Antiviral Activity of Some Plants Used in Nepalese Traditional Medicine

Research Center for Applied Science and Technology, Tribhuvan University, Kathmandu, Nepal.
Evidence-based Complementary and Alternative Medicine (Impact Factor: 1.88). 11/2007; 6(4):517-22. DOI: 10.1093/ecam/nem156
Source: PubMed

ABSTRACT Methanolic extracts of 41 plant species belonging to 27 families used in the traditional medicine in Nepal have been investigated for in vitro antiviral activity against Herpes simplex virus type 1 (HSV-1) and influenza virus A by dye uptake assay in the systems HSV-1/Vero cells and influenza virus A/MDCK cells. The extracts of Astilbe rivularis, Bergenia ciliata, Cassiope fastigiata and Thymus linearis showed potent anti-herpes viral activity. The extracts of Allium oreoprasum, Androsace strigilosa, Asparagus filicinus, Astilbe rivularis, Bergenia ciliata and Verbascum thapsus exhibited strong anti-influenza viral activity. Only the extracts of A. rivularis and B. ciliata demonstrated remarkable activity against both viruses.

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Available from: Mohan Gewali, Mar 27, 2014
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    • "On the other hand, combination of adamantanamine glucuronide and the lyophilized flower infusion of V. thapsiforme exerted a discernible effect against influenza virus in chicken embryo Fig. 14 Other compounds isolated from Verbascum spp fibroblast cell cultures (Serkedjieva 2000). In connection with these data, the methanolic extract of V. thapsus from Nepal was revealed to have a robust antiinfluenza effect (Rajbhandari et al. 2009). "
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    ABSTRACT: The genus Verbascum (mulleins), belonging to the family Scrophulariaceae, comprises about 360 species of flowering plants. The leaves, flowers and whole aerial parts of Verbascum spp have been widely used in traditional medicine for the treatment of respiratory and inflammatory disorders and also display powerful wound healing activity. Verbascum species are found to accumulate several groups of bioactive molecules, therefore they might be utilized as attractive sources of new (drug) leads. The present review attempts to provide an up-to-date comprehensive overview on phytochemical and pharmacological aspects of Verbacum spp research along with some successful examples of growing (and transforming) mulleins in vitro.
    Phytochemistry Reviews 04/2014; DOI:10.1007/s11101-014-9361-5 · 2.41 Impact Factor
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    • "Investigation was for in vitro antiviral activity against Herpes simplex virus type 1 (HSV-1) and influenza virus A by dye uptake assay systems HSV-1/Vero in the cells and influenza virus A/MDCK cells.V. thapsus exhibited strong anti-influenza virus activity with IC 50 < 6.25 mg/ml (Rajbhandari et al., 2009). "
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    ABSTRACT: Verbascum thapsus L. [Khardhag or Common mullein], a member of the family Scrophulariaceae, is a famous herb that is found all over Europe, in temperate Asia, in North America and is well-reputed due to its medicinal properties. This medicinal herb contains various chemical constituents like saponins, iridoid and phenylethanoid glycosides, flavonoids, vitamin C and minerals. It is famous in various communities worldwide for the treatment of various disorders of both humans and animals aliments. A number of pharmacological activities such as anti-inflammatory, antioxidant, anticancer, antimicrobial, antiviral, antihepatotoxic and anti-hyperlipidemic activity have been ascribed to this plant. The plant is used to treat tuberculosis also, earache and bronchitis. In the present paper botanical and ethnomedicinal description, pharmacological profile and phytochemistry of this herb is being discussed.
    Revista Brasileira de Farmacognosia 11/2013; 23(6):948–959. DOI:10.1590/S0102-695X2013000600012 · 0.83 Impact Factor
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    • "Many natural compounds have been examined for their antiviral or antiinflammatory effects during influenza A virus infection, and the active components in many cases have been isolated. Some of these include the traditional Chinese medicines Evodia rutaecarpa [25] and Sophora flavescens [26], as well as several plants commonly used in Nepalese traditional medicine [27]. "
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    ABSTRACT: Ekybion is a drug complex of 16 natural extracts and inorganic compounds designed to treat a variety of respiratory pathogens of bacterial and viral origin. It is licensed throughout Europe for the treatment of respiratory tract infections from equine parainfluenza type 3 and equine herpes virus type 1 in equine stables. The purpose of this paper was to test the efficacy of Ekybion on a well-developed animal model of influenza A infection and determine a mode of action. Experiments were performed with Balb/c mice infected with a lethal dose of influenza A/PR/8/34 H1N1 virus and treated with nebulized Ekybion every 8 h in a time-dependant or dose-dependant fashion. These experiments showed that mice treated prior to infection with Ekybion had a higher survival rates (~46%) compared with untreated animals (~0%). Paradoxically, these mice showed no significant difference in lung virus titer or weight loss. There was, however, a decrease in the level of GM-CSF, IL-6, and G-CSF cytokines in the lungs of Ekybion-treated, infected mice. It is possible that decreases in proinflammatory cytokines may have contributed to increased survivorship in Ekybion-treated influenza-infected mice.
    Evidence-based Complementary and Alternative Medicine 01/2011; 2011(1741-427X):192079. DOI:10.1155/2011/192079 · 1.88 Impact Factor
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