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In vitro establishment of seeds of physic nut (Jatropha curcas L.) - Euphorbiaceae.

Caderno de Pesquisa série Biologia 01/2010; 22(3):5-12.

ABSTRACT The aim of this study was to establish in vitro seedlings from seeds of physic nut (Jatropha curcas L.). The disinfection experiment consisted in factorial arrangement (2x2x3), being seed with tegument and without tegument, liquid and semi solid (6 g L-1 agar) media and three concentrations of NaOCl (1%, 2.5% and 5% ). Seeds were imersed in 70% ethanol for one minute and immersed in sodium hypochlorite (1%, 2.5% or 5%) for 10 minutes, after seeds were washed four times with distilled and autoclaved water. Basal germination medium was MS with 30 gL-1 sucrose and pH adjusted to 5.8. The establishment of in vitro plantlets of physic nut can be achieved by disinfection of seeds without tegument and immersion in sodium hypochlorite 2.5%, and germinated on the basic semi-solid medium, reaching 100% of seeds disinfection and 90% of germination.

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