Nanoprisms: Gold Nanoprisms as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Gastrointestinal Cancers (Small 1/2013)

Department of Bio-Nano Science and Engineering, National Key Laboratory of Micro/Nano, Fabrication Technology, Institute of Micro & Nano Science and Technology, Shanghai JiaoTong University, Shanghai, 200240, China.
Small (Impact Factor: 8.37). 01/2013; 9(1):67. DOI: 10.1002/smll.201370007
Source: PubMed


On page 68, PEGylated gold nanoprisms are designed and prepared by J. M. de la Fuente, D. Cui, and co-workers, with the aim to study the feasibility of using them as a novel contrast agent for the hybrid technique of optoacoustic imaging. The nanoprisms are imaged at different scales using different imaging modalities. They are confirmed as biocompatible, and selected colon cancer HT-29 cells are used as research targets. Shown here is an in silico electron tomographic reconstruction of such gold nanostructures, which show promise for application in biomedical imaging, drug delivery, and photothermal therapy.

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Available from: Chenchen Bao, Oct 01, 2015
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    • "a useful tool for a number of biomedical applications such as drug delivery (Han et al. 2007), light-triggered apoptosis (Pérez-Hernández et al. 2015), optoacustic imaging (Bao et al. 2013), and thermal and optical sensing of biomarkers (Howes et al. 2014; Polo et al. 2013). Among the different nanostructures reported in the literature, anisotropic nanoparticles and particularly gold nanorods (AuNR) present several advantages in comparison with more conventional spherical nanoparticles . "
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    ABSTRACT: In this article, we describe how it is possible to tune the size and the aspect ratio of gold nanorods obtained using a highly efficient protocol based on the use of hydroquinone as a reducing agent by varying the amounts of CTAB and silver ions present in the “seed-growth” solution. Our approach not only allows us to prepare nanorods with a four times increased Au3+ reduction yield, when compared with the commonly used protocol based on ascorbic acid, but also allows a remarkable reduction of 50–60 % of the amount of CTAB needed. In fact, according to our findings, the concentration of CTAB present in the seed-growth solution do not linearly influence the final aspect ratio of the obtained nanorods, and an optimal concentration range between 30 and 50 mM has been identified as the one that is able to generate particles with more elongated shapes. On the optimized protocol, the effect of the concentration of Ag+ ions in the seed-growth solution and the stability of the obtained particles has also been investigated.
    Journal of Nanoparticle Research 08/2015; 17(8). DOI:10.1007/s11051-015-3136-9 · 2.18 Impact Factor
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    • "Organic fluorochromes, while preferred for labelling in preclinical and clinical optical imaging, are often not ideal as they typically lose their light absorbing properties following light exposure. We have recently developed a powerful in vivo MSOT contrast agent by incorporating the strong photo-absorbing probe indocyanine green (ICG) into PEGylated liposomes (Beziere et al., 2015), demonstrating enhanced optoacoustic imaging characteristics compared to gold nanorods (Bao et al., 2013; Herzog et al., 2012; Lozano et al., 2012) for the same number of nanoparticles injected. "
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    ABSTRACT: Indocyanine green (ICG) is an FDA-approved, strongly photo-absorbent/fluorescent probe that has been incorporated into a clinically-relevant PEGylated liposome as a flexible optoacoustic contrast agent platform. This study describes the engineering of targeted PEGylated liposome-ICG using the anti-MUC-1 "humanized" monoclonal antibody (MoAb) hCTM01 as a tumour-specific theranostic system. We aimed to visualise non-invasively the tumour accumulation of these MoAb-targeted liposomes over time in tumour-bearing mice using multispectral optoacoustic tomography (MSOT). Preferential accumulation of targeted PEGylated liposome-ICG was studied after intravenous administration in comparison to non-targeted PEGylated liposome-ICG using both fast growing (4T1) and slow growing (HT-29) MUC-1 positive tumour models. Monitoring liposomal ICG in the tumour showed that both targeted and non-targeted liposome-ICG formulations preferentially accumulated into the tumour models studied. Rapid accumulation was observed for targeted liposomes at early time points mainly in the periphery of the tumour volume suggesting binding to available MUC-1 receptors. In contrast, non-targeted PEGylated liposomes showed accumulation at the centre of the tumour at later time points. In an attempt to take this a step further, we successfully encapsulated the anticancer drug, doxorubicin (DOX) into both targeted and non-targeted PEGylated liposome-ICG. The engineering of DOX-loaded targeted ICG liposome systems present a novel platform for combined tumour-specific therapy and diagnosis. This can open new possibilities in the design of advanced image-guided cancer therapeutics. Copyright © 2014 Elsevier B.V. All rights reserved.
    International Journal of Pharmaceutics 10/2014; 482(1-2). DOI:10.1016/j.ijpharm.2014.10.045 · 3.65 Impact Factor
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    • "Gold nanorods (GNRs), because of their small size, strong light-enhanced absorption in the NIR, and plasmon resonance-enhanced properties, have become attractive noble nanomaterials for their potential in applications such as photothermal therapy [37], biosensing [38], PA imaging [39], and gene delivery [40] for cancer treatment. However, the toxicity derived from a large amount of the surfactant cetyltrimethylammonium bromide (CTAB) during GNR synthesis severely limits their biomedical applications. "
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    ABSTRACT: Herein, we reported for the first time that RGD-conjugated silica-coated gold nanorods on the surface of multiwalled carbon nanotubes were successfully used for targeted photoacoustic imaging of in vivo gastric cancer cells. A simple strategy was used to attach covalently silica-coated gold nanorods (sGNRs) onto the surface of multiwalled carbon nanotubes (MWNTs) to fabricate a hybrid nanostructure. The cross-linked reaction occurred through the combination of carboxyl groups on the MWNTs and the amino group on the surface of sGNRs modified with a silane coupling agent. RGD peptides were conjugated with the sGNR/MWNT nanostructure; resultant RGD-conjugated sGNR/MWNT probes were investigated for their influences on viability of MGC803 and GES-1 cells. The nude mice models loaded with gastric cancer cells were prepared, the RGD-conjugated sGNR/MWNT probes were injected into gastric cancer-bearing nude mice models via the tail vein, and the nude mice were observed by an optoacoustic imaging system. Results showed that RGD-conjugated sGNR/MWNT probes showed good water solubility and low cellular toxicity, could target in vivo gastric cancer cells, and obtained strong photoacoustic imaging in the nude model. RGD-conjugated sGNR/MWNT probes will own great potential in applications such as targeted photoacoustic imaging and photothermal therapy in the near future.
    Nanoscale Research Letters 05/2014; 9(1):264. DOI:10.1186/1556-276X-9-264 · 2.78 Impact Factor
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