Combined Effects of Genistein, Quercetin, and Resveratrol in Human and 3T3-L1 Adipocytes

Department of Animal & Dairy Science, University of Georgia, Athens, Georgia 30602-2771, USA.
Journal of medicinal food (Impact Factor: 1.63). 01/2009; 11(4):773-83. DOI: 10.1089/jmf.2008.0077
Source: PubMed


The natural compounds genistein (G), quercetin (Q), and resveratrol (R) have been reported to each exhibit anti-adipogenic activities in adipocytes and antiproliferative and pro-apoptotic activities in several cell types. We studied the combined effects of G, Q, and R on adipogenesis and apoptosis in primary human adipocytes (HAs) and 3T3-L1 murine adipocyte (MAs). Combined treatment with 6.25 microM G, 12.5 microM Q, and 12.5 microM R during the 14-day differentiation period caused an enhanced inhibition of lipid accumulation in maturing HAs that was greater than the responses to individual compounds and to the calculated additive response. Glycerol 3-phosphate dehydrogenase activity, a marker of late adipocyte differentiation, was decreased markedly in HAs treated with the combination of G+Q+R. In addition, combined treatment with 50 microM G, 100 microM Q, and 100 microM R for 3 days decreased cell viability and induced apoptosis in early- and mid- phase maturing and lipid-filled mature HAs. In contrast, no compound alone induced apoptosis. Oil Red O stain and Hoechst 33342 stain were performed to confirm the effects on lipid accumulation and apoptosis, respectively. We also determined whether MAs responded to the combination treatment similarly to HAs. As in HAs, G+Q+R treatment decreased lipid accumulation in maturing MAs and increased apoptosis in pre- and lipid-filled mature MAs more than the responses to G, Q, and R when used separately. These results show that lower concentrations of combined treatments with several natural compounds may be useful for treatments for obesity through the suppression of adipogenesis and enhanced adipocyte apoptosis.

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Available from: Mary Anne Della-Fera, Jan 09, 2014
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    • "In this study, the PSE supplementation (containing 0.05% resveratrol) showed significantly decreased visceral adipose fat weights, even much higher effects than pure 0.05 % resveratrol supplementation reported by previous studies [9,10,11,12]. Two previous in vitro studies reported that combination treatment with resveratrol and genistein or other natural polypherols suppressed lipid accumulation significantly more than the responses to resveratrol alone [25,26]. Those studies suggested that resveratrols's anti-adipogenic activity was substantially enhanced by the other natural isoflavornes or pholypherols compound in foods. "
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    • "Natural products have the potential to inhibit adipogenesis and induce the apoptosis of mature adipocytes [5,6]. Herbal extracts and phytochemicals have shown anti-obesity effects, especially the down-regulation of the adipogenic transcriptional factors peroxisome proliferator-activated receptor-γ (PPAR-γ) and CCAAT/enhancer binding protein-α (C/EBP-α), the inhibition of fatty acid accumulation, and the stimulation of glycerol-3-phosphate dehydrogenase (GPDH) activity in 3 T3-L1 preadipocytes [7-10]. Berberine, a natural plant product, displays beneficial effects in the treatment of diabetes and obesity at least in part via the stimulation of AMP-activated protein kinase (AMPK) activity [11]. "
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    BMC Complementary and Alternative Medicine 10/2013; 13(1):282. DOI:10.1186/1472-6882-13-282 · 2.02 Impact Factor
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    • "We also previously reported that after six days of 10, 20, and 40 µmol/L resveratrol treatment, lipid accumulation and GPDH activity in adipocyte was significantly inhibited as a dose-dependent response (P < 0.05) [34]. Park et al. [35]'s findings were also consistent with our study in showing that resveratrol treatment of 3T3-L1 adipocytes had a significant apoptosis and inhibition of GPDH activity. "
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