Article
The first metallo-beta-lactamase identified in norway is associated with a TniC-like transposon in a Pseudomonas aeruginosa isolate of sequence type 233 imported from Ghana.
Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway; Department of Microbiology and Virology, IMB, Faculty of Medicine, University of Tromsø, Norway; School of Medicine, Department of Medical Microbiology, Cardiff University, Cardiff, UK; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden; Department of Microbiology, Ullevaal University Hospital, Oslo, Norway.
Antimicrobial Agents and Chemotherapy (impact factor:
4.84).
12/2008;
53(1):331-2.
DOI:10.1128/AAC.00785-08
pp.331-2
Source: PubMed
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Article: I-CeuI reveals conservation of the genome of independent strains of Salmonella typhimurium.
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ABSTRACT: The enzyme I-CeuI, encoded by a class I mobile intron inserted in the gene for 23S rRNA in Chlamydomonas eugamatos, cleaves a specific 19-bp sequence in this gene. This sequence is present only in the seven genes for rRNA in Salmonella typhimurium and Escherichia coli. Partial digestion with I-CeuI of DNA from 17 wild-type strains of S. typhimurium indicates that the chromosome of these strains is strongly conserved, for the digestion products closely resemble those of strain LT2. The lengths and order of chromosomal segments are conserved in 15 of the strains; 2 show some rearrangements. XbaI digestion indicated heterogeneity without revealing the genomic structure. Because of conservation of I-CeuI sites in genes for rRNA and conservation of the number and locations of these genes, I-CeuI provides an excellent tool for the rapid examination of the chromosomes of related species of bacteria; differences in the fingerprints indicate the occurrence of chromosomal rearrangements such as insertions or inversions.Journal of Bacteriology 07/1995; 177(11):3355-7. · 3.83 Impact Factor
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Keywords
Acinetobacter spp
emerging problem
Metallo-beta-lactamases
Pseudomonas aeruginosa
