Effects of developmental exposure to bisphenol A on brain and behavior in mice
ABSTRACT Bisphenol A (BPA) is a widespread estrogenic chemical used in the production of polycarbonate, and epoxy resins lining food and beverage cans and in dental sealants. During fetal life the intrauterine environment is critical for the normal development, and even small changes in the levels of hormones, such as estradiol or estrogen-mimicking chemicals, can lead to changes in brain function and consequently in behavior. We review here a series of ethological studies on the effects of maternal oral exposure during the last part of gestation (prenatal exposure) or from gestation day 11 to postnatal day 7 (perinatal exposure) to a low, environmentally relevant dose of BPA (10 microg/kg bw/day) on behavioral responses of CD-1 mouse offspring. We examined both male and female offspring and found that maternal exposure to BPA affected: (1) behavioral responses to novelty before puberty and, as adults; (2) exploration and activity in a free-exploratory open field; (3) exploration in the elevated plus maze and (4) sensitivity to amphetamine-induced reward in the conditioned place preference test. A consistent effect of the maternal exposure to BPA is that in all these different experimental settings, while a significant sex difference was observed in the control group, exposure to BPA decreased or eliminated the sex difference in behavior. In addition, exposure of female mice to BPA in both adulthood or during fetal life altered subsequent maternal behavior. These findings, together with those from other laboratories, are evidence of long-term consequences of maternal exposure to low-dose BPA at the level of neurobehavioral development.
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ABSTRACT: Developmental bisphenol A (BPA) exposure is associated with adverse behavioral effects, although underlying modes of action remain unclear. Because BPA is a suspected xenoestrogen, the objective was to identify sex-based changes in adult zebrafish social behavior developmentally exposed to BPA (0.0, 0.1, or 1 μM) or one of two control compounds (0.1 μM 17β-estradiol [E2], and 0.1 μM GSK4716, a synthetic estrogen-related receptor γ ligand). A test chamber was divided lengthwise so each arena held one fish unable to detect the presence of the other fish. A mirror was inserted at one end of each arena; baseline activity levels were determined without mirror. Arenas were divided into three computer-generated zones to represent different distances from mirror image. Circadian rhythm patterns were evaluated at 1-3 (= AM) and 5-8 (= PM) h postprandial. Adult zebrafish were placed into arenas and monitored by digital camera for 5 min. Total distance traveled, percent of time spent at mirror image, and number of attacks on mirror image were quantified. E2, GSK4716, and all BPA treatments dampened male activity and altered male circadian activity patterns; there was no marked effect on female activity. BPA induced nonmonotonic effects (response curve changes direction within range of concentrations examined) on male percent of time at mirror only in AM. All treatments produced increased percent of time at the mirror during PM. Male attacks on the mirror were reduced by BPA exposure only during AM. There were sex-specific effects of developmental BPA on social interactions, and time of day of observation affected results.Journal of Toxicology and Environmental Health Part A 01/2015; 78(1):50-66. DOI:10.1080/15287394.2015.958419 · 1.83 Impact Factor
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ABSTRACT: A simple and label-free electrochemical aptasensor for bisphenol A (BPA) determination was developed based on gold nanoparticles dotted graphene (GNPs/GR) nanocomposite film modified glassy carbon electrode (GCE). The electrochemical probe of ferricyanide was used to investigate the interactions between aptamer and BPA. The resulting GNPs/GR layer exhibited good current response for BPA detection. The highly conductive and biocompatible nanostructure of GNPs/GR nanocomposite was characterised by atomic force microscope (AFM), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The peak current change (ΔI) of ferricyanide was linear with the concentration of BPA in the range from 0.01 μM to 10 μM with the detection limit of 5 nM. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of BPA. Particularly, the aptasensor was applied successfully to determine BPA in milk products, and the average recovery was 105%.Food Chemistry 11/2014; 162:34–40. DOI:10.1016/j.foodchem.2014.04.058 · 3.26 Impact Factor
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ABSTRACT: Perinatal life is a critical window for sexually dimorphic brain organization, and profoundly influenced by steroid hormones. Exposure to endocrine disrupting compounds (EDCs) may disrupt this process, resulting in compromised reproductive physiology and behavior. To test the hypothesis that neonatal BPA exposure can alter sex specific postnatal ER expression we first mapped ER mRNA levels in the principal nucleus of the bed nucleus of the stria terminalis (BNSTp), paraventricular nucleus (PVN), anterior portion of the medial amygdaloid nucleus (MeA), super optic nucleus (SON), suprachiasmic nucleus (SCN) and lateral habenula (LHb) across postnatal days (PNDs) 0 to 19. Next, rat pups of both sexes were subcutaneously injected over the first three days of life with 10 g estradiol benzoate (EB), 50 g/kg BPA (LBPA), or 50 mg/kg BPA (HBPA) and ERβ levels were quantified in each region of interest (ROI) on PNDs 4 and 10. EB exposure decreased ER signal in most female ROIs, and in the male PVN. In the BNSTp, ER expression decreased in LBPA males and HBPA females on PND 10, thereby reversing the sex difference in expression. In the PVN, ER mRNA levels were elevated in LBPA females, also resulting in a reversal of sexually dimorphic expression. In the MeA, BPA decreased ERβ expression on PND 4. Collectively, these data demonstrate that region and sex specific ER expression is vulnerable to neonatal BPA exposure in the developing rat brain.Reproduction 12/2013; 147(4). DOI:10.1530/REP-13-0501