Dioxin and dioxin-like PCB profiles in the serum of industrial and municipal waste incinerator workers in Korea.
ABSTRACT To assess occupational exposure, we determined the concentrations of PCDD/Fs and PCBs in human serum samples from 26 incinerator workers (10 industrial waste and 16 municipal solid waste incinerator workers), 38 residents near the facilities and 7 inhabitants (as control subjects) living over 10 km away from any incinerator facilities in Korea. The mean TEQ(WHO) levels of PCDD/Fs in the industrial and MSWI workers were 41.57 and 9.86 pg TEQ(WHO) g(-1) lipid, respectively. For the residents, the mean TEQ(WHO) was 13.47 pg TEQ(WHO) g(-1) lipid (residents near IWI, MSWI, and control subjects: 17.64, 13.31, and 6.91 pg TEQ(WHO) g(-1) lipid). Higher levels of certain PCDD/F congeners, mainly PCDFs, were detected in the serum of industrial incinerator workers in comparison to the levels measured in the residents. Significant differences were observed for PCDFs, the major compounds were OCDF, 1,2,3,4,6,7,8-HpCDF, and the minor components 1,2,3,4,7,8-HxCDF, 1,2,3,6,7,8-HxCDF, and 2,3,4,6,7,8-HxCDF (p<0.01). The PCDD/F congener patterns and concentrations measured in the 71 serum samples examined suggest that the industrial incinerator workers were exposed to PCDD/Fs in the workplace, possibly through inhalation and/or skin contact. In contrast, the levels and congener patterns of PCBs measured were similar in all subjects, indicating that workers from the incinerator facilities examined were not subjected to additional exposure to these compounds.
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ABSTRACT: Serum concentrations of environmental pollutants have been positively correlated with diabetes and metabolic syndrome in epidemiologic studies. In turn, abnormal mitochondrial function has been associated with the diseases. The relationships between these variables, however, have not been studied. We developed novel cell-based aryl hydrocarbon receptor (AhR) agonist bioassay system without solvent extraction process and analyzed whether low-dose circulating AhR ligands in human serum are associated with parameters of metabolic syndrome and mitochondrial function. Serum AhR ligand activities were measured as serum 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalent (sTCDDeq) in pM using 10 μL human sera from 97 Korean participants (47 with glucose intolerance and 50 matched controls, average age of 46.6 ± 9.9 years, 53 male and 45 female). sTCDDeq were higher in participants with glucose intolerance than normal controls and were positively associated (P < 0.01) with obesity, blood pressure, serum triglyceride, and fasting glucose, but not with HDL-cholesterol. Body mass index was in a positive linear relationship with serum AhR ligands in healthy participants. When myoblast cells were incubated with human sera, ATP generating power of mitochondria became impaired in an AhR ligand concentration-dependent manner. Our results support that circulating AhR ligands may directly reduce mitochondrial function in tissues, leading to weight gain, glucose intolerance, and metabolic syndrome. Our rapid cell-based assay using minute volume of human serum may provide one of the best monitoring systems for circulating AhR ligands, good clinical biomarkers for the progress of disease and therapeutic efficacy. © 2013 BioFactors, 2013.BioFactors 01/2013; · 3.09 Impact Factor
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ABSTRACT: The construction in Constantí (Tarragona County, Catalonia) of the first, and up till now the only hazardous waste incinerator (HWI) in Spain, finished in 1999. In this study, we measured the concentrations of a number of organic substances determined in blood and urine of the HWI workers. Samples of 18 men and 9 women, classified according to their respective workplaces, were collected in 2011, after approximately 12 years of regular operations in the facility. The current results were compared with those of the baseline survey, as well as with the most recent surveys performed in 2008, 2009 and 2010. Plasma analyses were carried out for hexachlorobenzene (HCB), polychlorinated biphenyls (PCBs 28, 52, 101, 138, 153, and 180) and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), while the levels of 2,4- and 2,5-dichlorophenol (DCP), 2,4,5- and 2,4,6-trichlorophenol (TCP), pentachlorophenol (PCP) and 1-hydroxypyrene (1-HP) were measured in urine samples. In plasma, the mean concentrations were the following: 10.8μg/kg lipid for HCB; 0.8μg/kg lipid for PCB28; 0.3μg/kg lipid for PCB52; 0.5μg/kg lipid for PCB101; 42.2μg/kg lipid for PCB138; 18.5μg/kg lipid for PCB153, and 51.2μg/kg lipid for PCB180. For PCDD/Fs, the mean concentration was 4.6ng I-TEQ/kg lipid (4.7ng WHO-TEQ/kg lipid). These levels, as well as those found in urine samples, are in agreement with the data of previous surveys performed in the same area. The current results in HWI workers do not show any evident sign of occupational exposure to PCDD/Fs and other organic substances. However, these results must be considered only as an indication of potential exposure, as the study presents notable limitations, such as the reduced number of participants and the lack of data relative to the air concentrations of chemicals. Consequently, general conclusions cannot be derived and the results should not be used as a basis for the implementation of industrial hygiene measures in other HWIs.International journal of hygiene and environmental health 02/2013; · 2.64 Impact Factor
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ABSTRACT: Polychlorinated dibenzo-p-dioxin/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) were measured in 954 and 539 human serum samples obtained, respectively, from incinerator workers and residents living near and far from municipal solid waste incinerators (MSWIs) during a 10-year period from 2001 to 2011 in Seoul, Korea. The mean toxic equivalent (TEQ) concentrations of PCDD/Fs and DL-PCBs were 9.29 and 5.39pgTEQ/g lipid, respectively. All populations exhibited similar concentrations and profiles, and 2,3,4,7,8-PeCDF, 1,2,3,7,8-PeCDD, and PCB126 were identified as major contributors to TEQs. However, the levels of PCDD/Fs and DL-PCBs were found to have positive associations with age when considering stratified age groups and showed a slight trend toward reduction. Differences in concentrations of these compounds among age groups slightly but continuously decreased from 2001 to 2011. The measured PCDD/F levels were compared to the predicted concentrations by using a single-compartment pharmacokinetic model on the basis of the daily intake of PCDD/Fs. The predicted concentration was 5.24pgTEQ/g lipid, which was approximately half of the measured concentration, reflecting the present exposures. These results suggest that current data on the levels of PCDD/Fs in foodstuffs might be useful to predict the future levels of dioxins in humans, which are expected to decline with time.Science of The Total Environment 08/2013; · 3.26 Impact Factor