial characteristics of the cell population. Our study also highlights that Activin A, which is commonly used as an in vitro surrogate for Nodal in differentiation protocols, does not elicit the same downstream effects as Nodal, and therefore may not effectively mimic events that take place in the mouse embryo.Philosophical Transactions of The Royal Society B Biological Sciences 12/2014; 369(1657). · 6.31 Impact Factor
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ABSTRACT: Abstract A significant challenge to the effective application of RNA-seq to the complete transcript analysis of low quantity and/or degraded samples is the amplification of minimal input RNA to enable sequencing library construction. Several strategies have been commercialized in order to facilitate this goal. However, each strategy has its own specific protocols and methodology, and each may introduce unique bias and in some cases show specific preference for a collection of sequences. Our wider investigation of human spermatozoal RNAs was able to reveal their complexity despite being generally characterized by low quantity and high fragmentation. In this study, the following four commercially available RNA-seq amplification and library protocols for the preparation of low quantity/highly fragmented samples, SMARTer™ Ultra Low RNA (SU) for Illumina® Sequencing, SeqPlex RNA Amplification (SP), Ovation® RNA-Seq System V2 (OR), and Ovation® RNA-Seq Formalin Fixed Paraffin Embedded System (FFPES) were assessed using human sperm RNAs. Further investigation analyzed the effects on the end results of two different library preparation methods, Encore NGS Multiplex System I (Enc) and Ovation Ultralow Library Systems (UL), that appeared best suited to this type of RNA, along with other potential confounding factors such as FFPE preservation. Our results indicate that for each library preparation protocol, the differences in the initial amount of input RNA and choice of RNA purification step do not generate marked differences in terms of RNA profiling. However, substantial disparity is introduced by individual amplification methods prior to library construction. These significant differences may be caused by the different priming methods or amplification strategies used in each of the four different protocols examined. The observation of intra-sample variation introduced by the choice of protocol highlights the role that external factors play in planning and subsequent reliable interpretation of results of any RNA-seq experiment.Systems Biology in Reproductive Medicine 07/2014; · 1.70 Impact Factor
Quantitative Transcriptomics using
Designed Primer-based Amplification
Vipul Bhargava1, Pang Ko2, Erik Willems3, Mark Mercola2,3& Shankar Subramaniam1,2,4
1Bioinformatics and Systems Biology Graduate Program, University of California at San Diego, La Jolla, California, USA,
2Department of Bioengineering, University of California at San Diego, La Jolla, California, USA,3Sanford-Burnham Medical
Research Institute, La Jolla, California, USA,4Departments of Cellular and Molecular Medicine and Chemistry and Biochemistry,
University of California at San Diego, La Jolla, California, USA.
We developed a novel Designed Primer-based RNA-sequencing strategy (DP-seq) that uses a defined set of
heptamer primers to amplify the majority of expressed transcripts from limiting amounts of mRNA, while
preserving their relative abundance. Our strategy reproducibly yielded high levels of amplification from as
low as 50 picograms of mRNA while offering a dynamic range of over five orders of magnitude in RNA
concentrations. We also demonstrated the potential of DP-seq to selectively suppress the amplification of
the highly expressing ribosomal transcripts by more than 70% in our sequencing library. Using lineage
novel sets of low abundant transcripts, some corresponding to the identity of cellular progeny before they
arise, reflecting the specification of cell fate prior to actual germ layer segregation.
specific probes to their corresponding targets, and thus result in analog expression profiles and a low dynamic
range. With the recent dramatic increase in sequencing depth and the decrease in cost per base sequenced, high
throughput sequencing technologies have emerged as preferred platforms for mRNA expression analysis of the
complex mammalian transcriptome5,6.
A major limitation of the current gold standard RNA sequencing approach7is the large amount of starting
or even for FACS sorted cell populations. Also, the standard RNA-seq protocol7maintains the relative order of
transcript expression with a few highly expressed transcripts occupying majority of the sequencing space. This
low abundant transcripts within large mammalian transcriptomes is further hampered by multireads and biases
introduced by the transcript length10and random hexamer primer hybridization11. A number of amplification-
based protocols have been developed to address these issues such as ‘‘random priming’’ strategies12–14, which
utilize the hybridization and extension potential of hexamer/heptamer primers to amplify the starting material
to mis-hybridization of primers and/or primer dimerization. Furthermore, these methods do not discriminate
the regions of the transcriptome to amplify, a feature also shared by other uniform amplification based
Here we describe a novel quantitative cDNA expression profiling strategy, involving the amplification of a
majority of the mouse transcriptome using a defined set of 44 heptamer primers. The amplification protocol
allows an efficient amplification of the majority of the expressed transcripts from as low as 50 pg of mRNA and
was optimized to reduce mis-hybridization of primers and primer dimerization. We further explored the poten-
as ribosome encoding transcripts. Our sequencing data demonstrated a significant reduction in the representa-
length cDNA amplification strategy (Smart-seq)19and observed comparable transcriptome coverage and similar
technical noise. We implemented DP-seq on a model of embryological lineage segregation, achieved by graded
activation of Activin A/TGFb signaling in mouse embryonic stem cells (mESCs). The fold changes in transcript
ext Generation Sequencing-based approaches for whole transcriptome analysis produce millions of
sequencing reads, which represent the vast majority of the expressed transcripts. The high number of
reads allows a digital estimation of transcript abundance, resulting in a large dynamic range and high
GENOME-WIDE ANALYSIS OF
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SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
expression were in excellent agreement with quantitative RT-PCR
and we observed a dynamic range spanning more than five orders of
magnitude in RNA concentration with a reliable estimation of low
markers, while the high sensitivity indicated that novel lineage spe-
cific transcripts anticipate the differentiation of specific cell types.
Sequencing-library generation using heptamer primers based
amplification. A novel cDNA sequencing-library generation
methodology was developed to reliably represent the relative
abundance of transcripts using limited amounts of mRNA. DP-seq
consisted of three distinct phases (Figure 1a). In the first phase, we
developed a primer design strategy that identified a defined set of 44
heptamer primers amplifying . 80% of the mouse transcriptome
(Figure 1a, green panel). This strategy incorporated known biases in
PCR, namely the secondary structure of primer-binding sites in
single stranded cDNA, GC content and the proximity to the 39 end
of the transcript to identify potential primer-binding sites. Of the
16384 input sequences of heptamer primers, we selected primers
with annealing temperatures between 16–25uC. To minimize mis-
priming, heptamer primers starting with adenines at the 59 end and
purine rich primers were filtered out. Next, an iterative randomized
algorithm was implemented to identify 44 heptamer primers, which
preferentially amplified unique regions of mouse transcripts (see
Supplementary Fig. S1 online). The primers were split into
multiple sets ensuring no two primers had a mutual interaction
energy (Gibbs free energy) lesser than -5 kcal/mol in order to
reduce primer dimerization. Of the 26566 known transcripts in the
15072 (56.7%) transcripts uniquely.
In the second phase of the methodology, we performed a targeted
amplification of the mouse transcriptome using the defined set of
heptamer primers (Figure 1a, pink panel). This phase consisted of
two components; (i) determination of the minimum length of the
primer required to achieve efficient amplification and (ii) optimiza-
tion of the amplification protocol to extend and amplify partially
hybridized primers. We determined 14 bp (Tm, 45–50uC) as the
optimal length of the primers required to efficiently amplify regions
of interest in the mouse transcriptome. As such the heptamer
Figure 1 | Schematicrepresentationofsequencinglibrarypreparationusingheptamerprimersbasedamplification,DP-seq. (a)Step1:Primerselection
was based on identifying potential primer-binding sites that were less likely to form secondary structures and resided upstream to the unique regions on
to the single stranded cDNA library and were extended and amplified as indicated. Step 3: Library preparation. Illumina paired end adaptors were ligated
to the ends of the amplicon library and the correct orientation of adaptors were selected. The library was further amplified using Illumina’s paired end
adaptor primers and were size selected for synthesis-based sequencing. (b) Expression profiles of genes responding to graded activation of the Activin A/
TGFb signaling pathway in mouse embryoid bodies at day 4. Quantitative RT-PCR data was normalized with respect to untreated serum-free media
controls. (c) The fidelity of amplification of the cDNA library using heptamer primers. Fold changes observed in 11 genes (from part (b), Afp and Cer1)
across different dosages of Activin A showed perfect agreement with quantitative RT-PCR performed on cDNA (R25 0.94; n 5 45). (d) Distribution of
reads on the mouse genome.
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
primers were extended by addition of a universal 7 bp sequence (59-
CCGAATA9-39) at the 59 end of heptamer primers. Standard PCR
protocols failed to amplify partially hybridized primers because of
low annealing temperatures of the last 7 bp, resulting in significant
distortions in the expression level of low abundance transcripts. We
therefore developed a novel protocol that uses a combination of
mesophilic (Klenow polymerase) and thermophilic polymerases
(Taq polymerase) to efficiently amplify regions of interest on
cDNA. Klenow polymerase, which retains its optimal extension
activity at 37uC, extends our partially hybridized primers (last
7 bp) at this temperature. The extended primers withstand the high
ing in the formation of a double stranded amplicon library. These
amplicons possess complementary sequences of the entire 14 bp of
50uC), they efficiently hybridize to the template and allowamplifica-
tion of these amplicons during the subsequent cycles of Taq poly-
In the last phase of the sequencing library generation, the ampli-
con library was 59 end phosphorylated and ligated to Illumina’s
adaptors(Figure1a,bluepanel). Sinceonlydistinctadaptor orienta-
tion fragments can be sequenced in Illumina’s platform, we used a
biotin-streptavidin chemistry (see Supplementary Methods online)
toselectthecorrectorientationsof theadaptors.Thefragments were
later PCR amplified using Illumina’s adaptor specific PCR primers
and size selected for synthesis-based sequencing. The selection of
fragments with a correct orientation of the adaptors can be skipped
using a custom sequencing primer that contains the universal tail
sequence (59-CCGAATA9-39) at its 39 end.
Evaluation of heptamer amplification-based transcriptomics. We
implemented DP-seq on an in vitro cell culture based model of
primitive streak (PS) induction in mESCs20,21. Signaling by the
TGFb-family member Nodal through Activin receptor like kinase-
dose-dependent induction of these tissues can be mimicked by
treatment with Activin A. Various dosages of Activin A (3 ng/mL,
AA3;15 ng/mL,AA15;and100 ng/mL,AA100)werethereforeused
a small molecule inhibitor, SB-431542 (SB)27, was used to induce
As expected, small doses of Activin A substantially induced meso-
dermal markers (e.g., Kdr, Mesp1) while higher doses of Activin A
were required for the induction of anterior lineages including defin-
itive endoderm (e.g., Gsc, Foxa2) (Figure 1b). On the other hand,
complete inhibition of Activin A/TGFb signaling caused an up-
regulation of neuro-ectoderm markers (e.g., Sox1)29. Moreover,
direct target genes (e.g., Lefty1, Lefty2 and T also known as
Brachyury)30,31of the Activin A/TGFb signaling pathway were regu-
lated dose dependently. The differential expression of these low
abundant genes in DP-seq showed excellent concordance with
quantitative RT-PCR (R25 0.94, Figure 1c) thus validating the
For a typical transcriptome measurement, we obtained ,30 mil-
lion reads per lane of Illumina’s genome analyzer flow cell (Table 1).
About 59% (18 million) reads uniquely mapped to more than 11000
transcripts with $10 reads. About 19% of the reads were non-
uniquely mapped with a vast majority of them mapping to isoform
groups. Another 18% of the reads (71% uniquely) mapped to geno-
mic locations (excluding the open reading frames of known tran-
scripts) and mitochondria transcripts (Figure 1d). Of these genomic
reads, 72% mapped to intronic regions of transcripts while another
likely represent non-coding RNA, since we did not see a strong
correlation between the fold changes in intronic reads with those
from proximal exons.
The experimental data indicated expression of more than 100,000
different primer-binding sites representing ,18,000 known tran-
by DP-seq. On average, we obtained expression of 10 different pri-
mer-binding sites for each expressed transcript. Notably, each site
provided an independent measurement of relative abundance ser-
ving as technical replicates for the experiment (see Supplementary
Fig. S2 online).
priming or single nucleotide polymorphisms (SNPs) in the primer-
binding sites. Fold changes observed in predicted and mis-primed
binding sites were highly correlated (R25 0.88) suggesting that mis-
primed PCR products were able to conserve the relative abundance
of transcripts (see Supplementary Fig. S2 online). Mis-primed pro-
ducts were mainly stabilized by a favorable interaction between the
3’) and the upstream regions of the primer-binding sites (tail inter-
action, see Supplementary Fig. S3 online). Finally, we observed no
indication of primer – dimerization.
Analysis ofthetechnicalreplicates revealedastrong correlationin
quantitative transcript expression (R25 0.96, Figure 2a). To assess
the dynamic range, we spiked the untreated control (serum free
media, SFM) with six artificial transcripts of the yeast POT1 pro-
moter (,180 bp). The transcripts were flanked with different
Table 1 | Mapping summary of the sequencing experiment. Reads were first aligned to the NCBI mRNA RefSeq database allowing up to 2
mismatches. Unmapped reads were later aligned to the mouse genome including mitochondria. Multireads refer to reads that mapped to
more than one transcript/genomic locations. TR refers to technical replicates
(15 ng/mL) TR1
(15 ng/mL) TR2
Unique reads (mRNA Refseq)
Multireads (Isoform group only, mRNA Refseq)
Multireads (mRNA refseq)
Genomic and Mitochondria
Transcripts (Unique reads .5 10)
Transcripts (Multireads .5 10)
Binding Sites (Unique reads .5 10)
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
heptamer primer-binding sites and mixed in different dilutions,
most abundant transcript was similar in expression with the b-actin
abundance inour biologicalsamples.Ourprimerswere abletoeffec-
tively amplify all the six transcripts and maintained their relative
abundance (R25 0.99, Figure 2b). The distribution of fold changes
(see Supplementary Fig. S2 online) observed in all possible pairwise
comparisons of the samples was broad (228–210) suggesting a much
higher dynamic range in comparison to microarray platforms (few
We next prepared serial dilutions of mRNA from 10 ng to 1 pg
sequencing library. The number of amplification cycles was
increased for lower dilutions to achieve appropriate amounts of
DNA for the library construction. The transcript measurements
from the technical replicates consistently showed high correlations
for the libraries prepared from 10 ng–50 pg of mRNA (Figure 2c).
The transcriptome coverage remained high even for libraries pre-
pared from 1 pg of mRNA (, 6000 transcripts; see Supplementary
Table S1 online), although the noise in the quantification of the
transcripts increased substantially (see Supplementary Fig. S4
online). We further investigated whether the transcript measure-
ments were conserved within the dilution series of mRNA. Global
transcript measurements of libraries constructed from at least 50 pg
of mRNA showed high correlation with the libraries constructed
from 10 ng of mRNA (see Supplementary Fig. S5 online).
Sequencing libraries constructed from 1 pg of mRNA showed sig-
nificant deviations in measurements of low copy number transcripts
from 10 ng of libraries and a considerable amount of spurious PCR
artifacts were observed.
cDNA amplification (see Supplementary Fig. S3 online). The most
dominant bias came from local secondary structures of the single
stranded cDNA. Regions with stable secondary structures prevented
the heptamer primer-binding sites from hybridizing with their cor-
responding heptamer primers, resulting in their poor representation
in the sequencing library. There was also an inherent bias towards
preferential amplification of fragments with shorter lengths and
lower GC content, which are known to be associated with Taq
Polymerase amplification and have been reported in other multi-
plexed PCR strategies32. Finally, we observed that the majority of
the experimental heptamer primer-binding sites resided in prox-
imity to the 3’ end of the transcripts mainly because of the inability
of the reverse transcriptase to produce full-length cDNA.
To determine whether DP-seq is capturing the majority of the
expressed transcripts, we performed standard RNA-seq (Std. RNA-
seq) on the AA3 sample using the protocol adopted from Mortazavi
et al., 20087. We observed comparable transcriptome coverage with
DP-seq libraries, representing . 80% of the expressed transcripts.
Analysis of the technical replicates obtained from DP-seq and Std.
RNA-seq revealed a similar noise structure (see Supplementary Fig.
S6 online). The PCR biases observed in our methodology distorted
the order of transcript expression within a biological sample (see
Supplementary Fig. S6 online) resulting in a similar or enriched
representation of the majority of low expressed transcripts (Reads
Per Kilobase per Million mapped reads (RPKM) #10 in the Std.
across different biological samples was not affected (shown in
Figure 1c) as these biases are expected to be similar for a given
transcript across different biological samples. Furthermore, we
observed an overlapping distribution of unique reads for the tran-
scripts encoding transcription factors (http://genome.gsc.riken.jp/
TFdb/) between the two protocols (see Supplementary Fig. S7
We then investigated a novel aspect of our primer design strategy
where we incorporated the PCR biases observed in our protocol to
suppress the representation of highly expressed ribosomal tran-
scripts, while maintaining the overall transcriptome coverage.
Transcripts encoding 81 ribosomal proteins occupied about 9% of
the sequencing space in the Std. RNA-seq library prepared from the
AA3 sample. Detailed analysis of the PCR biases led us to propose
heuristics on favorable amplification by our heptamer primers.
Amplicons with heptamer primer-binding sites in open configura-
tion (,24 Kcal/mol); significant tail interaction (.5 2 bp inter-
action between the last four bases of the universal tail and the cDNA
template); low GC content (, 0.55) and short fragment lengths
(, 300 bp) were heavily penalized for the ribosomal transcripts.
We designed three different primer sets and generated sequencing
libraries from the AA3 sample. Our sequencing data revealed up to
70% reduction in the representation of the ribosomal transcripts
sets (Figure 2d). Furthermore, the overall distribution of the reads
coming from the transcription factor family also exhibited a similar
distribution for a representative primer set (see Supplementary Fig.
S7 online). This data demonstrates the potential of our designed
primer based strategy to preferentially suppress the representation
of the transcripts of interest (e.g. highly expressed transcripts) and
distinguishes it from other uniform amplification based strategies.
Comparison with a different PCR-based RNA-Seq method. We
performed a thorough comparison of our methodology with Smart-
seq19, which performs full-length cDNA amplification from limiting
amounts of mRNA. Sequencing libraries were generated from 50
picograms of mRNA derived from Activin A (3 ng/mL and
100 ng/mL) treated samples using DP-seq and Smart-seq. The
same samples were also used to generate Std. RNA-seq libraries
Figure 2 | Performance of DP-seq. (a) Comparison of two Activin A
15 ng/ml dosage replicates (R25 0.96). (b) Six in vitro synthesized
transcripts derived from the yeast POT1 promoter with a length of 180 bp
foldchange ofup to105(R250.99)incomparison tothe lowest abundant
transcript. (c) Sequencing libraries constructed from serial dilutions of
constructedfromat least50 pgofmRNA showedhighcorrelations (R2) in
global expression measurements with the libraries made from 10 ng of
mRNA. (d) Suppression of the ribosomal transcripts representation in the
sequencing library generated from three different primer sets. The global
transcriptome coverage remained high for all primer sets.
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
from 10 ng of mRNA. The libraries prepared from both methods
were highly reproducible and displayed strong correlations in the
expression measurements of the transcripts in the technical
replicates (see Supplementary Fig. S8 online). Both DP-seq and
Smart-seq exhibited similar transcriptome coverage (see Supple-
mentary Table S1 online) and overlapping noise in the quanti-
fication of the transcripts (Figure 3a). However, the transcriptome
coverage obtained in either method was significantly lower than that
of Std. RNA-seq libraries with the majority of low expressed
transcripts (average RPKM , 3 in the Std. RNA-seq library)
showing stochastic loss. Consequently, the distribution of unique
reads for the low expressed transcripts was shifted towards a low
read number (Figure 3b). A similar observation was made for
moderately expressed transcripts (average RPKM between 3 and
300 in the Std. RNA-seq library) with DP-seq and Smart-seq
libraries displaying an overlapping distribution of unique reads
(see Supplementary Fig. S9 online). Our mapping analysis
revealed a significant length bias in Smart-seq sequencing libraries
resulting from poor amplification of long cDNA species (. 4 Kb).
This was not observed with DP-seq as it performs amplification of
selected regions of cDNA irrespective of its length, thus resulting in
higher representation of a vast majority of the long transcripts
(. 77%; Figure 3c). Expression measurements of differentiating
mESCs treated with a higher dose of Activin A (100 ng/mL)
showed comparable up-regulation of mesendoderm markers
(Cer1, Lefty1, Lefty2, Foxa2, Gsc etc.) and down-regulation of
mesoderm and ectoderm genes, implying the conservation of the
biological context in the sequencing libraries prepared from 50 pg
of mRNA with either method (Figure 3d).
We next sought to compare the differential gene expression
observed in DP-seq, Smart-seq and Std. RNA-seq for the two
Activin A dosages. Differentially expressed transcripts were iden-
(see Supplementary Methods online). The null distribution for Std.
RNA-seq libraries showed little technical variation; as such a large
proportion of differentially expressed transcripts were identified.
Smart-seqandDP-seqidentified acomparable number ofdifferenti-
allyregulatedtranscripts (1414and1297respectively), howeveronly
a small proportion of them were common between the two methods
(see Supplementary Fig. S9 online). Pairwise comparison of these
methods with Std. RNA-seq revealed 56% overlap of the differenti-
ally expressed transcripts. Only 191 differentially regulated tran-
scripts (common set) were common in all three methods. We
found however that the differentially regulated transcripts that were
method-specific are low expressed and were prone to large noise as
these transcripts showed lower RPKM distributions as compared to
of the fold changes observed for the common set in DP-seq and
Smart-seq libraries showed strong correlations; however, they were
poorly correlated with the fold changes observed in Std. RNA-seq
libraries (R25 0.6456 for DP-seq and R25 0.5740 for Smart-seq).
This highlights the issues caused by the increased noise in the quan-
tification of low copy number transcript measurements, which is
further amplified when using low amounts of input material.
Graded activation of the Activin A/TGFb signaling pathway in
mESCs. Mouse ESCs were differentiated in serum-free conditions
in the presence of varying doses of Activin A and SB and the mRNA
was profiled at day 4 (equivalent to 6.5–7.5 dpc) using DP-seq
(Figure 4a, also see Supplementary Methods online). The differ-
ential gene expression analysis revealed a stepwise increase in the
the gradient of Activin A. The most transcriptional diversity was
observed between SB and AA100 samples corresponding to the
two extreme states of pathway activation. By mapping those
transcripts to known Activin A/TGFb pathway components using
Ingenuity pathway analysis (IngenuityH Systems, www.ingenuity.
com), we observed a substantial down-regulation of many of these
genes in response to pathway inhibition via SB (Figure 4b) whereas
Activin A up-regulated these genes.
Graded activation of the Activin A/TGFb signaling pathway
allowed us to identify putative TGFb regulated genes during early
differentiation of mESCs (Figure 4c). Potential TGFb target genes
conditions (in comparison to untreated control) and (ii) the sub-
sequent up/down regulation with higher dosages of Activin A (see
Supplementary Methods online). We identified many of the
expected TGFb target genes, including Cer133, Lefty131, Lefty231,
Foxa234and T30(Figure 4c, bold). Not all expected genes were found
because they either did not meet our stringent classification criteria
(e.g. Nodal30, Nanog35) or they were not expressed in this cellular
context. More importantly, we have identified transcripts that
respond similarly to the graded Activin A/TGFb pathway modu-
lation, which have not been linked previously to the pathway.
Promoter analysis of these transcripts revealed the presence of mul-
tiple FoxH1 binding sites36–38(Asymmetric Elements, ASE) within
10 Kb upstream and downstream of the transcription start site sup-
porting our hypothesis that the Activin A/TGFb signaling pathway
regulates the expression of these transcripts.
Lineage segregation is achieved by regulation of Activin A/TGFb
signaling. Our preliminary experiments with T-GFP mESCs (GFP
driven by Brachyury/T promoter) showed negligible induction of
GFP1cells at day 4 of differentiation upon treatment with SB
(described in Supplementary Methods). The untreated control
condition (SFM) naturally drives mESCs to neuro-ectoderm
lineage with only 5–10% GFP1cells. However, in presence of
Figure 3 | Comparison of DP-seq with Smart-seq on Activin A treated
samples (AA3 and AA100). (a) MA plot of technical replicates obtained
Distribution of unique reads for the low expressed transcripts (RPKM , 3
in Std. RNA-seq library prepared from AA100 sample) obtained in the
three methods. The majority ofthe low expressed transcripts did not show
Smart-seq. (c) A length bias in Smart-seq resulted in higher reads for the
long cDNA species (. 4 Kb) in the DP-seq libraries. (d) Comparable fold
between AA100 and AA3 samples. The amount of mRNA used for
sequencing library generation is shown in parentheses.
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
mesoderm inducing factors such as Activin A (3 ng/mL), . 60% of
the cells were GFP1demonstrating efficient induction of mesoderm
(see Supplementary Fig. S10 online). Neuro-ectoderm associated
transcripts were classified as transcripts significantly up-regulated
in SB and SFM in comparison to AA15 (see Supplementary
Dataset 1 online) and comprised of known neuro-ectoderm
markers (Sox1, Sox2 and Pax6, Figure 5a). We then performed GO
term (biological process annotation) enrichment and KEGG
pathway enrichment to validate our classification (http://david.
abcc.ncifcrf.gov/). Biological processes associated with neuron
differentiation and morphogenesis (see Supplementary Table S2
online) were enriched in the transcript list with the Wnt and
Activin A/TGFb pathway significantly represented (see Supple-
mentary Table S3 online).
To correlate some of the novel neuro-ectodermal transcripts with
embryology, we searched the MGI gene expression database for the
the neuro-ectoderm associated transcripts were not reported in
embryonic day 6.5–7.5 embryos, the stages that correspond to the
studied mESC derived samples. A number of these transcripts, how-
ever, were expressed in neuro-ectoderm derivatives at later stages of
development. To validate the early expression of these transcripts
in the neuro-ectoderm lineage, we used Wnt pathway inhibition
Figure 4 | Graded expression of putative target genes of the Activin A/TGFb signaling pathway in day 4 mESCs. (a) Schematic representation of the
Methods online). (b) Regulation of Activin A pathway components in response to SB-431542 and Activin A. (c) Putative TGFb target genes in
differentiating mESCs at day 4. The heat map corresponds to fold changes observed for transcripts in comparison to untreated control. Putative target
genes were classified as transcripts that followed opposite trends of regulation upon treatment with Activin A and SB. Fifty transcripts were successively
up-regulatedwhile23transcriptsfollowedgradeddown-regulation withincreasingdosagesofActivin A.ThemajorityoftheTGFbtargetgenes(marked
with *) had FoxH1 transcription factor binding sites separated by 30–200 bp (also called ASE) in 10 Kb upstream and downstream of the transcription
start site. Known TGFb target genes are highlighted in bold. Low copy number transcripts (RPKM , 3 in AA3 sample) are displayed in red font.
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
the up-regulation of a number of these neuro-ectoderm associated
transcripts (Figure 5b and Supplementary Fig. S11 online). On the
other hand, transcripts significantly up-regulated in AA15 in com-
parison to SB and SFM were designated as PS associated transcripts
known mesoderm and endoderm markers (T, Mesp1, Foxa2 and
Sox17). GO enrichment analysis (http://david.abcc.ncifcrf.gov/)
revealed biological processes associated with gastrulation, tissue
morphogenesis and tube development (see Supplementary Table
S2 and S3 online).
Graded Activin A/TGFb signaling has been shown to induce dif-
ferent mesoderm and endoderm tissues, correlating with the ante-
roposterior position of progenitors within the PS, with the highest
levels of signaling corresponding to anterior most located progeni-
tors40–42. Transcripts with a maximum fold change between AA100
and AA15 in comparison to other two fold changes (AA3/SFM and
AA15/AA3) should mark anterior PS derivatives, and in our experi-
ments indeed comprised definitive endoderm markers. Conversely,
the majority of the transcripts with maximum fold changes in AA3/
SFM and AA15/AA3 were expected to have a diffused expression
pattern throughout the PS (Figure 5a), which was confirmed by
reported in-situ hybridizations for some of these transcripts43. To
further validate our classification, we studied some of these new
transcripts by posteriorizing Activin A induced-mesoderm with
BMP444–46. Transcripts known to be expressed in the extra-embry-
onic mesoderm and the extreme posterior PS were indeed enriched
and anterior PS transcripts were significantly down-regulated
(Figure 5c). Pan-PS transcripts also exhibited down-regulation by
ing (see Supplementary Fig. S11 online).
has remained a challenge for most of the existing RNA – seq proto-
cols. Random priming strategies amplify from low amounts of RNA,
however, reliable quantitation of low abundant transcripts is not
Figure 5 | Lineage segregation between neuro-ectoderm and PS (mesoderm and definitive endoderm) achieved by modulation of Activin A/TGFb
signaling pathway. (a) Schematic of the mouse embryo at embryonic day 6.5–7.5 with the gradient of Nodal expression (yellow) with the maximum
of the neuro-ectoderm associated genes is depicted (left of the embryo) with their fold changes in different samples in comparison to untreated control.
Theheatmaponthe rightoftheembryodepicts successivefoldchanges ofthePSmarkers withvaryingdosagesoftheActivin A.Thetranscriptswiththe
highest fold change in AA100 incomparison with AA15 are enriched for definitive endoderm andother anterior tissue markers. Other PStranscripts are
expected to have diffused expression pattern all throughout the streak. Genes with known expression in Theiler stages 9–11 of mouse embryo are
of Wnt signaling pathway (IWR-1) induced the neuro-ectoderm lineage. The fold changes are normalized to the AA3 sample. (c) BMP4 enhanced
expression of posterior and extraembryonic mesoderm markers at the expense of anterior markers. Quantitative RT-PCR fold changes for two BMP4
dosages are normalized with respect to Activin A alone induction. Error bars represent the standard deviation in biological replicates (n 5 3). Asterisks
indicate p . 0.05 (Student’s t-test) compared to controls.
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740
strategy14, primer-dimerization and mismatches in the primer-bind-
and low abundant transcripts were significantly under-represented
work addresses these issues by facilitating generation of reliable
sequencing libraries from as low as 50 pg of mRNA. The dynamic
range of our protocol exceeded five orders of magnitude in RNA
concentrations allowing a more reliable detection of the majority
of the low expressed transcripts.
presence of heptamer primer-binding sites on the mouse transcrip-
tome was utilized to amplify more than 80% of known transcripts
(see Supplementary Fig. S2 online) from a small set of 44 heptamer
primers. We optimized PCR conditions for heptamer hybridization
to achieve successful amplification of more than 50,000 different
fragments representing ,18,000 transcripts in the mouse Refseq
mRNA database. A number of considerations were made while
determining the base composition of primers to reduce mis-priming
and primer dimerization. As a result, majority of the reads (55%)
mismatch in primer-binding site. This enabled us to use the entire
read length for alignment to the mouse transcriptome.
Our transcriptome data demonstrated excellent reproducibility
and sensitivity. We were able to reliably estimate up to a 216-fold
change in transcript expression from limiting amounts of mRNA.
Furthermore, fold changes observed in low abundant transcripts
were in perfect agreement with quantitative RT-PCR. Technical rep-
licate data revealed comparable noise in the quantification of tran-
script expression with respect to standard RNA-seq protocols.
Furthermore, the global measurements of transcript expression of
libraries constructed from at least 50 pg of mRNA showed high
correlations with the library made from 10 ng of mRNA.
A standard RNA-seq approach7requires at least 10–100 ng of
mRNA for reliable library generation. To address this issue, a num-
ber of protocols15,18,19were recently developed. DP-seq offers a cost
effective way of generating reliable sequencing library from limiting
amounts of mRNA. The cost of amplification only includes a one-
time purchase of 44 primers (14 bp) that are sufficient to generate
hundreds of sequencing libraries. Our protocol is compatible with
regular first strand cDNA synthesis kits and the polymerases used in
our protocol (Taq and Klenow polymerase) are cheap and readily
available. The processing time required for the generation of a
sequencing library is also short, as DP-seq library preparation does
not require fragmentation of the cDNA library or poly-adenylation
of the 3’ end of the amplicon library. A direct comparison of DP-seq
with Smart-seq revealed comparable transcriptome coverage and
similar technical noise in the quantification of the low expressed
transcripts. Furthermore, DP-seq does not suffer from length bias
of the long cDNA species in the sequencing library. DP-seq primers
amplify select regions of the known transcriptome, as such the
sequencing libraries are devoid of the information regarding RNA
structure (exon usage, TSS, etc.) or uncharacterized transcripts.
Typical RNA-seq protocols do not discriminate against high
abundant transcripts. Consequently, most of the sequencing effort is
spent on a small number of highly abundant transcripts47. We
exploited the PCR biases observed in our protocol to reduce the rep-
resentation of ribosomal transcripts by designing primers that have
less likelihood of hybridizing efficiently to these transcripts. Complete
elimination of the ribosomal transcripts was not achieved because of
the mis-priming of the heptamer primers. It would be desirable to
reduce mis-priming seen in our approach, and further refinements in
the design strategy to address above issues are in progress.
The increased sensitivity of our methodology allowed us to detect
known transcripts that had only been associated with later stages of
germ layer segregation. These findings are of interest since it sup-
precedes overt manifestation of lineage phenotype, at least as tra-
ditionally assayed. This might not be surprising, since lineage com-
mitment probably involves making chromatin of lineage specific
transcripts accessible to transcriptional machinery, and might result
in low-level transcription. Indeed, recent work on the analysis of
activation marks in the promoters of differentiation specific tran-
scripts has demonstrated that promoter activity is detected well
before established landmarks of differentiation are achieved48,49. It
will be very interesting to explore this idea further, at the single cell
determines germ line specification.
than 80% of the mouse transcriptome. Moreover, while hexamer primers have a low
range of annealing temperatures, heptamer primers hybridize with greater efficiency
to allow Klenow polymerase to extend these primers and perform efficient
We first implemented a suffix array data structure to identify 32-mer unique
regions in the mouse transcriptome. All suffixes in the suffix array were divided into
disjoint segments using 32-mer sequences. For each segment, we then identified all
related segments that possess up to 2 mismatches with the 32-mer sequence. If the
segment and all of its related segments contained suffixes mapping to only one
transcript, then the segment was designated as unique. Next, we predicted the local
secondary structures of the known transcripts as stable secondary structures were
expected to shield heptamer primers from hybridizing to their primer-binding site.
For each transcript in the Mouse NCBI RefSeq mRNA database, we ran a window of
47 bp along the transcript length and determined its propensity to form stable sec-
ondary structure using UNAfold software50. Gibbs free energy (DG) was estimated at
37uC for standard PCR buffer conditions (2 mM MgCl2and 50 mM NaCl). Regions
with a DG $ 24 kcal/mol were considered to be available for heptamer primer
hybridization (open configuration).
We combined the two datasets and identified all heptamer primer-binding sites,
(i) flanking unique regions on mouse transcriptome and (ii) residing in open con-
figuration. We then implemented an iterative randomized algorithm (see
Supplementary Fig.S1 online) toidentify adefinedset of heptamer primers forming
valid amplicons for .80% of the mouse transcriptome. We defined a valid amplicon
It has a length between 50 and 300 bp.
Both, forward and reverse primer-binding sites are in open configuration.
At least one of the primer-binding sites must have a DG $22 Kcal/mol.
A 32 bp unique region should follow one of the primer-binding sites.
The GC content of the amplicon should not exceed 58%.
The amplicon must be within 5 Kb of the 3’ end.
reduce primer-dimerization (see Supplementary Table S4). This configuration
covered ,80% of transcripts with 57% of transcripts covered uniquely. More than
170000 valid amplicons were predicted from 201242 primer-binding sites.
The three primer sets used for suppressing the representation of the ribosomal
transcripts are detailed in Supplementary Table S5 online.
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This work was supported by National Institutes of Health (NIH), National Institute of
Diabetes and Digestive and Kidney Diseases Grant P01-DK074868 (S.S.), National Heart,
Lung and Blood Institute Grant 5 R33 HL087375-02 (S.S. and M.M.), and NIH/National
Institute of General Medical Sciences Grant GM078005-05 (S.S.). V.B. was recipient of a
TG2-01154, Interdisciplinary Stem Cell Training Program at UCSD). E.W. was a fellow
supported by a CIRM postdoctoral training grant T2-00004 at SBMRI and by an AHA
data analysis and useful discussions, Dr. Suresh Subramani, Dr. Brian Saunders, Harish
Nagarajan, Shakti Gupta and Dr. Gaurav Agarwal for many helpful discussions and Dr.
Juan Carlos Izpisu ´a Belmonte for providing T-GFP mESCs.
S.S. and V.B. conceived the designed primer strategy, V.B. and P.K. developed the
methodology for primers, V.B. developed and implemented the sample preparation
protocol, E.W., V.B., M.M., S.S. conceived and designed the embryonic stem cell
experimentsandanalyses.V.B. and E.W. carriedoutthestem cellpreparationand cultured
embryonic stem cells, V.B. wrote the first draft of the manuscript and S.S. and M.M.
supervised the work and revised the manuscript.
Supplementary information accompanies this paper at http://www.nature.com/
Competing financial interests: The authors declare no competing financial interests.
License: This work is licensed under a Creative Commons
Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this
license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
How to cite this article: Bhargava, V., Ko, P., Willems, E., Mercola, M. & Subramaniam, S.
Quantitative Transcriptomics using Designed Primer-based Amplification. Sci. Rep. 3,
1740; DOI:10.1038/srep01740 (2013).
SCIENTIFIC REPORTS | 3 : 1740 | DOI: 10.1038/srep01740