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In vitro establishment of seeds of physic nut (Jatropha curcas L.) - Euphorbiaceae.

Caderno de Pesquisa série Biologia 01/2010; 22(3):5-12.

ABSTRACT The aim of this study was to establish in vitro seedlings from seeds of physic nut (Jatropha curcas L.). The disinfection experiment consisted in factorial arrangement (2x2x3), being seed with tegument and without tegument, liquid and semi solid (6 g L-1 agar) media and three concentrations of NaOCl (1%, 2.5% and 5% ). Seeds were imersed in 70% ethanol for one minute and immersed in sodium hypochlorite (1%, 2.5% or 5%) for 10 minutes, after seeds were washed four times with distilled and autoclaved water. Basal germination medium was MS with 30 gL-1 sucrose and pH adjusted to 5.8. The establishment of in vitro plantlets of physic nut can be achieved by disinfection of seeds without tegument and immersion in sodium hypochlorite 2.5%, and germinated on the basic semi-solid medium, reaching 100% of seeds disinfection and 90% of germination.

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    ABSTRACT: The growth analysis has been used by researchers to explain differences in the growth, which can be caused for genetic factors or by environmental modifications and constitutes efficient tool for the identification of favorable environments, besides identifying characteristics in the initial growth, to indicate possibility in the increase of growth of the adult plant, favoring the genetic breeding for material more productive. The aim of this research was to study the initial growth of the physic nut (Jatropha curcas L.) in three shading levels (0%, 50% and 90%). The evaluated variables were height of plants, stem base diameter, leaf area, dry mass of the leaves, total dry mass and number of leaves at the 7, 14, 21, 28, 35 and 42 days after the emergency of the plants. The largest height of the plants was observed in 50% of shading and the smallest in 0% of shading. The largest stem base diameter was obtained in plants with 0% of shading and the smallest in 90%. Plants cultivated on 50% of shading presented larger number of leaves, leaf area, dry mass of the leaves and total dry mass. The best development of seedlings of physic nut occurs under 50% of shading in the Gurupi, TO.
    Journal of Biotechnology and Biodiversity. 01/2011; 2(4):43-47.

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