Antisickling activity of anthocyanins from Bombax pentadrum, Ficus capensis and Ziziphus mucronata: photodegradation effect.
ABSTRACT A survey was conducted in Lubumbashi city (Democratic Republic of Congo) in order to: (a) identify medicinal plants used by traditional healers in the management of sickle cell anaemia, (b) verify their antisickling activity in vitro, (c) determine the most active plants, and (d) verify if anthocyanins are responsible of the bioactivity and study their photodegradation effect.
The Emmel test was used in vitro, for the antisickling activity assays of aqueous and ethanolic extracts of different parts of these plants when a UV lamp and solar irradiations were used to induce the photodegradation effect.
The survey revealed that 13 medicinal plants are used in the treatment of drepanocytosis among which 12 plants exhibited the in vitro antisickling activity for at least one of the used parts or extracts. These plants are Bombax pentadrum, Bougainvillea sp., Byarsocarpus orientalis, Dalberigia bochmintaub, Diplorrhynbchus condolocarpus, Euphorbia heterophylla, Ficus capensis, Harungana madagascariensis, Parinari mobola, Pothmania witfchidii, Syzygium guineense, Temnocalys verdickii and Ziziphus mucronata of which four (Bombax pentadrum, Ficus capensis, Parinari mobola and Ziziphus mucronata) revealed a high antisickling activity. The biological activity of three of these plants is due to anthocyanins. The antisickling activity and photodegradation effect of anthocyanins extracts were studied and minimal concentration of normalization determined. The biological activity of Bombax pentadrum anthocyanins decreased to half of its value after 40 min of irradiation under a lamp emitting at a wavelength of 365 nm and after about 10h of solar irradiation. For Ziziphus mucronata and Ficus capensis, the antisickling activity decreased to half after about 6h under a lamp exposition and after about 50h of solar exposition.
In vitro Antisickling activity justifies the use of these plants by traditional healers and this activity would be due to anthocyanins. But these natural pigments are instable towards UV-Visible irradiations. The conservation of these plants should then be performed in a shield from the sun radiation.
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ABSTRACT: Amino acid analysis showed that solvent extracts of Cajanus cajan L. Millsp. (Fabaceae) seeds (white species) contain, as free amino acid, as much as 26.3% phenylalanine. Antisickling experiments based on the estimated amount of free phenylalanine in the methanol (water-soluble) extract of the seeds showed that the presence of this amino acid alone could account for about 70% of the antisickling potency of Cajanus cajan seed extract.Planta Medica 03/1990; 56(1):41-3. · 2.35 Impact Factor
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ABSTRACT: The leaves of Hymenocardia acida are commonly used in Northern Nigeria alone or in combination with other plant parts to manage sickle cell disease. Phytochemical screening and antisickling studies were carried out. The phytochemical screening revealed the presence of carbohydrates, tannins, flavonoids, saponins, alkaloids, cardiac glycosides, resins, steroids and terpenes. The leaves ethanol extracts at 0.5, 1.0 and 2.0% w/v were observed to reverse sickled human Red Blood Cells (RBC) using microscopic technique. The antisickling activity was found to be dose dependent. The fractions containing flavonoids, saponins and carboxylic acids were found to be responsible for reversal of the sickled RBC. Therefore, the use of the plant by the traditional medical practitioners in the treatment of sickle cell anaemic patients is justified.Pakistan Journal of Biological Sciences. 01/2007;