Isolation of Bartonella species from rodents in Taiwan including a strain closely related to 'Bartonella rochalimae' from Rattus norvegicus

Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.
Journal of Medical Microbiology (Impact Factor: 2.25). 01/2009; 57(Pt 12):1496-501. DOI: 10.1099/jmm.0.2008/004671-0
Source: PubMed


An increasing number of Bartonella species originally isolated from small mammals have been identified as emerging human pathogens. During an investigation of Bartonella infection in rodent populations carried out in Taiwan in 2006, a total of 58 rodents were tested. It was determined that 10.3 % (6/58) of the animals were Bartonella bacteraemic. After PCR/RFLP analysis, four isolates were identified as Bartonella elizabethae and one isolate as Bartonella tribocorum. However, there was one specific isolate with an unrecognized PCR/RFLP pattern. After further sequence and phylogenetic analyses of the gltA, ftsZ and rpoB genes, and the 16S-23S rRNA intergenic spacer region, the results indicated that this specific isolate from Rattus norvegicus was closely related to human pathogenic 'Bartonella rochalimae'. Further studies need to be conducted to evaluate whether this rodent species could be a reservoir for 'B. rochalimae'.

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Available from: Bruno Chomel, Oct 07, 2015
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    • "vinsonii (Brenner et al. 1993), B. doshiae, B. grahamii, and B. taylorii (Birtles et al. 1995), B. tribocorum (Heller et al. 1998), B. vinsonii subsp. arupensis (Welch et al. 1999), B. birtlesii (Bermond et al. 2000), B. washoensis (Kosoy et al. 2003), B. phoceensis and B. rattimassiliensis (Gundi et al. 2004), B. rochalimae (Lin et al. 2008), B. tamiae (Kosoy et al. 2008), B. rattaustraliani, B. queenslandensis, and B. coopersplainsensis (Gundi et al. 2009), B. japonica and B. silvatica (Inoue et al. 2010), and B. jaculi, B. callosciuri, B. pachyuromydis , and B. acomydis (Sato et al. 2013). Other Bartonella isolated from rodents have been proposed as new species or subspecies, including Candidatus Bartonella washoensis subsp. "
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    ABSTRACT: Epidemiological studies worldwide have reported a high prevalence and a great diversity of Bartonella species, both in rodents and their flea parasites. The interaction among Bartonella, wild rodents, and fleas reflects a high degree of adaptation among these organisms. Vertical and horizontal efficient Bartonella transmission pathways within flea communities and from fleas to rodents have been documented in competence studies, suggesting that fleas are key players in the transmission of Bartonella to rodents. Exploration of the ecological traits of rodents and their fleas may shed light on the mechanisms used by bartonellae to become established in these organisms. The present review explores the interrelations within the Bartonella-rodent-flea system. The role of the latter two components is emphasized.
    Vector borne and zoonotic diseases (Larchmont, N.Y.) 01/2015; 15(1):27-39. DOI:10.1089/vbz.2014.1606 · 2.30 Impact Factor
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    • "DNA extraction was performed as described earlier. Polymerase chain reaction (PCR) of each individual DNA sample from isolated colonies, animal blood, and ectoparasites was performed with Bartonella-specific primers of the citrate synthase (gltA) gene (Norman et al. 1995), modified as reported previously (Lin et al. 2008). Previously, B. henselae, B. clarridgeiae, Bartonella rattimassiliensis, B. tribocorum, B. grahamii , B. elizabethae, Bartonella phoceensis, and B. rochalimae-like strains have been cultivated in our laboratory. "
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    ABSTRACT: Mainly through vector transmission, domestic cats and dogs are infected by several Bartonella spp. and represent a large reservoir for human infections. This study investigated the relationship of prevalences of Bartonella infection in shelter dogs and cats and various ectoparasite species infesting them (fleas, ticks, and lice). Moreover, relationships between Bartonella infection and animal gender and age and presence of ectoparasites were analyzed. Blood samples were collected from 120 dogs and 103 cats. There were 386 ticks and 36 fleas harvested on these dogs, and 141 fleas, 4 ticks, and 2 lice harvested on these cats. Isolation/detection of Bartonella sp. was performed by culture, polymerase chain reaction (PCR), and partial sequencing. Bartonella was isolated from 21 (20.4%) cats and detected by PCR from 20 (19.4%) cats, 2 (1.7%) dogs, 55 (39%) fleas collected from cats, 28 (10%) ticks DNA samples, and 1 (2.8%) flea collected from dogs. When combining culture and PCR data, 27 cats and 55 fleas collected on cats were positive for Bartonella henselae or Bartonella clarridgeiae, but none were coinfected. Approximately half of the B. henselae isolates from 21 cats were B. henselae type I. Moreover, B. henselae, Bartonella phoceensis, Bartonella queenslandensis, Bartonella rattimassiliensis, Bartonella elizabethae DNA was detected in ticks collected from dogs and one flea was B. clarridgeiae PCR positive. This is the first report of such a wide variety of Bartonella spp. detected in Rhipicephalus sanguineus. Further studies are required to understand the relative importance of these ectoparasites to transmit Bartonella spp. in dogs and cats.
    Vector borne and zoonotic diseases (Larchmont, N.Y.) 12/2010; 11(8):1023-30. DOI:10.1089/vbz.2010.0085 · 2.30 Impact Factor
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    • "Sequence analysis of the amplified fragment (accession number GQ872197) showed a maximum identity of 97% with the rpoB gene of the Bartonella sp. strain 1- 1C isolated from rodents in Taiwan and closely related to Bartonella rochalimae (Lin et al. 2008). "
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    ABSTRACT: In Europe, Ixodes ricinus ticks are vectors of many emerging pathogens, including Borrelia burgdorferi sensu lato (sl), Anaplasma phagocytophilum, spotted fever group Rickettsia sp., Babesia sp., and very likely Bartonella sp. In this study, we looked for the presence of DNA of these microorganisms in 572 ticks from two forests in the west of France. DNA extraction and polymerase chain reaction (PCR) amplification were performed on individual nymphal, male, and female I. ricinus ticks. Amplification from 1 tick among the 572 samples (0.2%) resulted in PCR products with Bartonella-specific primers. Sequence analysis of the amplified fragment did not lead to species identification. Two ticks (0.3%) carried A. phagocytophilum-specific DNA. Eight ticks (1.4%) were positive with spotted fever group Rickettsia-specific primers, and all PCR fragments were related to Rickettsia helvetica. Thirty-five ticks (6.1%) were positive with B. burgdorferi sl-specific primers; the sequences were all related to Borrelia garinii or Borrelia afzelii, except one that was related to Borrelia carolinensis, a newly described species never reported in Europe so far. Thirty-five ticks (6.1%) carried Babesia sp. DNA. Female adults were more infected by B. burgdorferi sl than male adults. The prevalence of B. burgdorferi sl and Babesia sp. was significantly different between the two forests, with a higher prevalence of B. burgdorferi sl in ticks from the forest of Princé and a higher prevalence of Babesia sp. in ticks from the forest of Gâvre. To our knowledge, this is the first study that has detected all five pathogens in questing I. ricinus in the west of France and the first report of B. carolinensis DNA in ticks in Europe.
    Vector borne and zoonotic diseases (Larchmont, N.Y.) 12/2009; 10(8):723-30. DOI:10.1089/vbz.2009.0066 · 2.30 Impact Factor
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