Properties of Ribbon and Non-Ribbon Release from Rod Photoreceptors Revealed by Visualizing Individual Synaptic Vesicles

Departments of Pharmacology and Experimental Neuroscience and Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198, and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520.
The Journal of Neuroscience : The Official Journal of the Society for Neuroscience (Impact Factor: 6.75). 01/2013; 33(5):2071-86. DOI: 10.1523/JNEUROSCI.3426-12.2013
Source: PubMed

ABSTRACT Vesicle release from rod photoreceptors is regulated by Ca(2+) entry through L-type channels located near synaptic ribbons. We characterized sites and kinetics of vesicle release in salamander rods by using total internal reflection fluorescence microscopy to visualize fusion of individual synaptic vesicles. A small number of vesicles were loaded by brief incubation with FM1-43 or a dextran-conjugated, pH-sensitive form of rhodamine, pHrodo. Labeled organelles matched the diffraction-limited size of fluorescent microspheres and disappeared rapidly during stimulation. Consistent with fusion, depolarization-evoked vesicle disappearance paralleled electrophysiological release kinetics and was blocked by inhibiting Ca(2+) influx. Rods maintained tonic release at resting membrane potentials near those in darkness, causing depletion of membrane-associated vesicles unless Ca(2+) entry was inhibited. This depletion of release sites implies that sustained release may be rate limited by vesicle delivery. During depolarizing stimulation, newly appearing vesicles approached the membrane at ∼800 nm/s, where they paused for ∼60 ms before fusion. With fusion, vesicles advanced ∼18 nm closer to the membrane. Release events were concentrated near ribbons, but lengthy depolarization also triggered release from more distant non-ribbon sites. Consistent with greater contributions from non-ribbon sites during lengthier depolarization, damaging the ribbon by fluorophore-assisted laser inactivation (FALI) of Ribeye caused only weak inhibition of exocytotic capacitance increases evoked by 200-ms depolarizing test steps, whereas FALI more strongly inhibited capacitance increases evoked by 25 ms steps. Amplifying release by use of non-ribbon sites when rods are depolarized in darkness may improve detection of decrements in release when they hyperpolarize to light.

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    • "Slow release is amplified by CICR in both amphibian and mammalian rods (Cadetti et al., 2006; Suryanarayanan and Slaughter, 2006; Babai et al., 2010b) and the present results showed that CICR promotes slow release by triggering vesicle fusion at non-ribbon sites. For example , when rods were stimulated with 500-ms depolarizing steps to activate CICR, ∼50% of release events occurred >1 μm from the ribbon (Chen et al., 2013) and the present results showed that virtually all of the non-ribbon release events evoked by 500-ms steps were triggered by CICR (Figure 5). The evidence for substantial non-ribbon release from rods in retinal slices obtained by FALI experiments suggests that non-ribbon release observed by TIRFM in isolated rods is not an artifact of cell isolation procedures. "
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