Optofluidic cell manipulation for a biological microbeam
Department of Mechanical Engineering, Columbia University, New York, New York 10027, USA.The Review of scientific instruments (Impact Factor: 1.61). 02/2013; 84(1):014301. DOI: 10.1063/1.4774043
This paper describes the fabrication and integration of light-induced dielectrophoresis for cellular manipulation in biological microbeams. An optoelectronic tweezers (OET) cellular manipulation platform was designed, fabricated, and tested at Columbia University's Radiological Research Accelerator Facility (RARAF). The platform involves a light induced dielectrophoretic surface and a microfluidic chamber with channels for easy input and output of cells. The electrical conductivity of the particle-laden medium was optimized to maximize the dielectrophoretic force. To experimentally validate the operation of the OET device, we demonstrate UV-microspot irradiation of cells containing green fluorescent protein (GFP) tagged DNA single-strand break repair protein, targeted in suspension. We demonstrate the optofluidic control of single cells and groups of cells before, during, and after irradiation. The integration of optofluidic cellular manipulation into a biological microbeam enhances the facility's ability to handle non-adherent cells such as lymphocytes. To the best of our knowledge, this is the first time that OET cell handling is successfully implemented in a biological microbeam.
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ABSTRACT: An acoustic-based method for manipulating particles using phase-shifting is demonstrated. The location of the pressure node was changed simply by adjusting the phase difference (phase-shift) applied to the two interdigital transducers in the design. As a result, polystyrene particles of 5 μm diameter trapped in the pressure node were manipulated laterally across the microchannel fabricated. The lateral particle displacement from −72.5 μm to 73.1 μm along the x-direction was accomplished by varying the phase-shift with a range of −180° to 180°. In this paper, the particle displacement as a function of the phase-shift of SAW was obtained experimentally and close agreement with the theoretical prediction of the particle position was demonstrated.Sensors and Actuators A Physical 03/2014; 207:39–42. DOI:10.1016/j.sna.2013.12.020 · 1.90 Impact Factor
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ABSTRACT: Noncontact robotic particle grippers with trapping, manipulation, and release functions are highly desired in cell biology and microfluidics. Optoelectric techniques combine optical and electrokinetic effects to create thousands of such individually addressable traps. By projecting reconfigurable light patterns, these techniques can concentrate molecules, as well as manipulate, sort, and electroporate cells in a programmable manner. We describe the underlying physical mechanisms and discuss applications in biology and future prospects of these devices.Trends in Biotechnology 07/2014; 32(8). DOI:10.1016/j.tibtech.2014.06.002 · 11.96 Impact Factor
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