Molecular-based surveillance of campylobacteriosis in New Zealand - From source attribution to genomic epidemiology

Epi-interactive, Miramar, Wellington, New Zealand.
Eurosurveillance: bulletin europeen sur les maladies transmissibles = European communicable disease bulletin (Impact Factor: 5.72). 01/2013; 18(3).
Source: PubMed


Molecular-based surveillance of campylobacteriosis in New Zealand contributed to the implementation of interventions that led to a 50% reduction in notified and hospitalised cases of the country's most important zoonosis. From a pre-intervention high of 384 per 100,000 population in 2006, incidence dropped by 50% in 2008; a reduction that has been sustained since. This article illustrates many aspects of the successful use of molecular-based surveillance, including the distinction between control-focused and strategyfocused surveillance and advances in source attribution. We discuss how microbial genetic data can enhance the understanding of epidemiological explanatory and response variables and thereby enrich the epidemiological analysis. Sequence data can be fitted to evolutionary and epidemiological models to gain new insights into pathogen evolution, the nature of associations between strains of pathogens and host species, and aspects of between-host transmission. With the advent of newer sequencing technologies and the availability of rapid, high-coverage genome sequence data, such techniques may be extended and refined within the emerging discipline of genomic epidemiology. The aim of this article is to summarise the experience gained in New Zealand with molecular- based surveillance of campylobacteriosis and to discuss how this experience could be used to further advance the use of molecular tools in surveillance.

Download full-text


Available from: Nigel P French, Jan 05, 2014
1 Follower
26 Reads
  • Source
    • "Molecular or phenotypic markers selected for epidemiological investigation should provide enough differentiation to separate, for instance, outbreak and non-outbreak isolates. Source tracking initiatives have also found it useful to group larger populations into clones or lineages that are specific for particular animals or environmental niches (Muellner et al., 2013; Sheppard et al., 2010). For many pathogens, molecular typing is performed in the context of outbreak investigation and the rapidity of the response becomes the most important consideration to minimize the ultimate impact of an emerging outbreak (Boxrud et al., 2010; Hedberg et al., 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Campylobacter remains one of the most common bacterial causes of gastroenteritis worldwide. Tracking sources of this organism is challenging due to the large numbers of human cases, and the prevalence of this organism throughout the environment due to growth in a wide range of animal species. Many molecular subtyping methods have been developed to characterize Campylobacter species, but only a few are commonly used in molecular epidemiology studies. This review examines the applicability of these methods, as well as the role that emerging whole genome sequencing technologies will play in tracking sources of Campylobacter spp. infection.
    Journal of microbiological methods 07/2013; 95(1). DOI:10.1016/j.mimet.2013.07.007 · 2.03 Impact Factor
  • Source
    Article: The role of
    [Show abstract] [Hide abstract]
    ABSTRACT: Although it is known that Escherichia coli O157 is capable of long‐term soil survival, little is known about the mechanisms involved. This study investigated the role of the general stress response system RpoS in E. coli soil survival. The results showed that E. coli O157 isolates capable of long‐term survival (longer than 200 days) in manure‐amended soil were characterized by the absence of mutations in their rpoS gene. In contrast, the strains not capable of long‐term survival all possessed mutations in their rpoS gene. In addition, the long‐term surviving strains showed significantly higher levels of acid resistance in simulated gastric fluid (pH 2.5). Sequencing of the rpoS gene of bovine, food and clinical isolates revealed a skewed distribution of rpoS wild‐type and mutant strains among the different sources. Bovine and food isolates had low numbers of mutants (RpoS system is an advantage for survival in the manure‐amended soil environment. Further deletion and complementation studies should provide more evidence on the role of RpoS in the long‐term survival of E. coli O157 in diverse environments.
    FEMS Microbiology Letters 01/2013; 338(1). · 2.12 Impact Factor
  • Source
    Eurosurveillance: bulletin europeen sur les maladies transmissibles = European communicable disease bulletin 02/2013; 18(4). · 5.72 Impact Factor
Show more