Gene Expression Profiling In Chicken Heterophils with Salmonella enteritidis stimulation using a chicken 44K agilent microarray

Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA.
BMC Genomics (Impact Factor: 3.99). 12/2008; 9(1):526. DOI: 10.1186/1471-2164-9-526
Source: PubMed


Salmonella enterica serovar Enteritidis (SE) is one of the most common food-borne pathogens that cause human salmonellosis and usually results from the consumption of contaminated poultry products. The mechanism of SE resistance in chickens remains largely unknown. Previously, heterophils isolated from broilers with different genetic backgrounds (SE-resistant [line A] and -susceptible [line B]) have been shown to be important in defending against SE infections. To dissect the interplay between heterophils and SE infection, we utilized large-scale gene expression profiling.
The results showed more differentially expressed genes were found between different lines than between infection (SE-treated) and non-infection (control) samples within line. However, the numbers of expressed immune-related genes between these two comparisons were dramatically different. More genes related to immune function were down-regulated in line B than line A. The analysis of the immune-related genes indicated that SE infection induced a stronger, up-regulated gene expression of line heterophils A than line B, and these genes include several components in the Toll-like receptor (TLR) signaling pathway, and genes involved in T-helper cell activation.
We found: (1) A divergent expression pattern of immune-related genes between lines of different genetic backgrounds. The higher expression of immune-related genes might be more beneficial to enhance host immunity in the resistant line; (2) a similar TLR regulatory network might exist in both lines, where a possible MyD88-independent pathway may participate in the regulation of host innate immunity; (3) the genes exclusively differentially expressed in line A or line B with SE infection provided strong candidates for further investigating SE resistance and susceptibility. These findings have laid the foundation for future studies of TLR pathway regulation and cellular modulation of SE infection in chickens.

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    • "Microarray technology is a powerful tool which has been used in the chicken to analyze global gene expression profiles during Salmonella infection. Most of the studies pertain to the serovar Enteritidis, some of these studies were performed in vitro on macrophages or cells from the granulosa (Chiang et al., 2008; Ciraci et al., 2010; Tsai et al., 2010; Zhang et al., 2008). Gene expression profiling was studied in vivo on whole organs such as the jejunum (Schokker et al., 2010, 2012; van Hemert et al., 2007), ceca (Higgins et al., 2011) and spleen (Zhou and Lamont, 2007) during the first days of infection. "
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    Veterinary Immunology and Immunopathology 05/2014; 159(1). DOI:10.1016/j.vetimm.2014.03.001 · 1.54 Impact Factor
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    • "While most of the previous studies on lipid accumulation focused on liver, this is the first investigation on adipose tissues providing the groundwork for further understanding of the basic genetic control of growth and development of chicken adipose tissues. (Chiang et al., 2008) 44K Chicken Agilent Oligo microarray Contaminated poultry products are the usual sources of salmonella infection and Salmonella enterica serovar Enteritidis (SE) is a major serotype in many countries. Previous studies on SE resistant and SE susceptible broiler lines have shown that heterophils (a type of leukocyte) play important roles in defence against SE infections. "
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    genesis 05/2013; 51(5). DOI:10.1002/dvg.22387 · 2.02 Impact Factor
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    • "The microarray data were collected and analyzed as described in Chiang et al. (2008). The following linear model was used to contrast infection and control groups: y ijklðkÞ ¼ l þ t i þ r j þ s k þ e ijklðkÞ ; ð1Þ where y ijkl represents the normalized signal contrast intensity with treatment level i (i 5 1 or 2 depending on the contrast direction), replicate j (j 5 1,2,3), array l (l 5 1,2,3,4) in slide k (k 5 1,2); l is an overall mean gene expression; t i is the fixed main effect of treatment (S. enteritidis infection) i; r j is the fixed effect of replicate j; s k is the fixed effect of slide k; and e ijkl is the random error. "
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