Climacteric or non-climacteric behavior in melon fruit 2. Linking climacteric pattern and main postharvest disorders and decay in a set of near-isogenic lines

IRTA, Centre de Recerca en Agrigenòmica (CSIC-IRTA-UAB), Carretera de Cabrils Km 2, E-08348 Cabrils (Barcelona), Spain
Postharvest Biology and Technology (Impact Factor: 2.22). 11/2008; 50(2-3):125. DOI: 10.1016/j.postharvbio.2008.04.007


A set of near-isogenic lines (NILs) of melon (Cucumis melo L.) was used to test the relationship between the climacteric pattern and postharvest disorders at harvest and after 30 days at 8 °C. The NILs contained different chromosome introgressions in the linkage group III from the non-climacteric exotic Korean accession PI 161375 transferred into the genetic background of the non-climacteric Spanish cultivar ‘Piel de Sapo’ (PS). A quantitative trait locus (QTL) in this linkage group induced climacteric behavior in eight NILs accompanied by a peak of ethylene production and fruit dehiscence to different degrees. The cultivar ‘Nicolás’ and one NIL showed a non-climacteric pattern of respiration rate and ethylene production. The climacteric NILs were used to test the relationship between this pattern and postharvest disorders. The reference climacteric lines ‘Fado’ and ‘Védrantais’ were more sensitive to CI and associated Cladosporium rot than the NILs or PS. In general, a more intense climacteric behavior was accompanied by fruit dehiscence, and higher total losses and greater skin scald after storage, than in PS. A higher incidence of chilling injury (CI) in the climacteric NILs was found compared with the non-climacteric ones, although with exceptions (one NIL for CI in the form of scald; the same NIL and one more for pitting). The climacteric onset and netting scald were not related, and CI in the form of skin spots was only found in climacteric NILs and was positively correlated with the maximum peak of ethylene production. Some climacteric NILs did not follow the rule of a higher susceptibility to other disorders and decay after storage compared with PS, such as for example in fruit over-ripening (detected externally or internally), Cladosporium rot at the peduncle and Alternaria rot. Mealiness was independent of climacteric behavior. Three climacteric NILs obtained better flavor scores after storage than PS, although the maximum peak of ethylene production was positively correlated with off-flavor. Genotypic correlation between disorder data and the physiological data of climacteric fruit revealed positive (flavor index) or negative postharvest consequences (skin injuries, rots or off-flavors). At least one QTL can be assigned to most of the quality traits analyzed.

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    • "Según los niveles de producción de etileno y de la tasa respiratoria, datos no mostrados o publicados en [6] [11], las líneas se clasificaron en: No climatérica (PS y " Nicolás " ), climatérica leve (6M1) y climatérica moderada ( " Vedrantais " ). "

    • "Ethylene dependent and independent ripening pathways coexist in melon (Flores et al., 2001; 2002; Obando-Ulloa et al., 2009c). Ethylene regulates processes such as biosynthesis of aromatic compounds, rind color changes, most parts of fruit softening, sensitivity to chilling injury, and other aspects of postharvest shelf life (Ayub et al., 1996; Flores et al., 2001; 2002; El-Sharkawy et al., 2005; Fernández-Trujillo et al., 2008; Obando-Ulloa et al., 2008; 2009a; 2009c; Pech et al., 2008a). Other processes such as sugar accumulation, loss of acidity, coloration of the pulp, and parts of fruit softening are ethlylene-independent (Ayub et al., 1996, Pech et al., 2008a). "
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    ABSTRACT: Indian melon (Cucumis melo L.) landraces comprise a wealth of genetic diversity that has been exploited over the millennia by farmers and over the last century by scientifically trained plant scientists in the public and private sectors. Melons in India may be feral or cultivated, have netted or smooth rinds, be sweet and eaten as a dessert fruit or not sweet and consumed as a vegetable fresh, cooked, or dried. The fruit may be processed for sweet juice and confectionary flavoring, and the seeds are a source of high-quality cooking oil and high-protein seed meal. This chapter reviews genetic variation for resistance to fungal, bacterial, and viral diseases and to nematodes and insects; and tolerance to soil salinity, drought, flooding, and high temperatures with a focus on melon accessions of Indian origins. Some of these resistances have knowing or unknowingly been transferred through scientifically based breeding programs into open-pollinated and hybrid sweet melon cultivars grown in Africa, Asia, Australia, Europe, and the Americas for domestic and export markets. Indian melons include unique sources of high acidity and sugar:acid ratio that enable breeding for new combinations of sweet and sour flavors for fresh and processed melon products. Genetic variation in carotenoids (β-carotene), ascorbic acid, and micronutrient (Fe and Zn) contents in Indian germplasm promise more nutritious melons. High percentages of unique alleles are present in southern (24.2%)andeastern (30.4%) Indian landraces and in "wild" accessions from northern India (34.5%). Extensive collection, preservation, and evaluation of Indian melon landraces is vital to prevent further genetic erosion in this primary center of melon diversity, to increase genetic variability for melon breeding, and to introduce new traits into modern melon cultivars. International collaborations are developing genomics tools for melon that will facilitate allele mining within Indian germplasm and introduce new genetic variability.We are on the verge of an exciting era of melon genetic improvement as whole-plant breeding and genomics technologies combine to preserve and fully characterize the complete array of genetic variability in melon and exploit that germplasm and information for the further improvement of salad and dessert melons for diverse markets worldwide.
    Plant Breeding Reviews, Volume 35, 10/2011: pages 85 - 150; , ISBN: 9781118100509
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    • "The NILs SC3-5, 5M1–5M10 and 6M1–6M9 contained introgressions of different extents from the Korean accession 'Shongwan Charmi' PI161375 (SC) on the linkage group III into the 'Piel de Sapo' (PS) genetic background (Eduardo et al., 2005; Fernández-Trujillo et al., 2008; Moreno et al., 2008). SC10-2 and SC7-1 (Eduardo et al., 2005) contained introgressions of SC into PS in linkage groups X and VII, respectively. "
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    ABSTRACT: In two consecutive seasons the firmness of 13¿15 near-isogenic lines (NILs) of melons (Cucumis melo L.) was followed during storage at 21 °C. Firmness was measured using non-destructive compression of whole melon fruit to a predefined compression distance of 2 mm. The same individuals (about 6 per near-isogenic line) were repeatedly measured over time. Integral statistical analysis of all individuals using non-linear mixed effects regression analysis revealed that the rate constant of the exponential firmness decrease was the same for all NILs irrespective of their differences in introgression in linkage III or in the other two linkage groups. The only difference observed was found in the (asymptotic) end value of softening. That would imply that the process of softening is very similar, although over a different range for each melon. Melons from some NILs were firmer and showed a higher end value of softening than those of other NILs. The percentage variance accounted for () was 94% (523 observations) for the 2005 season and 85% (829 observations) for the 2006 season. A small variation in asymptotic end value together with a low end value as to ascertain edibility could be a good indication of the usefulness of certain NILs for commercial application.
    Postharvest Biology and Technology 03/2009; 51:320-326. DOI:10.1016/j.postharvbio.2008.06.001 · 2.22 Impact Factor
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