Molecular phylogenetic analysis of the coccidian cephalopod parasites Aggregata octopiana and Aggregata eberthi (Apicomplexa: Aggregatidae) from the NE Atlantic coast using 18S rRNA sequences

Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello 6, 36208 Vigo, Spain.
European Journal of Protistology (Impact Factor: 2.34). 03/2013; DOI: 10.1016/j.ejop.2012.11.005

ABSTRACT The coccidia genus Aggregata is responsible for intestinal coccidiosis in wild and cultivated cephalopods. Two coccidia species, Aggregata octopiana, (infecting the common octopus Octopus vulgaris), and A. eberthi, (infecting the cuttlefish Sepia officinalis), are identified in European waters. Extensive investigation of their morphology resulted in a redescription of A. octopiana in octopuses from the NE Atlantic Coast (NW Spain) thus clarifying confusing descriptions recorded in the past. The present study sequenced the 18S rRNA gene in A. octopiana and A. eberthi from the NE Atlantic coast in order to assess their taxonomic and phylogenetic status. Phylogenetic analyses revealed conspecific genetic differences (2.5%) in 18S rRNA sequences between A. eberthi from the Ria of Vigo (NW Spain) and the Adriatic Sea. Larger congeneric differences (15.9%) were observed between A. octopiana samples from the same two areas, which suggest the existence of two species. Based on previous morphological evidence, host specificity data, and new molecular phylogenetic analyses, we suggest that A. octopiana from the Ria of Vigo is the valid type species. Published by Elsevier GmbH.

1 Bookmark
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Apicomplexan parasites represent one of the most important groups of parasitic unicellular eukaryotes comprising such important human parasites such as Plasmodium spp. and Toxoplasma gondii. Apicomplexan radiation as well as their adaptation to the parasitic style of life took place before the era of vertebrates. Thus, invertebrates were the first hosts of apicomplexan parasites that switched to vertebrates later in evolution. Despite this fact, apicomplexan parasites of invertebrates, with the exception of gregarines, have so far been ignored in phylogenetic studies. To address this issue, we sequenced the nuclear SSU rRNA genes from the homoxenous apicomplexan parasites of insects Adelina grylli and Adelina dimidiata, and the heteroxenous Aggregata octopiana and Aggregata eberthii that are transmitted between cephalopods and crustaceans, and used them for phylogenetic reconstructions. The position of the adelinids as a sister group to Hepatozoon spp. within the suborder Adeleorina was stable regardless of the phylogenetic method used. In contrast, both members of the genus Aggregata possess highly divergent SSU rRNA genes with an unusual nucleotide composition. Because of this, they form the longest branches in the tree and their position is variable. However, the genus Aggregata branches together with adelinids and hepatozoons in most of the analyses, although their position within the scope of this cluster is unstable.
    Protist 07/2006; 157(2):173-83. · 3.56 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Gamogony and sporogony of Aggregata octopiana were commonly observed during histological examination of the digestive tract of wild Octopus vulgaris from Ria de Vigo (NW Spain). A. octopiana infected noncuticularized caecum and intestine, and cuticularized oesophagus and crop. Infection was also observed in the gills and in covering mesenterium, mainly of the digestive gland and gonad. Histological and ultrastructural lesions associated with A. octopiana included host cell hypertrophy with nuclear displacement, inflammation, phagocytosis, ulceration and destruction of organ architecture. The possible existence of a malabsorption syndrome in the host is deduced.
    Diseases of Aquatic Organisms 07/2002; 50(1):45-9. · 1.59 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This paper represents an updated review of the published and unpublished records of parasites collected from cephalopod molluscs (teuthoid squids, cuttlefish and octopods) in waters off Spain, with special mention for those inhabiting coastal and shelf waters off Galicia (NW Spain). The examination of about 1600 cephalopods in the southeastern North Atlantic Ocean revealed a new parasite species Stellicola hochbergi, 37 new host records for Atlanto-Iberian waters, and 18 new host records for the world Ocean (three for coccidian Aggregatidae; three and one for cestodes Phyllobothriidae and Tentaculariidae; five and one for nematodes Anisakidae and Cystidicolidae; one and four for copepods Lichomolgidae and Pennellidae). Results suggests the important role of cephalopods as intermediate or final hosts in the life cycles of ten systematic groups of parasites.
    Aquaculture 06/1996; 142:1-10. · 1.83 Impact Factor


Available from
May 21, 2014