LRP-6 is co-receptor for multiple fibrogenic pathways in pericytes and myofibroblasts that are inhibited by DKK-1
ABSTRACT Fibrosis of vital organs is a major public health problem with limited therapeutic options. Mesenchymal cells including microvascular mural cells (pericytes) are major progenitors of scar-forming myofibroblasts in kidney and other organs. Here we show pericytes in healthy kidneys have active WNT/β-catenin signaling responses that are markedly up-regulated following kidney injury. Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation. DKK-1 blocks activation and proliferation of established myofibroblasts in vitro and blocks pericyte proliferation to PDGF, pericyte migration, gene activation, and cytoskeletal reorganization to TGF-β or connective tissue growth factor. These effects are largely independent of inhibition of downstream β-catenin signaling. DKK-1 acts predominantly by inhibiting PDGF-, TGF-β-, and connective tissue growth factor-activated MAPK and JNK signaling cascades, acting via LRP-6 with associated WNT ligand. Biochemically, LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin. In summary, DKK-1 blocks many of the changes in pericytes required for myofibroblast transition and attenuates established myofibroblast proliferation/activation by mechanisms dependent on LRP-6 and WNT ligands but not the downstream β-catenin pathway.
- SourceAvailable from: Shixin Tao
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- "Renal β-catenin is increased in experimental models of renal fibrosis (He et al., 2009; Ren et al., 2013; Surendran et al., 2005) and its systemic inhibition can reduce fibrosis (Hao et al., 2011). However, although DKK-1, a Wnt antagonist significantly reduced renal β-catenin abundance (He et al., 2009), the anti-fibrotic effects of DKK-1 were found to be independent of β-catenin in UUO and I/R models of fibrosis (Ren et al., 2013). Moreover, gene deletion of β-catenin in tubular epithelium did not reduce renal fibrosis (Zhou et al., 2013). "
ABSTRACT: Glycogen synthase kinase-3β (GSK3β) is a serine/threonine protein kinase that plays an important role in renal tubular injury and regeneration in acute kidney injury. However its role in the development of renal fibrosis, often a long-term consequence of acute kidney injury is unknown. Using a mouse model of renal fibrosis induced by ischemia/reperfusion injury, we demonstrate increased GSK3β expression and activity in fibrotic kidneys and its presence in myofibroblasts in addition to tubular epithelial cells. Pharmacological inhibition of GSK3 using TDZD-8 starting before or after ischemia/reperfusion significantly suppressed renal fibrosis by reducing myofibroblast population, collagen-1 and fibronectin deposition, inflammatory cytokines and macrophage infiltration. GSK3 inhibition in vivo reduced TGF-β1, SMAD3 activation and plasminogen activator inhibitor-1 levels. Consistently in vitro, TGF-β1 treatment increased GSK3β expression and GSK3 inhibition abolished TGF-β1 induced SMAD3 activation and α-smooth muscle actin expression in cultured renal fibroblasts. Importantly, overexpression of constitutively active GSK3β stimulated α-smooth muscle actin expression even in the absence of TGF-β1 treatment. These results suggest that TGF-β regulates GSK3β, which in turn is important for TGF-β/SMAD3 signaling and fibroblast-to-myofibroblast differentiation. Overall these studies demonstrate that GSK3 could promote renal fibrosis by activation of TGF-β signaling and the use of GSK3 inhibitors might represent a novel therapeutic approach for progressive renal fibrosis that develops as a consequence of acute kidney injury. © 2015. Published by The Company of Biologists Ltd.Disease Models and Mechanisms 06/2015; 8(8). DOI:10.1242/dmm.020511 · 5.54 Impact Factor
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- "Wnt/β-catenin signaling modulates the recruitment and resolution of inflammatory cells Since Wnt/β-catenin signaling is required for blastema formation and regenerative outgrowth in zebrafish caudal fins (Ito et al., 2007; Kawakami et al., 2006; Poss et al., 2000; Stoick-Cooper et al., 2007a,b), but also modulates inflammatory processes including scar formation, fibrosis, wound healing and tissue remodeling in mammals (French et al., 2004; Ren et al., 2013; Koch et al., 2011), we investigated whether there might be a role for Wnt/β-catenin signaling in regulating inflammation during fin regeneration. "
ABSTRACT: Neutrophils and macrophages, as key mediators of inflammation, have defined functionally important roles in mammalian tissue repair. Although recent evidence suggests that similar cells exist in zebrafish and also migrate to sites of injury in larvae, whether these cells are functionally important for wound healing or regeneration in adult zebrafish is unknown. To begin to address these questions, we first tracked neutrophils (lyzC(+), mpo(+)) and macrophages (mpeg1(+)) in adult zebrafish following amputation of the tail fin, and detailed a migratory timecourse that revealed conserved elements of the inflammatory cell response with mammals. Next, we used transgenic zebrafish in which we could selectively ablate macrophages, which allowed us to investigate whether macrophages were required for tail fin regeneration. We identified stage-dependent functional roles of macrophages in mediating fin tissue outgrowth and bony ray patterning, in part through modulating levels of blastema proliferation. Moreover, we also sought to detail molecular regulators of inflammation in adult zebrafish and identified Wnt/β-catenin as a signaling pathway that regulates the injury microenvironment, inflammatory cell migration and macrophage phenotype. These results provide a cellular and molecular link between components of the inflammation response and regeneration in adult zebrafish.Development 07/2014; 141(13):2581-91. DOI:10.1242/dev.098459 · 6.27 Impact Factor
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ABSTRACT: ABSTRACT Chronic kidney disease (CKD) defined as loss of kidney function for more than three months, is characterized pathologically by glomerulosclerosis, interstitial fibrosis, tubular atrophy, peritubular capillary rarefaction and inflammation. Recent studies have identified a previously poorly-appreciated, yet extensive population of mesenchymal cells, called either pericytes when attached to peri-tubular capillaries or resident fibroblasts when embedded in matrix, as the progenitors of scar-forming cells known myofibroblasts. In response to sustained kidney injury, pericytes detach from the vasculature and differentiate into myofibroblasts, a process not only causing fibrosis, but also directly contributing to capillary rarefaction and inflammation. The inter-relationship of these three detrimental processes makes myofibroblasts and their pericyte progenitors an attractive target in chronic kidney disease. In this review we will describe current understanding of the mechanisms of pericyte to myofibroblast differentiation during chronic kidney disease, draw parallels with disease processes in the glomerulus, and highlight promising new therapeutic strategies that target pericytes or myofibroblasts. In addition we will describe the critical paracrine roles of epithelial, endothelial and innate immune cells in the fibrogenic process.AJP Cell Physiology 01/2013; 304(7). DOI:10.1152/ajpcell.00414.2012 · 3.67 Impact Factor