Implications of Adipose-Derived Stromal Cells in a 3D Culture System for Osteogenic Differentiation: An in Vitro and in Vivo Investigation

Department of Orthopaedic Surgery, University of Virginia, Box 800159, Charlottesville, VA 22908, USA. Electronic address: .
The Spine Journal (Impact Factor: 2.43). 01/2013; 13(1):32-43. DOI: 10.1016/j.spinee.2013.01.002
Source: PubMed


Healthy mammalian cells in normal tissues are organized in complex three-dimensional (3D) networks that display nutrient and signaling gradients. Conventional techniques that grow cells in a 2D monolayer fail to reproduce the environment that is observed in vivo. In recent years, 3D culture systems have been used to mimic tumor microenvironments in cancer research and to emulate embryogenesis in stem cell cultures. However, there have been no studies exploring the ability for adipose-derived stromal (ADS) cells in a 3D culture system to undergo osteogenic differentiation.

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    • "ticularly morphological changes , with EADSC in 3D culture adopting a spherical morphology compared to the flat and elongated morphology of EADSC in 2D culture . Studies culturing human adipose derived - stem cells in 3D multicellular aggregates have observed similar results in respect to cellular rounding and enhanced osteogenic differentiation ( Shen et al . 2013 ) , with cell shape previously being demonstrated to influence gene and protein expression in cultured human adipose - derived stem cells ( Amos et al . 2010 ) . These findings will contribute to the design of more physiologically relevant studies regarding the differ - entiation capabilities of EADSC , with the potential for use as an "
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