Human Caliciviruses, including sapovirus, are important causes of gastroenteritis in children and adults. The present study determined the detection rate of sapovirus (SaV) with acute gastroenteritis in hospitalized children and describes the molecular epidemiology of SaV circulating in Seoul, Korea.
In total, 4,583 stool specimens from hospitalized patients with acute gastroenteritis were collected (2,058 females and 2,525 males) in Seoul and were tested for SaVs.
SaV GI was classified further into two genotypes and GI-1 strains were responsible for two of the cases and GI-2 constituted a further three of the SaV gastroenteritis cases in this study. A phylogenetic analysis of these SaV cases revealed that the GI-1 and GI-2 strains tend to be closely associated with each other and were classified into Asian and European strains. This is the first molecular characterization report of SaV detected in Korea. The GI-1 and GI-2 strains were detected from hospitalized children with acute gastroenteritis.
[Show abstract][Hide abstract] ABSTRACT: Sapovirus (SaV), a virus residing in the intestines, is one of the important causes of gastroenteritis in human beings. Human SaV genomes are classified into various genogroups and genotypes. Whole-genome analysis and phylogenetic analysis of ROK62, the SaV isolated in South Korea, were carried out. The ROK62 genome of 7429 nucleotides contains 3 open-reading frames (ORF). The genotype of ROK62 is SaV GI-1, and 94% of its nucleotide sequence is identical with other SaVs, namely Manchester and Mc114. Recently, SaV infection has been on the rise throughout the world, particularly in countries neighboring South Korea; however, very few academic studies have been done nationally. As the first whole-genome sequence analysis of SaV in South Korea, this research will help provide reference for the detection of recombination, tracking of epidemic spread, and development of diagnosis methods for SaV.
PLoS ONE 07/2015; 10(7):e0132328. DOI:10.1371/journal.pone.0132328 · 3.23 Impact Factor
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