The effect of calcium silicate on in vitro physiochemical properties and in vivo osteogenesis, degradability and bioactivity of porous β-tricalcium phosphate bioceramics.
ABSTRACT Porous β-tricalcium phosphate(TCP)/calcium silicate(CS) composite bioceramics with different weight proportions were prepared to investigate the in vitro effects of CS on the physiochemical properties of TCP and the in vivo effects of CS on the degradability, osteogenesis and bioactivity of TCP. The physiochemical results showed that the addition of CS to porous TCP resulted in a looser and rougher surface and a lower solid density, compressive strength and Young's modulus and a lower pH value as compared to pure CS without any chemical interaction between the TCP and the CS. The in vivo study showed that the material degradation of porous TCP/CS composite bioceramics was slower than that of pure CS, although the osteogenesis, degradability and bioactivity were significantly increased in the long term. Thereafter, the introduction of CS into porous TCP bioceramics is an effective way to prepare bioactive bone grafting scaffolds for clinical use and to control properties such as in vivo degradability and osteoinduction of TCP.
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ABSTRACT: β-Tricalcium phosphate (β-TCP) is an osteoconductive material. For this research we have combined it with a low degradation calcium silicate (CS) to enhance its bioactive and osteostimulative properties. To check its effectiveness, a series of β-TCP/CS composites with different ratios were prepared to make new bioactive and biodegradable biocomposites for bone repair. Regarding the formation of bone-like apatite, the diametral tensile strength as well as the ion release and weight loss of composites were compared both before and after immersions in simulated body fluid (SBF). In addition, we also examined the behavior of human dental pulp cells (hDPCs) cultured on β-TCP/CS composites. The results show that the apatite deposition ability of the β-TCP/CS composites improves as the CS content is increased. For composites with more than a 60% CS content, the samples become completely covered by a dense bone-like apatite layer. At the end of the immersion period, weight losses of 24%, 32%, 34%, 38%, 41%, and 45% were observed for the composites containing 0%, 20%, 40%, 80%, 80% and 100% β-TCP cements, respectively. In addition, the antibacterial activity of CS/β-TCP composite improves as the CS-content is increased. In vitro cell experiments show that the CS-rich composites promote human dental pulp cell (hDPC) proliferation and differentiation. However, when the CS quantity in the composite is less than 60%, the quantity of cells and osteogenesis protein of hDPCs is stimulated by Si released from the β-TCP/CS composites. The degradation of β-TCP and the osteogenesis of CS give strong reason to believe that these calcium-based composite cements will prove to be effective bone repair materials.Materials Science and Engineering C 10/2014; 43:126–134. DOI:10.1016/j.msec.2014.06.030 · 2.74 Impact Factor
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ABSTRACT: Fabrication of mechanically competent bioactive scaffolds is a great challenge in bone tissue engineering. In this paper, β-tricalcium phosphate (β-TCP) scaffolds were successfully fabricated by selective laser sintering combined with furnace sintering. Bioglass 45S5 was introduced in the process as liquid phase in order to improve the mechanical and biological properties. The results showed that sintering of β-TCP with the bioglass revealed some features of liquid phase sintering. The optimum amount of 45S5 was 5 wt %. At this point, the scaffolds were densified without defects. The fracture toughness, compressive strength and stiffness were 1.67 MPam1/2, 21.32 MPa and 264.32 MPa, respectively. Bone like apatite layer was formed and the stimulation for apatite formation was increased with increase in 45S5 content after soaking in simulated body fluid, which indicated that 45S5 could improve the bioactivity. Furthermore, MG-63 cells adhered and spread well, and proliferated with increase in the culture time.International Journal of Molecular Sciences 08/2014; 15(8):14574-90. DOI:10.3390/ijms150814574 · 2.34 Impact Factor
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ABSTRACT: The aim of this study was to assess the bone regeneration of critical size defects in rabbit calvarias filled with β-TCP doped with silicon.Clinical Oral Implants Research 05/2014; DOI:10.1111/clr.12413 · 3.43 Impact Factor