Article

The effect of bladder outlet obstruction on alpha1- and beta-adrenoceptor expression and function.

Department of Pharmacology and Pharmacotherapy, Academic Medical Center, Amsterdam, The Netherlands.
Neurourology and Urodynamics (impact factor: 2.96). 12/2008; 28(4):349-55. DOI:10.1002/nau.20642 pp.349-55
Source: PubMed

ABSTRACT To explore possible changes in expression and/or function of alpha(1)- and beta-adrenoceptor subtypes as a cause for bladder dysfunction in a rat model of bladder outlet obstruction (BOO).
BOO was induced in rats by partial urethral ligature. Contraction and relaxation experiments were performed with isolated bladder strips from BOO, sham-operated and non-operated (control) rats 7 days after BOO induction. mRNA expression of alpha(1)- and beta-adrenoceptor subtypes was assessed by quantitative real-time PCR.
Receptor-independent contraction or relaxation did not differ between BOO and sham rats. The alpha(1)-agonists methoxamine and A-61,603 caused only weak contraction without major differences between groups. Against KCl-induced tone, the beta-adrenoceptor agonists noradrenaline and isoprenaline caused similar relaxation in BOO and sham rats, whereas relaxation in response to the beta(3)-selective BRL 37,344 was attenuated. Against passive tension, noradrenaline induced relaxation in sham and control rats; in contrast, noradrenaline induced contraction at low concentrations and relaxation at high concentrations in BOO rats. The contraction component was abolished by the alpha(1)-antagonist prazosin. The mRNA expression of alpha(1D)-adrenoceptors was increased in BOO, whereas none of the other receptor mRNAs were up-regulated.
In a rat BOO model, weak contraction responses to alpha(1)-agonists and relaxation responses to beta-agonists are not altered to a major extent. Nevertheless, relaxation responses to the endogenous agonist noradrenaline are turned into alpha(1)-adrenoceptor-mediated contraction responses in BOO, possibly due to an up-regulation of alpha(1D)-adrenoceptors.

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    Article: Modulation of alpha 1 adrenergic receptors on urinary bladder in rat spinal cord injury model.
    Gilho Lee, Heeyoon Park, Hong Suk Park, Jeong Gu Lee
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    ABSTRACT: Whereas many studies have focused on the vesical changes of the α1 adrenergic receptor (AR) subtypes in partial outlet obstruction, few studies have addressed the modulation of the α1 AR subtypes after spinal cord injury (SCI). Therefore, we studied the modulation of the α1 ARs in urinary bladder in a rat SCI model. Four weeks after a SCI, the whole vesical bodies from eight female Sprague-Dawley rats and from eight controls were harvested. The total RNA was extracted from the samples and was used to prepare cDNA. We developed standard plasmid constructs of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and three α1 ARs (α1a, α1b, and α1d) to convert the cycle threshold (Ct) values from real-time polymerase chain reaction (RT-PCR) into subtype mRNA concentrations. The detected Ct values of 16 samples from RT-PCR were interpolated into the standard plasmid curves. All serially diluted standard samples showed very good linearity. The mRNA expression of GAPDH was higher in the SCI group, whereas the mRNA expression of all α1 ARs was lower in the SCI group than in the control animals. The α1a, α1b, and α1d mRNA expression in the controls was 81.7%, 3.3%, and 15.1%, respectively, whereas the α1a, α1b, and α1d mRNA expression in the SCI group was 33.5%, 5.2%, and 60.9%, respectively. SCI moderates the α1 AR mRNA subtypes in the urinary bladder. The relatively increased α1d or decreased α1a AR mRNA expression may be a therapeutic candidate for controlling the symptoms of neurogenic bladder after SCI.
    International neurourology journal 06/2012; 16(2):62-8.
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Keywords

alpha(1)-adrenoceptor-mediated contraction responses
 
alpha(1)-agonists methoxamine
 
alpha(1)-antagonist prazosin
 
beta-adrenoceptor agonists noradrenaline
 
beta-adrenoceptor subtypes
 
bladder dysfunction
 
BOO rats
 
contraction component
 
control rats
 
endogenous agonist noradrenaline
 
noradrenaline induced contraction
 
partial urethral ligature
 
passive tension
 
quantitative real-time PCR
 
rat BOO model
 
rat model
 
receptor mRNAs
 
Receptor-independent contraction
 
sham rats
 
weak contraction responses