Article

Genetic Characterization of Sapovirus Detected in Hospitalized Children with Acute Gastroenteritis in Korea

Seoul Metropolitan Government Research Institute of Public Health and Environment, Virus team, 202-3, Yangjae-Dong, Seocho-Gu, Seoul 137-734, Republic of Korea.
Clinical laboratory (Impact Factor: 1.08). 01/2012; 58(11-12):1219-24. DOI: 10.7754/Clin.Lab.2012.120118
Source: PubMed

ABSTRACT Human Caliciviruses, including sapovirus, are important causes of gastroenteritis in children and adults. The present study determined the detection rate of sapovirus (SaV) with acute gastroenteritis in hospitalized children and describes the molecular epidemiology of SaV circulating in Seoul, Korea.
In total, 4,583 stool specimens from hospitalized patients with acute gastroenteritis were collected (2,058 females and 2,525 males) in Seoul and were tested for SaVs.
SaV GI was classified further into two genotypes and GI-1 strains were responsible for two of the cases and GI-2 constituted a further three of the SaV gastroenteritis cases in this study. A phylogenetic analysis of these SaV cases revealed that the GI-1 and GI-2 strains tend to be closely associated with each other and were classified into Asian and European strains. This is the first molecular characterization report of SaV detected in Korea. The GI-1 and GI-2 strains were detected from hospitalized children with acute gastroenteritis.

0 Followers
 · 
71 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Sapoviruses cause acute gastroenteritis in humans and animals. They belong to the genus Sapovirus within the family Caliciviridae. They infect and cause disease in humans of all ages, in both sporadic cases and outbreaks. The clinical symptoms of sapovirus gastroenteritis are indistinguishable from those caused by noroviruses, so laboratory diagnosis is essential to identify the pathogen. Sapoviruses are highly diverse genetically and antigenically. Currently, reverse transcription-PCR (RT-PCR) assays are widely used for sapovirus detection from clinical specimens due to their high sensitivity and broad reactivity as well as the lack of sensitive assays for antigen detection or cell culture systems for the detection of infectious viruses. Sapoviruses were first discovered in 1976 by electron microscopy in diarrheic samples of humans. To date, sapoviruses have also been detected from several animals: pigs, mink, dogs, sea lions, and bats. In this review, we focus on genomic and antigenic features, molecular typing/classification, detection methods, and clinical and epidemiological profiles of human sapoviruses. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    Clinical Microbiology Reviews 01/2015; 28(1):32-53. DOI:10.1128/CMR.00011-14 · 16.00 Impact Factor