Article

The MIRA method for DNA methylation analysis.

Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Methods in Molecular Biology (Impact Factor: 1.29). 02/2009; 507:65-75. DOI: 10.1007/978-1-59745-522-0_6
Source: PubMed

ABSTRACT DNA methylation patterns are often altered in human cancer and aberrant methylation is considered a hallmark of malignant transformation. Several methods have been developed for the characterization of gene-specific and genome-wide DNA methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray platforms to map DNA methylation patterns across the human genome.

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