Article

Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees.

Brookhaven National Laboratory, Biology Department, Upton, NY 11973-5000, USA.
Applied and Environmental Microbiology (Impact Factor: 3.95). 01/2009; 75(3):748-57. DOI: 10.1128/AEM.02239-08
Source: PubMed

ABSTRACT The association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. Our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (Populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. Members of the Gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. As representatives for the dominant genera of endophytic gammaproteobacteria, we selected Enterobacter sp. strain 638, Stenotrophomonas maltophilia R551-3, Pseudomonas putida W619, and Serratia proteamaculans 568 for genome sequencing and analysis of their plant growth-promoting effects, including root development. Derivatives of these endophytes, labeled with gfp, were also used to study the colonization of their poplar hosts. In greenhouse studies, poplar cuttings (Populus deltoides x Populus nigra DN-34) inoculated with Enterobacter sp. strain 638 repeatedly showed the highest increase in biomass production compared to cuttings of noninoculated control plants. Sequence data combined with the analysis of their metabolic properties resulted in the identification of many putative mechanisms, including carbon source utilization, that help these endophytes to thrive within a plant environment and to potentially affect the growth and development of their plant hosts. Understanding the interactions between endophytic bacteria and their host plants should ultimately result in the design of strategies for improved poplar biomass production on marginal soils as a feedstock for biofuels.

0 Followers
 · 
171 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we isolated putative plant-growth-promoting endophytic bacteria from selenium-supplemented wheat grown under field conditions. These bacterial strains belonged to Bacillus, Paenibacillus, Klebsiella, and Acinetobacter genera and showed genetic similarly with rhizospheric bacteria isolated in the same Andisol soil and with other endophytic strains previously reported. Strains isolated from selenium-supplemented wheat were highly tolerant to elevated selenium concentration (ranged from 60 to 180 mM), and showed potential plant-growth-promoting capabilities (auxin and siderophore production, phytate mineralization, and tricalcium phosphate solubilization). In addition, some strains like Acinetobacter sp. (strain E6.2), Bacillus sp. (strain E8.1), Bacillus sp., and Klebsiella sp. (strains E5 and E1) inhibited the growth of Gaeumannomyces graminis mycelia in vitro at 100, 50, and 30 %, respectively. These endophytic microorganisms would be useful for dual purposes: selenium biofortification of wheat plants and control of G. graminis, the principal soil-borne pathogen in volcanic soils from southern Chile.
    Biology and Fertility of Soils 08/2014; 50(6):983-990. DOI:10.1007/s00374-014-0920-0 · 3.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Plant-bacterial asscociations can improve the degradation of organic pollutants in soil. However, little is known about the diversity and distribution of endophytic bacteria associated with aquatic plants and their potential to enhance phytoremediation of aquatic environments. In the present study, endophytic bacteria were isolated from 4 species of aquatic plants: Phragmites communis, Potamogeton crispus, Nymphaea tetragona and Najas marina. The isolated bacteria were classified into 12 genera in the Gammaproteobacteria, Bacilli, Alphaproteobacteria, Flavobacteria and Actinobacteria. In addition, different strains and/or different concentrations of the bacteria were isolated from different parts of the 4 plants, suggesting the different parts of the 4 plants harbored different endophytic bacteria. Some of the isolates de graded naphthalene and pesticides and some showed potential to dissolve insoluble phosphate. This is one of the first studies to isolate and compare culturable endophytic bacteria among different aquatic plants. This research indicates that culturable endophytes in aquatic plants are very diverse, but are dominated by Gammaproteobacteria, and have the potential to enhance in situ phytoremediation.
    Aquatic Biology 05/2012; 15(2):99-110. DOI:10.3354/ab00422 · 1.12 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Here, we present the draft genome of Enterobacter sp. strain UCD-UG_FMILLET. This strain is an endophyte isolated from the roots of finger millet, an Afro-Indian cereal crop. The genome contains 4,801,411 bp in 53 scaffolds. Copyright © 2015 Ettinger et al.
    Genome Announcements 01/2015; 3(1). DOI:10.1128/genomeA.01461-14

Full-text (2 Sources)

Download
69 Downloads
Available from
May 26, 2014