Enumeration of bacteriophages by double agar overlay plaque assay.

Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2009; 501:69-76. DOI: 10.1007/978-1-60327-164-6_7
Source: PubMed

ABSTRACT The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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