Article

Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA. Electronic address: .
Developmental Biology (Impact Factor: 3.64). 03/2013; 377(2). DOI: 10.1016/j.ydbio.2013.02.019
Source: PubMed

ABSTRACT Cell polarity plays an important role in tissue morphogenesis; however, the mechanisms of polarity and their role in mammalian development are still poorly understood. We show here that membrane-associated guanylate kinase protein Dlg5 is required for proper branching morphogenesis and progenitor differentiation in mammalian lung. We found that during lung development Dlg5 functions as an apical-basal polarity protein, which is necessary for the apical maintenance of atypical protein kinase C (aPKC). These results identify Dlg5 as a regulator of apical polarity complexes and uncover the critical function of Dlg5 in branching morphogenesis and differentiation of lung progenitor cells.

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    • "ZO1 interacts with ZO2 and ZO3 via their PDZ2 domains to form a tight junctional complex. DLG5 participates in regulation of cell polarity by promoting the apical localization of aPKC 31. It has been found that aPKC and ZO1 are mislocalized, while PAR3 and remains apically localized in DLG5 knockout mice 22, 31. "
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    • "Values expressed as mean RPKM. a RPKM values were calculated following alignment of fastq files from the Illumina BodyMap 2.0 project. polarity complexes [20] and epithelial-to-mesenchymal transition (EMT) [21]. Cytotrophoblast fusion requires loss of cellecell junctions [22], not dissimilar to EMT, suggesting that DLG5 may be important for the regulation of syncytialization. "
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