In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
William Walantus, Laura Elias, Arnold Kriegstein
Institute for Regeneration Medicine, University of California, San Francisco
Correspondence to: William Walantus at firstname.lastname@example.org
Citation: Walantus W., Elias L., Kriegstein A. (2007). In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos. JoVE. 6. http://www.jove.com/index/Details.stp?ID=236,
In-utero in-vivo injection and electroporation of the embryonic rat neocortex provides a powerful tool for the manipulation of individual progenitors
lining the walls of the lateral ventricle. This technique is now widely used to study the processes involved in corticogenesis by over-expressing or
knocking down genes and observing the effects on cellular proliferation, migration, and differentiation. In comparison to traditional knockout
strategies, in-utero electroporation provides a rapid means to manipulate a population of cells during a specific temporal window. In this video
protocol we outline the experimental methodology for preparing rats for surgery, exposing the uterine horns through laporatomy, injecting DNA into
the lateral ventricles of the developing embryo, electroporating DNA into the progenitors lining the lateral wall, and caring for animals post-surgery.
Our laboratory uses this protocol for surgeries on E15-E21 rats, however it is most commonly performed at E16 as shown in this video.
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