Identification of microRNA targets in tomato fruit development using high-throughput sequencing and degradome analysis.
ABSTRACT MicroRNAs (miRNAs) play important roles in plant development through regulation of gene expression by mRNA degradation or translational inhibition. Despite the fact that tomato (Solanum lycopersicum) is the model system for studying fleshy fruit development and ripening, only a few experimentally proven miRNA targets are known, and the role of miRNA action in these processes remains largely unknown. Here, by using parallel analysis of RNA ends (PARE) for global identification of miRNA targets and comparing four different stages of tomato fruit development, a total of 119 target genes of miRNAs were identified. Of these, 106 appeared to be new targets. A large part of the identified targets (56) coded for transcription factors. Auxin response factors, as well as two known ripening regulators, COLORLESS NON-RIPENING (CNR) and APETALA2a (SlAP2a), with developmentally regulated degradation patterns were identified. The levels of the intact messenger of both CNR and AP2a are actively modulated during ripening, by miR156/157 and miR172, respectively. Additionally, two TAS3-mRNA loci were identified as targets of miR390. Other targets such as ARGONAUTE 1 (AGO1), shown to be involved in miRNA biogenesis in other plant species, were identified, which suggests a feedback loop regulation of this process. In this study, it is shown that miRNA-guided cleavage of mRNAs is likely to play an important role in tomato fruit development and ripening.
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ABSTRACT: TCP proteins are plant-specific transcription factors, which are known to have a wide range of functions in different plant species such as in leaf development, flower symmetry, shoot branching, and senescence. Only a small number of TCP genes has been characterised from tomato (Solanum lycopersicum). Here we report several functional features of the members of the entire family present in the tomato genome.BMC Plant Biology 06/2014; 14(1):157. · 4.35 Impact Factor
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ABSTRACT: MicroRNAs are ~22 nt non-coding endogenous RNAs which play important regulation roles in various species. By using homology-based computational research, 14 novel and conserved tomato miRNAs belonging to ten families were identified from EST, GSS, and nucleotide sequences. Real-time PCR analysis of these miRNAs demonstrated their expression in tomato afterwards. Meanwhile, a total of 36 potential targets were predicted for the ten miRNAs using psRNATarget. The target genes were mainly involved in metabolism, transmembrane transport, stress response, and transcription regulation. According to our experiment, miR398 was down-regulated on different levels under biotic and abiotic stresses, suggesting that miR398 might be involved in tomato stress regulatory network. Our results supplement the findings of tomato miRNAs and also suggest crucial regulatory functions of miRNAs in stress responses.Molecular Biology Reports 05/2014; · 2.51 Impact Factor
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ABSTRACT: In plants, microRNAs (miRNAs) regulate gene expression mainly at the post-transcriptional level. Previous studies have demonstrated that miRNA-mediated gene silencing pathways play vital roles in plant development. Here, we used a high-throughput sequencing approach to characterize the miRNAs and their targeted transcripts in the leaf, flower and fruit of sweet orange.BMC genomics. 08/2014; 15(1):695.