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Determinación de cobre, magnesio y zinc en leucocitos mononucleares mediante espectrometría de absorción atómica con llama

Investigación clínica (Impact Factor: 0.39). 03/2013; 53(4):342 - 352.

ABSTRACT Palabras clave: leucocitos mononucleares, elementos traza, microinyector, espec-trometría de absorción atómica. Resumen. En este trabajo se evaluó un nuevo microinyector de inyección en flujo para la determinación de Cu, Mg y Zn en células mononucleares de la sangre. Este dispositivo permitió analizar muestras en el orden de microlitros mediante espectrometría de absorción atómica con llama, es fácil de cons-truir y adaptar al inyector convencional del espectrofotómetro de absorción atómica. En la determinación de Cu, Mg y Zn se obtuvieron límites de detec-ción de 106, 65 y 37 µg L –1 , respectivamente. En las pruebas de recuperación de estos elementos en leucocitos mononucleares se encontraron porcentajes de recuperación entre 98 y 110%. Determination of copper, magnesium and zinc in mononuclear leukocytes by flame atomic absorption spectrometry. Invest Clin 2012; 53(4): 342 -352 Abstract. In this paper we evaluated a new micro-flow injector for the determination of the concentrations of Cu, Mg and Zn in mononuclear blood cells. This device analyzed sample volumes in the order of microliters by flame atomic absorption spectrometry; it is inexpensive, and easy to build and to adapt to the conventional injector of the atomic absorption spectrophotometer. Detection limits of 106, 65 and 37 µg L –1 for Cu, Mg and Invest Clin 53(4): 342 -352, 2012

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