An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry. Environ Monit Assess

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.
Environmental Monitoring and Assessment (Impact Factor: 1.68). 11/2008; 158(1-4):35-49. DOI: 10.1007/s10661-008-0563-4
Source: PubMed


A procedure using pre-column trimethylsilyl derivatization and gas chromatography/ mass spectrometry (GC/MS) was developed and applied in determining trace estrogens in complex matrix. Main conditions were optimized, including pH value, salinity of water sample, elution reagents, clean procedure, derivative solvent and temperature. The optimized method was used to determine steroid estrogens in surface water and effluents of wastewater treatment plant (WWTP). Low detection limits of 0.01, 0.03, 0.03, 0.07, 0.09 and 0.13 ng/l for DES, E1, E2, EE2, E3 and E(V), respectively were obtained under optimism condition. No apparent interferences appeared in chromatography in comparison with ultrapure water blank. Mean recovery ranged from 72.6% to 111.0% with relative standard deviation of 1.1-4.6% for spiked surface water, and from 66.6% to 121.1% with relative standard deviation of 1.5-4.7% for spiked effluent of WWTP. The results suggested that the optimized method provides a robust solution for the determination of trace steroid estrogens in complex matrix.

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Available from: Yiqi Zhou, Nov 19, 2015
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    • "Limit of detection (LOD) and limit of quantification (LOQ) were achieved by 1,250 times concentration and 5,000 times concentration for influent and effluent from the WWTPs. They were calculated as the minimum amount of a compound present in the sample that produced a signal to noise ratio of six, based on a 1-μl aliquot injection of the final 0.4 ml extraction solution (Zhou et al. 2009). LOD and LOQ in water were 0.05–0.48 "
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