Flow injection determination of bismuth in urine by successive retention of Bi(III) and tetrahydroborate(III) on an anion-exchange resin and hydride generation atomic absorption spectrometry
University of the Andes (Venezuela), Mérida, Mérida, Venezuela Talanta
(Impact Factor: 3.55).
01/2005; 64(5):1309-16. DOI: 10.1016/j.talanta.2004.05.061
Bismuth as BiCl(4)(-) and BH(4)(-) ware successively retained in a column (150mm x 4mm, length x i.d.) packed with Amberlite IRA-410 (strong anion-exchange resin). This was followed by passage of an injected slug of hydrochloric acid resulting in bismuthine generation (BiH(3)). BiH(3) was stripped from the eluent solution by the addition of a nitrogen flow and the bulk phases were separated in a gas-liquid separator. Finally, bismutine was atomized in a quartz tube for the subsequent detection of bismuth by atomic absorption spectrometry. Different halide complexes of bismuth (namely, BiBr(4)(-), BiI(4)(-) and BiCl(4)(-)) were tested for its pre-concentration, being the chloride complexes which produced the best results. Therefore, a concentration of 0.3moll(-1) of HCl was added to the samples and calibration solutions. A linear response was obtained between the detection limit (3sigma) of 0.225 and 80mugl(-1). The R.S.D.% (n = 10) for a solution containing 50mugl(-1) of Bi was 0.85%. The tolerance of the system to interferences was evaluated by investigating the effect of the following ions: Cu(2+), Co(2+), Ni(2+), Fe(3+), Cd(2+), Pb(2+), Hg(2+), Zn(2+), and Mg(2+). The most severe depression was caused by Hg(2+), which at 60mgl(-1) caused a 5% depression on the signal. For the other cations, concentrations between 1000 and 10,000mgl(-1) could be tolerated. The system was applied to the determination of Bi in urine of patients under therapy with bismuth subcitrate. The recovery of spikes of 5 and 50mugl(-1) of Bi added to the samples prior to digestion with HNO(3) and H(2)O(2) was in satisfactory ranges from 95.0 to 101.0%. The concentrations of bismuth found in six selected samples using this procedure were in good agreement with those obtained by an alternative technique (ETAAS). Finally, the concentration of Bi determined in urine before and after 3 days of treatment were 1.94 +/- 1.26 and 9.02 +/- 5.82mugl(-1), respectively.
Available from: Manuela Leticia Kim
- "Alternatively, the use of a separation or concentration steps in the analytical procedure (i.e., resin columns , imprinted polymers , solid phase extraction/ microextraction ) can be employed. Hydride generation atomic absorption spectrometry (HGAAS) is a good alternative in terms of analytical technique since the analyte is released from the matrix due to formation of a volatile hydride, and several matrix interferences can be overcome through the hydride generation process   . However, specificity and selectivity are often difficult to attain due to the relatively high number of concomitants that can produce and transport volatile species to a given detector. "
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ABSTRACT: A flow injection hydride generation system with a metal furnace atomizer (Inconel 600® alloy) was employed for Bi and Se determination. The presented methods have linear ranges up to 200 and 500 μg L− 1 for Bi and Se, respectively, with good linearities (r2 = 0.9997 and 0.9974, respectively). The limits of quantification obtained according to IUPAC recommendations were 2.3 μg L− 1 for Bi and 6 μg L− 1 for Se, and the relative standard deviations (N = 6) based on Bi and Se analytical responses from real samples were 2.7% and 10%, respectively. Accuracy evaluations were based on certified materials such as SRM 361, SRM 363, and SRM 364 (steel alloys) for Bi, Mess-3 (marine sediment), SRM 397 (human hair), and Bio-Rad2 — 69042 (urine) for Se. Good agreements between the results were obtained at the 95% confidence level, according to the t-test.
Spectrochimica Acta Part B Atomic Spectroscopy 08/2008; 63(8-63):850-855. DOI:10.1016/j.sab.2008.03.012 · 3.18 Impact Factor
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ABSTRACT: This review covers a relatively mature area of atomic spectrometry, hence there are fewer new developments than in other research fields. The review should be read in conjunction with the previous year’s review and with other related reviews in the series. Over the last several years the research emphasis has shifted from novel developments to more routine applications. A plethora of methods have been developed for the preconcentration and separation of analyte and matrix prior to sample introduction. Some novel fundamental studies of plasmas have been published, and new research in the area of laser induced breakdown spectroscopy is still appearing, perhaps reflecting the status of a technique which is always on the verge of “arriving” in the mainstream. The flurry of activity generated by the widespread commercialisation of simultaneous solid-state atomic emission detectors has diminished, particularly for chemometrics and transient signal analysis. The only other major area of novelty is that of speciation analysis using coupled techniques, and even this has moved towards more routine applications.
Journal of Analytical Atomic Spectrometry 05/2005; 20(6):562-590. DOI:10.1039/B505062F · 3.47 Impact Factor
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ABSTRACT: A novel electrochemical hydride generator has been developed for the determination of As and Sb. This newly devised hydride generator is constructed from a flowing electrolytic cell, in which the tungsten wire is selected as cathode. Compared with some cathode material usually used in electrochemical hydride generator, the tungsten cathode is of better interference tolerance, corrosion-resistant and longer working time. The characteristics of the cathode material, hydride generating efficiency and interferences of concomitant have been studied in detail. The detection limits (3sigma) of As and Sb in sample solution were 0.10mugL(-1) and 0.15mugL(-1), the precisions for 11 replicate measurements of 20mugL(-1) As and Sb were 1.3% and 1.7%. The electrochemical hydride generator coupled with atomic fluorescence spectrometry has been applied to the determination of total As and Sb in tobacco samples.
Talanta 03/2006; 68(4):1316-21. DOI:10.1016/j.talanta.2005.07.041 · 3.55 Impact Factor
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